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AB308411

Anti-ASFV p54 antibody [GT853] - BSA and Azide free

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Mouse Monoclonal ASFV p54 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB, sELISA and reacts with Transfected cell line - African swine fever virus, African swine fever virus samples.
3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASFV p54 antibody [GT853] - BSA and Azide free (AB308411)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASFV p54 antibody [GT853] - BSA and Azide free (AB308411)

Immunohistochemical analysis of African swine fever virus envelope protein p54 transfected HEK293T cell FFPE Cell Pellet Block labeling ASFV p54 (green) using ab308411 at 1/1000 dilution. Antigen retrival was performed using Citrate buffer, pH 6.0, 15 min

Immunocytochemistry/ Immunofluorescence - Anti-ASFV p54 antibody [GT853] - BSA and Azide free (AB308411)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ASFV p54 antibody [GT853] - BSA and Azide free (AB308411)

For immunofluorescence analysis, mock and transfected HEK293T cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes and permeabilized for detection of ASFV p54 using ab308411 at 1/1000 dilution (green). Blue : Fluoroshield with DAPI.

Sandwich ELISA - Anti-ASFV p54 antibody [GT853] - BSA and Azide free (AB308411)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-ASFV p54 antibody [GT853] - BSA and Azide free (AB308411)

Sandwich ELISA detection of recombinant ASFV p54 using antibodies as below.
Capture : ab308411 (5 µg/mL)
Detection : HRP conjugated ASFV p54 antibody (1 µg/mL).

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

GT853

Isotype

IgG1

Carrier free

Yes

Reacts with

African swine fever virus

Applications

IHC-P, ICC/IF, sELISA, WB

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "African swine fever virus": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" }, "Transfected cell line - African swine fever virus": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "sELISA-species-checked": "notRecommended", "sELISA-species-dilution-info": "", "sELISA-species-notes": "" } } }
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Product details

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ASFV p54 also known as pEP153R is a structural protein of African Swine Fever Virus (ASFV) with a mass of approximately 25 kDa. It is expressed on the surface of the virus functioning as an important component for the virus life cycle. ASFV p54 protein plays a central role in virus attachment and entry into the host cells. It interacts with specific host receptors facilitating the fusion of the virus with cellular membranes and allowing viral particles to enter the host cell cytoplasm.
Biological function summary

ASFV p54 contributes to viral replication and assembly within the infected host cells. It does not function alone but is part of a larger protein complex that includes several other viral proteins. This complex is important for the architecture and stability of the viral envelope ensuring that newly synthesized viral particles are properly formed and can efficiently infect other cells.

Pathways

ASFV p54 plays an important part in pathways related to viral invasion and immune evasion. It is involved in interactions that help suppress host immune responses granting the virus an advantage in persisting within the host. The functions of ASFV p54 are closely tied to those of other structural proteins like p30 which also participate in the processes of viral adhesion and immune system modulation.

ASFV p54 is directly associated with African Swine Fever a devastating disease affecting domestic and wild pigs. The function of ASFV p54 makes it a target of interest in understanding the mechanisms of ASFV pathogenesis. This protein interacts with viral components such as the B646L (p72) protein which is implicated in virulence and the disease severity. Targeting ASFV p54 through research or vaccine development may help control the spread of this highly contagious disease in swine populations.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information ASFV p54
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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