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AB234745

Anti-ASH1L antibody

3

(1 Review)

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(1 Publication)

Rabbit Polyclonal ASH1L antibody. Suitable for ICC/IF, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ASH1L aa 1900 to C-terminus.

View Alternative Names

KIAA1420, KMT2H, ASH1L, Histone-lysine N-methyltransferase ASH1L, ASH1-like protein, Absent small and homeotic disks protein 1 homolog, Lysine N-methyltransferase 2H, huASH1

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-ASH1L antibody (AB234745)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ASH1L antibody (AB234745)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells labelling KMT2H / ASH1 (green) using ab234745 at 1/100 dilution in ICC/IF analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASH1L antibody (AB234745)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASH1L antibody (AB234745)

Paraffin-embedded human glioma tissue stained for KMT2H / ASH1 using ab234745 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASH1L antibody (AB234745)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASH1L antibody (AB234745)

Paraffin-embedded human melanoma tissue stained for KMT2H / ASH1 using ab234745 at 1/100 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human ASH1L aa 1900 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9NR48

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ASH1L also known as Absent Small or Homeotic-Like 1 is a protein-coding gene that functions as a histone lysine methyltransferase. This enzyme has a large mass of around 296 kDa and is mainly expressed in the human brain and the testes. It is located in the euchromatic region and participates in depositing methyl groups on lysine residues of histones which influences chromatin structure and gene expression. Due to its ability to modify chromatin ASH1L plays a significant role in regulating transcription.
Biological function summary

ASH1L contributes to various processes involving transcriptional regulation and chromatin modification. This protein functions as part of the Set1/Ash1 complex where it influences gene expression by specifically methylating histone H3 at lysine 36 (H3K36). The methylation of this residue is associated with active transcription and is important for the proper regulation of genes involved in development and differentiation. ASH1L-mediated modifications thereby facilitate the global maintenance of the active chromatin state essential for cellular processes.

Pathways

ASH1L is engaged in pathways related to histone modification and transcriptional regulation. It functions alongside proteins in the Wnt signaling pathway which plays a vital role in regulating tissue-specific transcription programs. ASH1L can alternatively interact with other histone methyltransferases like the NSD family which share similar functions in chromatin dynamics and are involved in transcription elongation and chromatin remodeling.

ASH1L has been associated with developmental disorders and certain cancers. Its function in epigenetic regulation makes it significant in neurodevelopmental disorders where disruptions in its expression or function can lead to conditions such as autism spectrum disorders. Additionally ASH1L's misregulation has been linked to leukemia where it interacts with mixed-lineage leukemia (MLL) proteins due to its role in regulating gene expression involved in cell proliferation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Histone methyltransferase specifically trimethylating 'Lys-36' of histone H3 forming H3K36me3 (PubMed : 21239497). Also monomethylates 'Lys-9' of histone H3 (H3K9me1) in vitro (By similarity). The physiological significance of the H3K9me1 activity is unclear (By similarity).
See full target information ASH1L
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2412233 PubMed39680752

2024

SREBP1c-Mediated Transcriptional Repression of YME1L1 Contributes to Acute Kidney Injury by Inducing Mitochondrial Dysfunction in Tubular Epithelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Wang Xin,Jie Zhou,Yuzhu Peng,Shuiqin Gong,Wenhao Liao,Yaqin Wang,Xixin Huang,Yang Mao,Mengying Yao,Shaozong Qin,Jiachuan Xiong,Yan Li,Qigang Lan,Yinghui Huang,Jinghong Zhao
View all publications
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Product promise

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