Rabbit Recombinant Monoclonal ASH2L antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Human, Rat samples.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-ASH2L antibody [EPR13107(B)] - BSA and Azide free (AB240191), expandable thumbnail](https://content.abcam.com/products/images/ash2l-antibody-epr13107b-bsa-and-azide-free-ab240191--immunocytochemistry-immunofluorescence-img20052.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-ASH2L antibody [EPR13107(B)] - BSA and Azide free (AB240191)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASH2L antibody [EPR13107(B)] - BSA and Azide free (AB240191), expandable thumbnail](https://content.abcam.com/products/images/ash2l-antibody-epr13107b-bsa-and-azide-free-ab240191--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img17575.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASH2L antibody [EPR13107(B)] - BSA and Azide free (AB240191)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASH2L antibody [EPR13107(B)] - BSA and Azide free (AB240191), expandable thumbnail](https://content.abcam.com/products/images/ash2l-antibody-epr13107b-bsa-and-azide-free-ab240191--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img100046.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASH2L antibody [EPR13107(B)] - BSA and Azide free (AB240191)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASH2L antibody [EPR13107(B)] - BSA and Azide free (AB240191), expandable thumbnail](https://content.abcam.com/products/images/ash2l-antibody-epr13107b-bsa-and-azide-free-ab240191--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img28086.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASH2L antibody [EPR13107(B)] - BSA and Azide free (AB240191)")
![Immunocytochemistry/ Immunofluorescence - Anti-ASH2L antibody [EPR13107(B)] - BSA and Azide free (AB240191), expandable thumbnail](https://content.abcam.com/products/images/ash2l-antibody-epr13107b-bsa-and-azide-free-ab240191--immunocytochemistry-immunofluorescence-img41763.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-ASH2L antibody [EPR13107(B)] - BSA and Azide free (AB240191)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Expected | Not recommended | Expected | Tested | Tested |
| Mouse | Tested | Not recommended | Expected | Expected | Expected |
| Rat | Predicted | Not recommended | Expected | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Transcriptional regulator (PubMed:12670868). Component or associated component of some histone methyltransferase complexes which regulates transcription through recruitment of those complexes to gene promoters (PubMed:19131338). Component of the Set1/Ash2 histone methyltransferase (HMT) complex, a complex that specifically methylates 'Lys-4' of histone H3, but not if the neighboring 'Lys-9' residue is already methylated (PubMed:19556245). As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3 (PubMed:19556245). May play a role in hematopoiesis (PubMed:12670868). In association with RBBP5 and WDR5, stimulates the histone methyltransferase activities of KMT2A, KMT2B, KMT2C, KMT2D, SETD1A and SETD1B (PubMed:21220120, PubMed:22266653).
Alternative names
ASH2L1, ASH2L, Set1/Ash2 histone methyltransferase complex subunit ASH2, ASH2-like protein
Recommended products
Rabbit Recombinant Monoclonal ASH2L antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Human, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR13107(B)
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab240191 is the carrier-free version of Anti-ASH2L antibody [EPR13107(B)] - Nuclear Marker ab176334.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ASH2L also known as ASH2 is a protein with a mass of approximately 75 kDa. It functions as part of the trithorax group (TrxG) complex specifically the COMPASS-like complex where it plays an important role in histone methylation. This methylation occurs at histone H3 on lysine 4 (H3K4) an important modification for transcriptional activation. ASH2L expresses in various tissues with significant levels in the nucleus due to its involvement in chromatin modification.
- Biological function summary
ASH2L participates in the regulation of gene expression. It acts as a core subunit of the SET/MLL family of H3K4 methyltransferases which form part of larger COMPASS-like complexes. These complexes modulate chromatin structure influencing transcriptional activation by methylating histone H3 at lysine 4. This modification is essential for the transition from transcriptional repression to activation during development and cell differentiation. ASH2L's role in these processes highlights its importance in maintaining proper gene expression patterns.
- Pathways
ASH2L participates in the Wnt signaling pathway and influences the cell cycle. In the Wnt pathway ASH2L interacts with proteins such as β-catenin to modulate the transcription of target genes. It also affects the methylation status of promoters in various signaling pathways contributing to the regulation of gene expression involved in cell proliferation and differentiation. Additionally ASH2L is functionally connected to other proteins like MLL1 which shares involvement in chromatin remodeling and is important for epigenetic regulation.
