AB317085

Anti-ASFV p54 antibody [HL1289]

Recombinant
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Rabbit Recombinant Monoclonal P54 antibody. Suitable for WB, ICC/IF, sELISA, LFA and reacts with Transfected cell lysate - African swine fever virus, Recombinant full length protein - African swine fever virus, Recombinant fragment - African swine fever virus samples. Immunogen corresponding to Recombinant Fragment Protein within African swine fever virus BA71V Ba71V-126.

View Alternative Names

E183L, Inner membrane protein p54, pE183L

11 Images

Immunocytochemistry/ Immunofluorescence - Anti-ASFV p54 antibody [HL1289] (AB317085)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ASFV p54 antibody [HL1289] (AB317085)

ab317085 detects ASFV p54 protein by immunofluorescent analysis.
Sample : Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green : ASFV p54 stained by ab317085 diluted at 1 : 500.
Blue : Fluoroshield with DAPI.

ELISA - Anti-ASFV p54 antibody [HL1289] (AB317085)

ELISA

Supplier Data

ELISA - Anti-ASFV p54 antibody [HL1289] (AB317085)

Sandwich ELISA detection of recombinant HEK293 expressed, full-length ASFV p54 using capture antibody ab317085 at 5 ug/mL and a detection antibody HRP-conjugated ASFV p54 antibody at 1 ug/mL.

ELISA

Supplier Data

ELISA - Anti-ASFV p54 antibody [HL1289] (AB317085)

Sandwich ELISA detection of recombinant HEK293 expressed, full-length ASFV p54 using capture antibody ab317085 at 5 ug/mL and a detection antibody HRP-conjugated ASFV p54 antibody at 1 ug/mL.

ELISA

Supplier Data

ELISA - Anti-ASFV p54 antibody [HL1289] (AB317085)

Sandwich ELISA detection of recombinant HEK293 expressed, full-length ASFV p54 using capture antibody ab317085 at 5 ug/mL and a detection antibody HRP-conjugated ASFV p54 antibody at 1 ug/mL.

ELISA

Supplier Data

ELISA - Anti-ASFV p54 antibody [HL1289] (AB317085)

Sandwich ELISA detection of recombinant HEK293 expressed, full-length ASFV p54, His Tag using capture antibody ASFV p54 antibody at 5 ug/mL and detection antibody HRP-conjugated ab317085 at 1 ug/mL.

Please notice that ab317085 needs to be conjugated to HRP to function as the detection antibody when paired with this capture antibody.

Supplier Data

Lateral flow assay - Anti-ASFV p54 antibody [HL1289] (AB317085)

Detection of HEK293 expressed, ASFV p54 (ECD) protein by lateral flow assay using the monoclonal antibody pair

Capture : ASFV p54 antibody
Detection : ASFV p54 antibody (ab317085).

Samples (40 ng) :
1. ASFV p54 (ECD) protein
2. Lysis buffer

Supplier Data

Lateral flow assay - Anti-ASFV p54 antibody [HL1289] (AB317085)

Detection of HEK293 expressed, ASFV p54 (ECD) protein by lateral flow assay using the monoclonal antibody pair

Capture : ASFV p54 antibody
Detection : ASFV p54 antibody (ab317085).

Samples (40 ng) :
1. ASFV p54 (ECD) protein
2. Lysis buffer

Supplier Data

Lateral flow assay - Anti-ASFV p54 antibody [HL1289] (AB317085)

Detection of HEK293 expressed, ASFV p54 (ECD) protein by lateral flow assay using the monoclonal antibody pair

Capture : ASFV p54 antibody
Detection : ASFV p54 antibody (ab317085).

Samples (80 ng) :
1. ASFV p54 (ECD) protein
2. Lysis buffer

Supplier Data

Lateral flow assay - Anti-ASFV p54 antibody [HL1289] (AB317085)

Detection of HEK293 expressed, ASFV p54 (ECD) protein by lateral flow assay using the monoclonal antibody pair

Capture : ASFV p54 antibody
Detection : ASFV p54 antibody (ab317085).

Samples (20 ng) :
1. ASFV p54 (ECD) protein
2. Lysis buffer

Supplier Data

Lateral flow assay - Anti-ASFV p54 antibody [HL1289] (AB317085)

Detection of E.coli expressed, ASFV p54 (ECD) protein by lateral flow assay using the monoclonal antibody pair.

Capture : ASFV p54 antibody (ab317085)
Detection : ASFV p54 antibody.

Supplier Data

Lateral flow assay - Anti-ASFV p54 antibody [HL1289] (AB317085)

Detection of HEK293 expressed, ASFV p54 (ECD) protein by lateral flow assay using the monoclonal antibody pair

Capture : ASFV p54 antibody
Detection : ASFV p54 antibody (ab317085).

