JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB325649

Anti-ASF1B (phospho S198) antibody [EPR28822-77]

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal ASF1B phospho S198 antibody. Suitable for I-ELISA, Dot, WB and reacts with Synthetic peptide - Human, Human samples.

View Alternative Names

Histone chaperone ASF1B, Anti-silencing function protein 1 homolog B, CCG1-interacting factor A-II, hAsf1, hAsf1b, CIA-II, hCIA-II

5 Images
Western blot - Anti-ASF1B (phospho S198) antibody [EPR28822-77] (AB325649)
  • WB

Lab

Western blot - Anti-ASF1B (phospho S198) antibody [EPR28822-77] (AB325649)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The identity of the bands higher than 37 kDa are unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-ASF1B (phospho S198) antibody [EPR28822-77] (ab325649) at 1/1000 dilution

Lane 1:

Untreated HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate 20 µg (untreated membrane)

Lane 2:

HeLa treated with 100ng/ml Calyculin A for 30 min whole cell lysate 20 µg (untreated membrane)

Lane 3:

Untreated HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate 20 µg (alkaline phosphatase treated membrane)

Lane 4:

HeLa treated with 100ng/ml Calyculin A for 30 min whole cell lysate 20 µg ((alkaline phosphatase treated membrane))

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 18 kDa,20 kDa,36 kDa

false

Exposure time: 180s

Western blot - Anti-ASF1B (phospho S198) antibody [EPR28822-77] (AB325649)
  • WB

Lab

Western blot - Anti-ASF1B (phospho S198) antibody [EPR28822-77] (AB325649)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The identity of the bands higher than 100 kDa are unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-ASF1B (phospho S198) antibody [EPR28822-77] (ab325649) at 1/1000 dilution

Lane 1:

Untreated Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate at 20 µg

Lane 2:

Raji treated with 100ng/ml Calyculin A for 30 min whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 18 kDa,20 kDa,36 kDa

true

Exposure time: 180s

Western blot - Anti-ASF1B (phospho S198) antibody [EPR28822-77] (AB325649)
  • WB

Lab

Western blot - Anti-ASF1B (phospho S198) antibody [EPR28822-77] (AB325649)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 29478807, 11470414). The molecular weight of ASF1A is upshifted when phosphorylated.

To minimize protein degradation, the fresh lysates were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

The identity of the bands higher than 37 kDa are unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-ASF1B (phospho S198) antibody [EPR28822-77] (ab325649) at 1/1000 dilution

Lane 1:

Untreated HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 48 µg

Lane 2:

HeLa treated with 100ng/ml Calyculin A for 30 min whole cell lysate at 48 µg

Lane 3:

HeLa transfected with scrambled siRNA control treated with 100ng/ml Calyculin A for 30 min whole cell lysate at 48 µg

Lane 4:

HeLa transfected with siRNA specifically targeting ASF1B treated with 100ng/ml Calyculin A for 30 min whole cell lysate at 48 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 18 kDa,20 kDa,36 kDa

false

Exposure time: 147s

Indirect ELISA - Anti-ASF1B (phospho S198) antibody [EPR28822-77] (AB325649)
  • I-ELISA

Lab

Indirect ELISA - Anti-ASF1B (phospho S198) antibody [EPR28822-77] (AB325649)

Indirect ELISA analysis of ab325649 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1 : 2500 dilution dilution.

Antigen : Human ASF1B (phospho S198) peptide a;Human ASF1B (phospho S198) peptide b;Human ASF1B (non-phospho) peptide;Human ASF1B (phospho S169) peptide;Human ASF1B (phospho S201) peptide.

Antigen concentration : 1000 ng/ml

Dot Blot - Anti-ASF1B (phospho S198) antibody [EPR28822-77] (AB325649)
  • Dot

Lab

Dot Blot - Anti-ASF1B (phospho S198) antibody [EPR28822-77] (AB325649)

Dot blot analysis of ASF1b using ab325649 at 1 : 1000 (0.492 µg/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution.

Lane 1 : Human ASF1B (phospho S198) peptide a
Lane 2 : Human ASF1B (phospho S198) peptide b
Lane 3 : Human ASF1B (non-phospho) peptide
Lane 4 : Human ASF1B (phospho S169) peptide
Lane 5 : Human ASF1B (phospho S201) peptide

Exposure time : 180 seconds.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Dot Blot - Anti-ASF1B (phospho S198) antibody [EPR28822-77] (ab325649) at 1/1000 dilution

Lane 1:

Human ASF1B (phospho S198) peptide a

Lane 2:

Human ASF1B (phospho S198) peptide b

Lane 3:

Human ASF1B (non-phospho) peptide

Lane 4:

Human ASF1B (phospho S169) peptide

Lane 5:

Human ASF1B (phospho S201) peptide

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 180s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR28822-77

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Dot, I-ELISA, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Synthetic peptide - Human": { "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "250 ng/mL", "IELISA-species-notes": "<p></p>", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly (PubMed : 11897662, PubMed : 14718166, PubMed : 15664198, PubMed : 16151251, PubMed : 21454524, PubMed : 26527279). Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly (PubMed : 11897662, PubMed : 14718166, PubMed : 15664198, PubMed : 16151251). Also involved in the nuclear import of the histone H3-H4 dimer together with importin-4 (IPO4) : specifically recognizes and binds newly synthesized histones with the monomethylation of H3 'Lys-9' (H3K9me1) and diacetylation at 'Lys-5' and 'Lys-12' of H4 (H4K5K12ac) marks in the cytosol (PubMed : 20953179, PubMed : 21454524, PubMed : 26527279). Does not participate in replication-independent nucleosome deposition which is mediated by ASF1A and HIRA (PubMed : 11897662, PubMed : 14718166, PubMed : 15664198, PubMed : 16151251). Required for gonad development (PubMed : 12842904).
See full target information ASF1B phospho S198
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com