Rabbit Recombinant Monoclonal ASFV p54 antibody. Carrier free. Suitable for ICC/IF, WB, sELISA and reacts with Transfected cell line - African swine fever virus samples.
Images
![Sandwich ELISA - Anti-ASFV p54 antibody [HL1287] - BSA and Azide free (AB308356), expandable thumbnail](https://content.abcam.com/products/images/asfv-p54-antibody-hl1287-bsa-and-azide-free-ab308356--sandwich-elisa-img440722.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-ASFV p54 antibody [HL1287] - BSA and Azide free (AB308356)")
![Western blot - Anti-ASFV p54 antibody [HL1287] - BSA and Azide free (AB308356), expandable thumbnail](https://content.abcam.com/products/images/asfv-p54-antibody-hl1287-bsa-and-azide-free-ab308356--western-blot-img408080.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ASFV p54 antibody [HL1287] - BSA and Azide free (AB308356)")
![Immunocytochemistry/ Immunofluorescence - Anti-ASFV p54 antibody [HL1287] - BSA and Azide free (AB308356), expandable thumbnail](https://content.abcam.com/products/images/asfv-p54-antibody-hl1287-bsa-and-azide-free-ab308356--immunocytochemistry-immunofluorescence-img408079.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-ASFV p54 antibody [HL1287] - BSA and Azide free (AB308356)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Reactivity data
Select an application| ICC/IF | WB | sELISA | |
|---|---|---|---|
| African swine fever virus | Predicted | Predicted | Predicted |
| Transfected cell line - African swine fever virus | Tested | Tested | Tested |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - African swine fever virus | Dilution info 1/500 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species African swine fever virus | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - African swine fever virus | Dilution info 1/5000 - 1/10000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species African swine fever virus | Dilution info - | Notes - |
sELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - African swine fever virus | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species African swine fever virus | Dilution info - | Notes - |
Target data
Rabbit Recombinant Monoclonal ASFV p54 antibody. Carrier free. Suitable for ICC/IF, WB, sELISA and reacts with Transfected cell line - African swine fever virus samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Carrier free
- Yes
- Clone number
- HL1287
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The ASFV p54 also known as pEP153R is a structural protein of African Swine Fever Virus (ASFV) with a mass of approximately 25 kDa. It is expressed on the surface of the virus functioning as an important component for the virus life cycle. ASFV p54 protein plays a central role in virus attachment and entry into the host cells. It interacts with specific host receptors facilitating the fusion of the virus with cellular membranes and allowing viral particles to enter the host cell cytoplasm.
- Biological function summary
ASFV p54 contributes to viral replication and assembly within the infected host cells. It does not function alone but is part of a larger protein complex that includes several other viral proteins. This complex is important for the architecture and stability of the viral envelope ensuring that newly synthesized viral particles are properly formed and can efficiently infect other cells.
- Pathways
ASFV p54 plays an important part in pathways related to viral invasion and immune evasion. It is involved in interactions that help suppress host immune responses granting the virus an advantage in persisting within the host. The functions of ASFV p54 are closely tied to those of other structural proteins like p30 which also participate in the processes of viral adhesion and immune system modulation.
- Associated diseases and disorders
ASFV p54 is directly associated with African Swine Fever a devastating disease affecting domestic and wild pigs. The function of ASFV p54 makes it a target of interest in understanding the mechanisms of ASFV pathogenesis. This protein interacts with viral components such as the B646L (p72) protein which is implicated in virulence and the disease severity. Targeting ASFV p54 through research or vaccine development may help control the spread of this highly contagious disease in swine populations.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Sandwich ELISA - Anti-ASFV p54 antibody [HL1287] - BSA and Azide free (ab308356), expandable thumbnail](https://content.abcam.com/products/images/asfv-p54-antibody-hl1287-bsa-and-azide-free-ab308356--sandwich-elisa-img440722.jpg "Sandwich ELISA - Anti-ASFV p54 antibody [HL1287] - BSA and Azide free (ab308356)")
Sandwich ELISA - Anti-ASFV p54 antibody [HL1287] - BSA and Azide free (ab308356)
Sandwich ELISA detection of recombinant HEK293 expressed, full-length ASFV p54 using ab308356. Capture: ASFV p54 antibody [HL1289] (ab317085) (5 µg/mL) Detection: HRP-conjugated ASFV p54 antibody [HL1287] (ab308356) (1 µg/mL) Please notice that ab308356 needs to be conjugated to HRP to function as the detection antibody when paired with ab317085.
![Western blot - Anti-ASFV p54 antibody [HL1287] - BSA and Azide free (ab308356), expandable thumbnail](https://content.abcam.com/products/images/asfv-p54-antibody-hl1287-bsa-and-azide-free-ab308356--western-blot-img408080.jpg "Western blot - Anti-ASFV p54 antibody [HL1287] - BSA and Azide free (ab308356)")
Western blot - Anti-ASFV p54 antibody [HL1287] - BSA and Azide free (ab308356)
ASFV p54 Western blot staining using rabbit Anti-ASFV p54 antibody
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with ASFV p54 antibody [HL1287] (ab308356) diluted at 1/100000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
All lanes: Western blot - Anti-ASFV p54 antibody [HL1287] - BSA and Azide free (ab308356) at 1/100000 dilution
Lane 1: Non-transfected (–) 293T whole cell extracts at 30 µg
Lane 2: Transfected (+) 293T whole cell extracts at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
![Immunocytochemistry/ Immunofluorescence - Anti-ASFV p54 antibody [HL1287] - BSA and Azide free (ab308356), expandable thumbnail](https://content.abcam.com/products/images/asfv-p54-antibody-hl1287-bsa-and-azide-free-ab308356--immunocytochemistry-immunofluorescence-img408079.jpg "Immunocytochemistry/ Immunofluorescence - Anti-ASFV p54 antibody [HL1287] - BSA and Azide free (ab308356)")
Immunocytochemistry/ Immunofluorescence - Anti-ASFV p54 antibody [HL1287] - BSA and Azide free (ab308356)
ASFV p54 Immunocytochemistry/ Immunofluorescence staining of HEK293T cells using rabbit Anti-ASFV p54 antibody
For immunofluorescence analysis, mock and transfected HEK293T cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes and permeabilized for detection of ASFV p54 using ab308356 at 1/500 dilution (green). Blue: Fluoroshield with DAPI.
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