Samples (20 ng) :
1. ASFV p54 (ECD) protein
2. Lysis buffer

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1289

Isotype

IgG

Carrier free

Reacts with

African swine fever virus

Applications

sELISA, LFA, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within African swine fever virus BA71V Ba71V-126.

Q65194

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"}, "LFA" : {"fullname" : "Lateral flow assay", "shortname":"LFA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "African swine fever virus": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "sELISA-species-checked": "predicted", "sELISA-species-dilution-info": "", "sELISA-species-notes": "", "LFA-species-checked": "predicted", "LFA-species-dilution-info": "", "LFA-species-notes": "" }, "Recombinant fragment - African swine fever virus": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "sELISA-species-checked": "notRecommended", "sELISA-species-dilution-info": "", "sELISA-species-notes": "", "LFA-species-checked": "testedAndGuaranteed", "LFA-species-dilution-info": "", "LFA-species-notes": "" }, "Recombinant full length protein - African swine fever virus": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "5 µg/mL", "sELISA-species-notes": "", "LFA-species-checked": "testedAndGuaranteed", "LFA-species-dilution-info": "", "LFA-species-notes": "" }, "Transfected cell lysate - African swine fever virus": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/100000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "", "sELISA-species-checked": "notRecommended", "sELISA-species-dilution-info": "", "sELISA-species-notes": "", "LFA-species-checked": "notRecommended", "LFA-species-dilution-info": "", "LFA-species-notes": "" } } }

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ASFV p54 also known as pEP153R is a structural protein of African Swine Fever Virus (ASFV) with a mass of approximately 25 kDa. It is expressed on the surface of the virus functioning as an important component for the virus life cycle. ASFV p54 protein plays a central role in virus attachment and entry into the host cells. It interacts with specific host receptors facilitating the fusion of the virus with cellular membranes and allowing viral particles to enter the host cell cytoplasm.

Biological function summary

ASFV p54 contributes to viral replication and assembly within the infected host cells. It does not function alone but is part of a larger protein complex that includes several other viral proteins. This complex is important for the architecture and stability of the viral envelope ensuring that newly synthesized viral particles are properly formed and can efficiently infect other cells.

Pathways

ASFV p54 plays an important part in pathways related to viral invasion and immune evasion. It is involved in interactions that help suppress host immune responses granting the virus an advantage in persisting within the host. The functions of ASFV p54 are closely tied to those of other structural proteins like p30 which also participate in the processes of viral adhesion and immune system modulation.

ASFV p54 is directly associated with African Swine Fever a devastating disease affecting domestic and wild pigs. The function of ASFV p54 makes it a target of interest in understanding the mechanisms of ASFV pathogenesis. This protein interacts with viral components such as the B646L (p72) protein which is implicated in virulence and the disease severity. Targeting ASFV p54 through research or vaccine development may help control the spread of this highly contagious disease in swine populations.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Inner envelope protein involved, through its interaction with host dynein, in the intracellular microtubule-dependent transport of viral capsid toward viral factories (PubMed : 11559815). Seems to induce caspase-3 activation and apoptosis (PubMed : 15225638). Plays a role in virion morphogenesis by recruiting and transforming the host ER membranes into the precursors of the viral envelope (PubMed : 15047843). Involved in virus attachment to the host cell (PubMed : 8764090, PubMed : 9568043).

See full target information Ba71V-126

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ASFV p54 antibody [HL1289]

Immunocytochemistry/ Immunofluorescence - Anti-ASFV p54 antibody [HL1289] (AB317085)

ELISA - Anti-ASFV p54 antibody [HL1289] (AB317085)

ELISA - Anti-ASFV p54 antibody [HL1289] (AB317085)

ELISA - Anti-ASFV p54 antibody [HL1289] (AB317085)

ELISA - Anti-ASFV p54 antibody [HL1289] (AB317085)

Lateral flow assay - Anti-ASFV p54 antibody [HL1289] (AB317085)

Lateral flow assay - Anti-ASFV p54 antibody [HL1289] (AB317085)

Lateral flow assay - Anti-ASFV p54 antibody [HL1289] (AB317085)

Lateral flow assay - Anti-ASFV p54 antibody [HL1289] (AB317085)

Lateral flow assay - Anti-ASFV p54 antibody [HL1289] (AB317085)

Lateral flow assay - Anti-ASFV p54 antibody [HL1289] (AB317085)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Proteins & Peptides

Primary Antibodies

Proteins & Peptides

Assay Kits

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise