JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB50981

Anti-ASH1L antibody [ASH5H03]

Be the first to review this product! Submit a review

|

(4 Publications)

Mouse Monoclonal ASH1L antibody. Suitable for WB and reacts with Recombinant fragment - Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ASH1L aa 1600 to C-terminus.

View Alternative Names

KIAA1420, KMT2H, ASH1L, Histone-lysine N-methyltransferase ASH1L, ASH1-like protein, Absent small and homeotic disks protein 1 homolog, Lysine N-methyltransferase 2H, huASH1

1 Images
Western blot - Anti-ASH1L antibody [ASH5H03] (AB50981)
  • WB

Unknown

Western blot - Anti-ASH1L antibody [ASH5H03] (AB50981)

This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

All lanes:

Western blot - Anti-ASH1L antibody [ASH5H03] (ab50981)

All lanes:

Recombinant ASH1L (immunogen)

Predicted band size: 333 kDa

Observed band size: 35 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

ASH5H03

Isotype

IgG1

Carrier free

No

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human ASH1L aa 1600 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9NR48

style
left-column

Complementary Products

Primary Antibodies

AB32400

Anti-KMT2A / MLL antibody [mmN4.4]

BOND RX™ Validated|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT2A / MLL antibody [mmN4.4] (AB32400)

  • 1
  • 1
Primary Antibodies

AB38113

Anti-KDM6B / JMJD3 antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM6B / JMJD3 antibody (AB38113)

  • 3
  • 10
Primary Antibodies

AB36938

Anti-KDM6A / UTX antibody

Immunocytochemistry/ Immunofluorescence - Anti-KDM6A / UTX antibody (AB36938)

  • 3
  • 4
Primary Antibodies

AB8580

Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade

BOND RX™ Validated

ChIP - Anti-Histone H3 (tri methyl K4) antibody - ChIP Grade (AB8580)

  • 5
  • 90
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant fragment - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
ab50981 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum
Storage buffer
pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 1% BSA, 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.03% Tripotassium orthophosphate, 0.0225% Potassium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ASH1L also known as Absent Small or Homeotic-Like 1 is a protein-coding gene that functions as a histone lysine methyltransferase. This enzyme has a large mass of around 296 kDa and is mainly expressed in the human brain and the testes. It is located in the euchromatic region and participates in depositing methyl groups on lysine residues of histones which influences chromatin structure and gene expression. Due to its ability to modify chromatin ASH1L plays a significant role in regulating transcription.
Biological function summary

ASH1L contributes to various processes involving transcriptional regulation and chromatin modification. This protein functions as part of the Set1/Ash1 complex where it influences gene expression by specifically methylating histone H3 at lysine 36 (H3K36). The methylation of this residue is associated with active transcription and is important for the proper regulation of genes involved in development and differentiation. ASH1L-mediated modifications thereby facilitate the global maintenance of the active chromatin state essential for cellular processes.

Pathways

ASH1L is engaged in pathways related to histone modification and transcriptional regulation. It functions alongside proteins in the Wnt signaling pathway which plays a vital role in regulating tissue-specific transcription programs. ASH1L can alternatively interact with other histone methyltransferases like the NSD family which share similar functions in chromatin dynamics and are involved in transcription elongation and chromatin remodeling.

ASH1L has been associated with developmental disorders and certain cancers. Its function in epigenetic regulation makes it significant in neurodevelopmental disorders where disruptions in its expression or function can lead to conditions such as autism spectrum disorders. Additionally ASH1L's misregulation has been linked to leukemia where it interacts with mixed-lineage leukemia (MLL) proteins due to its role in regulating gene expression involved in cell proliferation.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Histone methyltransferase specifically trimethylating 'Lys-36' of histone H3 forming H3K36me3 (PubMed : 21239497). Also monomethylates 'Lys-9' of histone H3 (H3K9me1) in vitro (By similarity). The physiological significance of the H3K9me1 activity is unclear (By similarity).
See full target information ASH1L
style
left-column

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Discover oncology 16:1070 PubMed40504347

2025

Comprehensive multi-omics analysis of histone acetylation modulators identifies ASH1L as a novel aggressive marker for osteosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Chenlie Ni,Qiwen Sun,Haibo Yin

Cell 175:1887-1901.e18 PubMed30550787

2018

Asymmetric Expression of LincGET Biases Cell Fate in Two-Cell Mouse Embryos.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaqiang Wang,Leyun Wang,Guihai Feng,Yukai Wang,Yufei Li,Xin Li,Chao Liu,Guanyi Jiao,Cheng Huang,Junchao Shi,Tong Zhou,Qi Chen,Zhonghua Liu,Wei Li,Qi Zhou

PloS one 9:e112384 PubMed25380300

2014

Mutant MMP-9 and HGF gene transfer enhance resolution of CCl4-induced liver fibrosis in rats: role of ASH1 and EZH2 methyltransferases repression.

Applications

Dot

Species

Unspecified reactive species

Hussein Atta,Mahmoud El-Rehany,Olfat Hammam,Hend Abdel-Ghany,Maggie Ramzy,Martin Roderfeld,Elke Roeb,Ayman Al-Hendy,Salama Abdel Raheim,Hatem Allam,Heba Marey

Hepatology (Baltimore, Md.) 56:1129-39 PubMed22488473

2012

Histone methyltransferase ASH1 orchestrates fibrogenic gene transcription during myofibroblast transdifferentiation.

Applications

WB

Species

Unspecified reactive species

Maria Jesus Perugorria,Caroline L Wilson,Mujdat Zeybel,Meagan Walsh,Shilu Amin,Stuart Robinson,Steven A White,Alastair D Burt,Fiona Oakley,Hidekazu Tsukamoto,Derek A Mann,Jelena Mann
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com