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AB250358

Anti-ASH2L antibody [EPR13106] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal ASH2L antibody. Carrier free. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Human, Rat samples. Cited in 1 publication.

View Alternative Names

ASH2L1, ASH2L, Set1/Ash2 histone methyltransferase complex subunit ASH2, ASH2-like protein

4 Images
Flow Cytometry (Intracellular) - Anti-ASH2L antibody [EPR13106] - BSA and Azide free (AB250358)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-ASH2L antibody [EPR13106] - BSA and Azide free (AB250358)

This data was developed using ab181117, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of 2% paraformaldehyde fixed HeLa cells labeling ASH2L with ab181117 at 1/20 followed by Goat anti rabbit IgG (FITC) at 1/150 (pink). Rabbit monoclonal IgG was used as Isotype control (green).

Immunocytochemistry/ Immunofluorescence - Anti-ASH2L antibody [EPR13106] - BSA and Azide free (AB250358)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ASH2L antibody [EPR13106] - BSA and Azide free (AB250358)

This data was developed using ab181117, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of acetone fixed HeLa cells labeling ASH2L with ab181117 at 1/100 (green). Staining with Dapi is shown in blue.

Immunoprecipitation - Anti-ASH2L antibody [EPR13106] - BSA and Azide free (AB250358)
  • IP

Supplier Data

Immunoprecipitation - Anti-ASH2L antibody [EPR13106] - BSA and Azide free (AB250358)

This data was developed using ab181117, the same antibody clone in a different buffer formulation.

Immunoprecipitation analysis of 293T cells labeling ASH2L using ab181117 at 1/60 dilution. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/2000 was used as secondary antibody.

All lanes:

Immunoprecipitation - Anti-ASH2L antibody [EPR13106] (<a href='/en-us/products/primary-antibodies/ash2l-antibody-epr13106-ab181117'>ab181117</a>)

Predicted band size: 69 kDa

Observed band size: 50 kDa,80 kDa

false

Western blot - Anti-ASH2L antibody [EPR13106] - BSA and Azide free (AB250358)
  • WB

Supplier Data

Western blot - Anti-ASH2L antibody [EPR13106] - BSA and Azide free (AB250358)

This data was developed using ab181117, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ASH2L antibody [EPR13106] (<a href='/en-us/products/primary-antibodies/ash2l-antibody-epr13106-ab181117'>ab181117</a>) at 1/10000 dilution

Lane 1:

293T cell lysate at 20 µg

Lane 2:

K562 cell lysate at 20 µg

Lane 3:

C6 cell lysate at 20 µg

Lane 4:

PC-12 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 69 kDa

Observed band size: 80 kDa

false

  • Unconjugated

    Anti-ASH2L antibody [EPR13106]

  • HRP

    HRP Anti-ASH2L antibody [EPR13106]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13106

Isotype

IgG

Carrier free

Yes

Reacts with

Rat, Human

Applications

WB, Flow Cyt (Intra), IP, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

ab250358 is the carrier-free version of ab181117.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ASH2L also known as ASH2 is a protein with a mass of approximately 75 kDa. It functions as part of the trithorax group (TrxG) complex specifically the COMPASS-like complex where it plays an important role in histone methylation. This methylation occurs at histone H3 on lysine 4 (H3K4) an important modification for transcriptional activation. ASH2L expresses in various tissues with significant levels in the nucleus due to its involvement in chromatin modification.
Biological function summary

ASH2L participates in the regulation of gene expression. It acts as a core subunit of the SET/MLL family of H3K4 methyltransferases which form part of larger COMPASS-like complexes. These complexes modulate chromatin structure influencing transcriptional activation by methylating histone H3 at lysine 4. This modification is essential for the transition from transcriptional repression to activation during development and cell differentiation. ASH2L's role in these processes highlights its importance in maintaining proper gene expression patterns.

Pathways

ASH2L participates in the Wnt signaling pathway and influences the cell cycle. In the Wnt pathway ASH2L interacts with proteins such as β-catenin to modulate the transcription of target genes. It also affects the methylation status of promoters in various signaling pathways contributing to the regulation of gene expression involved in cell proliferation and differentiation. Additionally ASH2L is functionally connected to other proteins like MLL1 which shares involvement in chromatin remodeling and is important for epigenetic regulation.

ASH2L association with cancer and neurodevelopmental disorders is significant. Changes in ASH2L expression or function can lead to dysregulation of gene expression promoting oncogenesis or developmental abnormalities. In some cancers ASH2L's interaction with MLL can result in altered histone marks affecting tumor suppressor or oncogene expression. Besides cancer neurodevelopmental disorders like intellectual disability may have links to ASH2L due to its role in regulating genes involved in brain development. Understanding ASH2L's interactions and dysfunctions in these contexts provides insight into potential therapeutic approaches.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcriptional regulator (PubMed : 12670868). Component or associated component of some histone methyltransferase complexes which regulates transcription through recruitment of those complexes to gene promoters (PubMed : 19131338). Component of the Set1/Ash2 histone methyltransferase (HMT) complex, a complex that specifically methylates 'Lys-4' of histone H3, but not if the neighboring 'Lys-9' residue is already methylated (PubMed : 19556245). As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3 (PubMed : 19556245). May play a role in hematopoiesis (PubMed : 12670868). In association with RBBP5 and WDR5, stimulates the histone methyltransferase activities of KMT2A, KMT2B, KMT2C, KMT2D, SETD1A and SETD1B (PubMed : 21220120, PubMed : 22266653).
See full target information ASH2L
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Evidence-based complementary and alternative medicine : eCAM 2022:7757833 PubMed36091585

2022

Esculentoside A Alleviates Intestinal Dysmotility in Ulcerative Colitis by Regulating HS/CSE and NO/nNOS Systems.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Liu,Wenhua Wei,Shiwei Liang,Haicheng Fang,Jie Cao
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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