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Mouse Monoclonal ASFV p54 antibody. Suitable for IHC-P, ICC/IF, WB and reacts with Transfected cell line samples.

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Images

Western blot - Anti-ASFV p54 antibody [GT853] (AB308411), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASFV p54 antibody [GT853] (AB308411), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-ASFV p54 antibody [GT853] (AB308411), expandable thumbnail

Key facts

Isotype

IgG1

Host species

Mouse

Storage buffer

pH: 7
Constituents: 80% PBS, 20% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Monoclonal

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IFWB
Transfected cell line
Tested
Tested
Tested

Tested
Tested

Species

Transfected cell line

Dilution info

1/1000

Notes

-

Tested
Tested

Species

Transfected cell line

Dilution info

1/1000

Notes

-

Tested
Tested

Species

Transfected cell line

Dilution info

1/5000

Notes

-

Mouse Monoclonal ASFV p54 antibody. Suitable for IHC-P, ICC/IF, WB and reacts with Transfected cell line samples.

Key facts

Isotype

IgG1

Form

Liquid

Clonality

Monoclonal

Clone number

GT853

Purification technique

Affinity purification Protein G

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The ASFV p54 also known as pEP153R is a structural protein of African Swine Fever Virus (ASFV) with a mass of approximately 25 kDa. It is expressed on the surface of the virus functioning as an important component for the virus life cycle. ASFV p54 protein plays a central role in virus attachment and entry into the host cells. It interacts with specific host receptors facilitating the fusion of the virus with cellular membranes and allowing viral particles to enter the host cell cytoplasm.

Biological function summary

ASFV p54 contributes to viral replication and assembly within the infected host cells. It does not function alone but is part of a larger protein complex that includes several other viral proteins. This complex is important for the architecture and stability of the viral envelope ensuring that newly synthesized viral particles are properly formed and can efficiently infect other cells.

Pathways

ASFV p54 plays an important part in pathways related to viral invasion and immune evasion. It is involved in interactions that help suppress host immune responses granting the virus an advantage in persisting within the host. The functions of ASFV p54 are closely tied to those of other structural proteins like p30 which also participate in the processes of viral adhesion and immune system modulation.

Associated diseases and disorders

ASFV p54 is directly associated with African Swine Fever a devastating disease affecting domestic and wild pigs. The function of ASFV p54 makes it a target of interest in understanding the mechanisms of ASFV pathogenesis. This protein interacts with viral components such as the B646L (p72) protein which is implicated in virulence and the disease severity. Targeting ASFV p54 through research or vaccine development may help control the spread of this highly contagious disease in swine populations.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-ASFV p54 antibody [GT853] (ab308411), expandable thumbnail

    Western blot - Anti-ASFV p54 antibody [GT853] (ab308411)

    Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with ASFV p54 antibody [GT853] (ab308411) diluted at 1/5000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

    All lanes: Western blot - Anti-ASFV p54 antibody [GT853] (ab308411) at 1/5000 dilution

    Lane 1: Non-transfected (–) 293T whole cell extracts at 30 µg

    Lane 2: Transfected (+) 293T whole cell extracts at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-mouse IgG antibody

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASFV p54 antibody [GT853] (ab308411), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASFV p54 antibody [GT853] (ab308411)

    Immunohistochemical analysis of African swine fever virus envelope protein p54 transfected HEK293T cell FFPE Cell Pellet Block labeling ASFV p54 (green) using ab308411 at 1/1000 dilution. Antigen retrival was performed using Citrate buffer, pH 6.0, 15 min

  • Immunocytochemistry/ Immunofluorescence - Anti-ASFV p54 antibody [GT853] (ab308411), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-ASFV p54 antibody [GT853] (ab308411)

    For immunofluorescence analysis, mock and transfected HEK293T cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes and permeabilized for detection of ASFV p54 using ab308411 at 1/1000 dilution (green). Blue: Fluoroshield with DAPI.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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