Anti-ASGR2 antibody [EPR16975]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASGR2 antibody [EPR16975] (AB200196)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling ASGR2 with ab200196 at 1/600 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.

Cytoplasm and cell membrane staining on Human liver tissue is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASGR2 antibody [EPR16975] (AB200196)

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling ASGR2 with ab200196 at 1/600 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.

No staining on Human skeletal muscle tissue is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IP

Supplier Data

Immunoprecipitation - Anti-ASGR2 antibody [EPR16975] (AB200196)

ASGR2 was immunoprecipitated from 1mg of Human fetal liver whole cell lysate with ab200196 at 1/30 dilution.

Western blot was performed from the immunoprecipitate using ab200196 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

Lane 1 : Human fetal liver whole cell lysate 10 μg (Input).

Lane 2 : ab200196 IP in Human fetal liver whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab200196 in Human fetal liver whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-ASGR2 antibody [EPR16975] (ab200196)

Predicted band size: 35 kDa

false

• WB

Supplier Data

Western blot - Anti-ASGR2 antibody [EPR16975] (AB200196)

The observed MW is consistent with what has been described in the literature (PMID : 3040719).

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-ASGR2 antibody [EPR16975] (ab200196) at 1/5000 dilution

Lane 1:

Human fetal liver lysate at 10 µg

Lane 2:

K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysate at 10 µg

Lane 3:

HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 10 µg

Lane 4:

MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 24 kDa,27 kDa,35 kDa

Observed band size: 46 kDa

false

Exposure time: 30s