AB181117

Anti-ASH2L antibody [EPR13106]

RabMAb
Recombinant
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(3 Publications )

Rabbit Recombinant Monoclonal ASH2L antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Rat samples. Cited in 3 publications.

View Alternative Names

ASH2L1, ASH2L, Set1/Ash2 histone methyltransferase complex subunit ASH2, ASH2-like protein

4 Images

Flow Cytometry (Intracellular) - Anti-ASH2L antibody [EPR13106] (AB181117)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-ASH2L antibody [EPR13106] (AB181117)

Intracellular flow cytometric analysis of2% paraformaldehyde fixed HeLa cells labeling ASH2L with ab181117 at 1/20 followed byGoat anti rabbit IgG (FITC) at 1/150 (pink).Rabbit monoclonal IgG was used as Isotype control (green).

Immunocytochemistry/ Immunofluorescence - Anti-ASH2L antibody [EPR13106] (AB181117)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ASH2L antibody [EPR13106] (AB181117)

Immunofluorescent analysis of acetone fixed HeLa cells labeling ASH2L with ab181117 at 1/100 (green). Staining with Dapi is shown in blue.

Supplier Data

Immunoprecipitation - Anti-ASH2L antibody [EPR13106] (AB181117)

Immunoprecipitation analysis of 293T cells labeling ASH2L using ab181117 at 1/60 dilution. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/2000 was used as secondary antibody.

All lanes:

Immunoprecipitation - Anti-ASH2L antibody [EPR13106] (ab181117)

Predicted band size: 69 kDa

Observed band size: 50 kDa,80 kDa

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Supplier Data

Western blot - Anti-ASH2L antibody [EPR13106] (AB181117)

All lanes:

Western blot - Anti-ASH2L antibody [EPR13106] (ab181117) at 1/10000 dilution

Lane 1:

293T cell lysate at 20 µg

Lane 2:

K562 cell lysate at 20 µg

Lane 3:

C6 cell lysate at 20 µg

Lane 4:

PC-12 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 69 kDa

Observed band size: 80 kDa

false

Carrier free

Anti-ASH2L antibody [EPR13106] - BSA and Azide free
HRP

HRP Anti-ASH2L antibody [EPR13106]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13106

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

IP, WB, Flow Cyt (Intra), ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/60", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/20", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "1/60", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "1/20", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ASH2L also known as ASH2 is a protein with a mass of approximately 75 kDa. It functions as part of the trithorax group (TrxG) complex specifically the COMPASS-like complex where it plays an important role in histone methylation. This methylation occurs at histone H3 on lysine 4 (H3K4) an important modification for transcriptional activation. ASH2L expresses in various tissues with significant levels in the nucleus due to its involvement in chromatin modification.

Biological function summary

ASH2L participates in the regulation of gene expression. It acts as a core subunit of the SET/MLL family of H3K4 methyltransferases which form part of larger COMPASS-like complexes. These complexes modulate chromatin structure influencing transcriptional activation by methylating histone H3 at lysine 4. This modification is essential for the transition from transcriptional repression to activation during development and cell differentiation. ASH2L's role in these processes highlights its importance in maintaining proper gene expression patterns.

Pathways

ASH2L participates in the Wnt signaling pathway and influences the cell cycle. In the Wnt pathway ASH2L interacts with proteins such as β-catenin to modulate the transcription of target genes. It also affects the methylation status of promoters in various signaling pathways contributing to the regulation of gene expression involved in cell proliferation and differentiation. Additionally ASH2L is functionally connected to other proteins like MLL1 which shares involvement in chromatin remodeling and is important for epigenetic regulation.

ASH2L association with cancer and neurodevelopmental disorders is significant. Changes in ASH2L expression or function can lead to dysregulation of gene expression promoting oncogenesis or developmental abnormalities. In some cancers ASH2L's interaction with MLL can result in altered histone marks affecting tumor suppressor or oncogene expression. Besides cancer neurodevelopmental disorders like intellectual disability may have links to ASH2L due to its role in regulating genes involved in brain development. Understanding ASH2L's interactions and dysfunctions in these contexts provides insight into potential therapeutic approaches.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcriptional regulator (PubMed : 12670868). Component or associated component of some histone methyltransferase complexes which regulates transcription through recruitment of those complexes to gene promoters (PubMed : 19131338). Component of the Set1/Ash2 histone methyltransferase (HMT) complex, a complex that specifically methylates 'Lys-4' of histone H3, but not if the neighboring 'Lys-9' residue is already methylated (PubMed : 19556245). As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3 (PubMed : 19556245). May play a role in hematopoiesis (PubMed : 12670868). In association with RBBP5 and WDR5, stimulates the histone methyltransferase activities of KMT2A, KMT2B, KMT2C, KMT2D, SETD1A and SETD1B (PubMed : 21220120, PubMed : 22266653).

See full target information ASH2L

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 8:829 PubMed40442479

2025

RBAP48 facilitates the oral squamous cell carcinoma process in an androgen receptor-dependent and independent manners.

Applications

Unspecified application

Species

Unspecified reactive species

Xue Wang,Guangqi Yan,Hao Li,Chunyu Wang,Ye Kang,Shengli Wang,Wei Liu,Lin Lin,Renlong Zou,Kai Zeng,Manlin Wang,Ruina Luan,Baosheng Zhou,Yu Bai,Dongjun Yang,Bolin Ning,Ge Sun,Yue Zhao

Cancer cell international 25:49 PubMed39962568

2025

Comprehensive network pharmacology and experimental study to investigate the effect and mechanism of solasonine on breast carcinoma treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Wenkai Ge,Min Gao,Yingqi Dai,Gang Zheng,Li Yang,Wenshu Zuo,Xingsong Tian

OncoTargets and therapy 13:381-387 PubMed32021283

2020

ASH2L-Promoted Gene Expression Plays a Role in Mixed Lineage Leukemia-Rearranged Acute Leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Jie Wu,Li-Xia Li,Lu Liu,Si-Shu Zhao,Hai-Rong Qiu,Hui Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ASH2L antibody [EPR13106]

Flow Cytometry (Intracellular) - Anti-ASH2L antibody [EPR13106] (AB181117)

Immunocytochemistry/ Immunofluorescence - Anti-ASH2L antibody [EPR13106] (AB181117)

Immunoprecipitation - Anti-ASH2L antibody [EPR13106] (AB181117)

Western blot - Anti-ASH2L antibody [EPR13106] (AB181117)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise