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AB308356

Anti-ASFV p54 antibody [HL1287] - BSA and Azide free

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Rabbit Recombinant Monoclonal ASFV p54 antibody. Carrier free. Suitable for ICC/IF, WB, sELISA and reacts with Transfected cell line - African swine fever virus samples.
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Immunocytochemistry/ Immunofluorescence - Anti-ASFV p54 antibody [HL1287] - BSA and Azide free (AB308356)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ASFV p54 antibody [HL1287] - BSA and Azide free (AB308356)

For immunofluorescence analysis, mock and transfected HEK293T cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes and permeabilized for detection of ASFV p54 using ab308356 at 1/500 dilution (green). Blue : Fluoroshield with DAPI.

Sandwich ELISA - Anti-ASFV p54 antibody [HL1287] - BSA and Azide free (AB308356)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-ASFV p54 antibody [HL1287] - BSA and Azide free (AB308356)

Sandwich ELISA detection of recombinant HEK293 expressed, full-length ASFV p54 using ab308356. Capture : ASFV p54 antibody [HL1289] (ab317085) (5 µg/mL) Detection : HRP-conjugated ASFV p54 antibody [HL1287] (ab308356) (1 µg/mL) Please notice that ab308356 needs to be conjugated to HRP to function as the detection antibody when paired with ab317085.

Western blot - Anti-ASFV p54 antibody [HL1287] - BSA and Azide free (AB308356)
  • WB

Supplier Data

Western blot - Anti-ASFV p54 antibody [HL1287] - BSA and Azide free (AB308356)

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with ASFV p54 antibody [HL1287] (ab308356) diluted at 1/100000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-ASFV p54 antibody [HL1287] - BSA and Azide free (ab308356) at 1/100000 dilution

Lane 1:

Non-transfected (–) 293T whole cell extracts at 30 µg

Lane 2:

Transfected (+) 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1287

Isotype

IgG

Carrier free

Yes

Reacts with

African swine fever virus

Applications

ICC/IF, WB, sELISA

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "African swine fever virus": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "sELISA-species-checked": "predicted", "sELISA-species-dilution-info": "", "sELISA-species-notes": "" }, "Transfected cell line - African swine fever virus": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/10000", "WB-species-notes": "<p></p>", "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ASFV p54 also known as pEP153R is a structural protein of African Swine Fever Virus (ASFV) with a mass of approximately 25 kDa. It is expressed on the surface of the virus functioning as an important component for the virus life cycle. ASFV p54 protein plays a central role in virus attachment and entry into the host cells. It interacts with specific host receptors facilitating the fusion of the virus with cellular membranes and allowing viral particles to enter the host cell cytoplasm.
Biological function summary

ASFV p54 contributes to viral replication and assembly within the infected host cells. It does not function alone but is part of a larger protein complex that includes several other viral proteins. This complex is important for the architecture and stability of the viral envelope ensuring that newly synthesized viral particles are properly formed and can efficiently infect other cells.

Pathways

ASFV p54 plays an important part in pathways related to viral invasion and immune evasion. It is involved in interactions that help suppress host immune responses granting the virus an advantage in persisting within the host. The functions of ASFV p54 are closely tied to those of other structural proteins like p30 which also participate in the processes of viral adhesion and immune system modulation.

ASFV p54 is directly associated with African Swine Fever a devastating disease affecting domestic and wild pigs. The function of ASFV p54 makes it a target of interest in understanding the mechanisms of ASFV pathogenesis. This protein interacts with viral components such as the B646L (p72) protein which is implicated in virulence and the disease severity. Targeting ASFV p54 through research or vaccine development may help control the spread of this highly contagious disease in swine populations.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information ASFV p54
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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