Anti-ASFV p54 antibody [GT853] - BSA and Azide free

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASFV p54 antibody [GT853] - BSA and Azide free (AB308411)

Immunohistochemical analysis of African swine fever virus envelope protein p54 transfected HEK293T cell FFPE Cell Pellet Block labeling ASFV p54 (green) using ab308411 at 1/1000 dilution. Antigen retrival was performed using Citrate buffer, pH 6.0, 15 min

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-ASFV p54 antibody [GT853] - BSA and Azide free (AB308411)

For immunofluorescence analysis, mock and transfected HEK293T cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes and permeabilized for detection of ASFV p54 using ab308411 at 1/1000 dilution (green). Blue : Fluoroshield with DAPI.

• sELISA

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Sandwich ELISA - Anti-ASFV p54 antibody [GT853] - BSA and Azide free (AB308411)

Sandwich ELISA detection of recombinant ASFV p54 using antibodies as below.
Capture : ab308411 (5 µg/mL)
Detection : HRP conjugated ASFV p54 antibody (1 µg/mL).

• WB

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Western blot - Anti-ASFV p54 antibody [GT853] - BSA and Azide free (AB308411)

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with ASFV p54 antibody [GT853] (ab308411) diluted at 1/5000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-ASFV p54 antibody [GT853] - BSA and Azide free (ab308411) at 1/5000 dilution

Lane 1:

Non-transfected (–) 293T whole cell extracts at 30 µg

Lane 2:

Transfected (+) 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-mouse IgG antibody

Predicted band size: 30 kDa

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