- Associated diseases and disorders
ASH2L association with cancer and neurodevelopmental disorders is significant. Changes in ASH2L expression or function can lead to dysregulation of gene expression promoting oncogenesis or developmental abnormalities. In some cancers ASH2L's interaction with MLL can result in altered histone marks affecting tumor suppressor or oncogene expression. Besides cancer neurodevelopmental disorders like intellectual disability may have links to ASH2L due to its role in regulating genes involved in brain development. Understanding ASH2L's interactions and dysfunctions in these contexts provides insight into potential therapeutic approaches.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
![Immunocytochemistry/ Immunofluorescence - Anti-ASH2L antibody [EPR13107(B)] - BSA and Azide free (ab240191), expandable thumbnail](https://content.abcam.com/products/images/ash2l-antibody-epr13107b-bsa-and-azide-free-ab240191--immunocytochemistry-immunofluorescence-img20052.jpg "Immunocytochemistry/ Immunofluorescence - Anti-ASH2L antibody [EPR13107(B)] - BSA and Azide free (ab240191)")
Immunocytochemistry/ Immunofluorescence - Anti-ASH2L antibody [EPR13107(B)] - BSA and Azide free (ab240191)
Immunocytochemistry/ Immunofluorescence analysis of 293T (Human embryonic kidney epithelial cell) cells labeling ASH2L with purified Anti-ASH2L antibody [EPR13107(B)] - Nuclear Marker ab176334 at 1/50 dilution (7.78 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ASH2L antibody [EPR13107(B)] - Nuclear Marker ab176334).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASH2L antibody [EPR13107(B)] - BSA and Azide free (ab240191), expandable thumbnail](https://content.abcam.com/products/images/ash2l-antibody-epr13107b-bsa-and-azide-free-ab240191--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img17575.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASH2L antibody [EPR13107(B)] - BSA and Azide free (ab240191)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASH2L antibody [EPR13107(B)] - BSA and Azide free (ab240191)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cerebrum tissue sections labeling ASH2L with purified Anti-ASH2L antibody [EPR13107(B)] - Nuclear Marker ab176334 at 1/8000 dilution (0.05 μg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ASH2L antibody [EPR13107(B)] - Nuclear Marker ab176334).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASH2L antibody [EPR13107(B)] - BSA and Azide free (ab240191), expandable thumbnail](https://content.abcam.com/products/images/ash2l-antibody-epr13107b-bsa-and-azide-free-ab240191--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img100046.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASH2L antibody [EPR13107(B)] - BSA and Azide free (ab240191)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASH2L antibody [EPR13107(B)] - BSA and Azide free (ab240191)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue sections labeling ASH2L with purified Anti-ASH2L antibody [EPR13107(B)] - Nuclear Marker ab176334 at 1/8000 dilution (0.05 μg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ASH2L antibody [EPR13107(B)] - Nuclear Marker ab176334).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASH2L antibody [EPR13107(B)] - BSA and Azide free (ab240191), expandable thumbnail](https://content.abcam.com/products/images/ash2l-antibody-epr13107b-bsa-and-azide-free-ab240191--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img28086.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASH2L antibody [EPR13107(B)] - BSA and Azide free (ab240191)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASH2L antibody [EPR13107(B)] - BSA and Azide free (ab240191)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast tissue sections labeling ASH2L with purified Anti-ASH2L antibody [EPR13107(B)] - Nuclear Marker ab176334 at 1/8000 dilution (0.05 μg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ASH2L antibody [EPR13107(B)] - Nuclear Marker ab176334).
![Immunocytochemistry/ Immunofluorescence - Anti-ASH2L antibody [EPR13107(B)] - BSA and Azide free (ab240191), expandable thumbnail](https://content.abcam.com/products/images/ash2l-antibody-epr13107b-bsa-and-azide-free-ab240191--immunocytochemistry-immunofluorescence-img41763.jpg "Immunocytochemistry/ Immunofluorescence - Anti-ASH2L antibody [EPR13107(B)] - BSA and Azide free (ab240191)")
Immunocytochemistry/ Immunofluorescence - Anti-ASH2L antibody [EPR13107(B)] - BSA and Azide free (ab240191)
Immunofluorescent analysis of HeLa labeling ASH2Lwith Anti-ASH2L antibody [EPR13107(B)] - Nuclear Marker ab176334 at 1/100 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ASH2L antibody [EPR13107(B)] - Nuclear Marker ab176334).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASH2L antibody [EPR13107(B)] - BSA and Azide free (ab240191), expandable thumbnail](https://content.abcam.com/products/images/ash2l-antibody-epr13107b-bsa-and-azide-free-ab240191--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img111934.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASH2L antibody [EPR13107(B)] - BSA and Azide free (ab240191)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASH2L antibody [EPR13107(B)] - BSA and Azide free (ab240191)
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeing ASH2L with Anti-ASH2L antibody [EPR13107(B)] - Nuclear Marker ab176334 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ASH2L antibody [EPR13107(B)] - Nuclear Marker ab176334).![Flow Cytometry (Intracellular) - Anti-ASH2L antibody [EPR13107(B)] - BSA and Azide free (ab240191), expandable thumbnail](https://content.abcam.com/products/images/ash2l-antibody-epr13107b-bsa-and-azide-free-ab240191--flow-cytometry-intracellular-img6310.jpg "Flow Cytometry (Intracellular) - Anti-ASH2L antibody [EPR13107(B)] - BSA and Azide free (ab240191)")
Flow Cytometry (Intracellular) - Anti-ASH2L antibody [EPR13107(B)] - BSA and Azide free (ab240191)
Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling ASH2L (red) with Anti-ASH2L antibody [EPR13107(B)] - Nuclear Marker ab176334 at a 1/1200 dilution. Cells were fixed with 80% methanol and permeabilized with 0.1% Tween-20. A goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ASH2L antibody [EPR13107(B)] - Nuclear Marker ab176334).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASH2L antibody [EPR13107(B)] - BSA and Azide free (ab240191), expandable thumbnail](https://content.abcam.com/products/images/ash2l-antibody-epr13107b-bsa-and-azide-free-ab240191--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img85275.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASH2L antibody [EPR13107(B)] - BSA and Azide free (ab240191)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASH2L antibody [EPR13107(B)] - BSA and Azide free (ab240191)
Immunohistochemical analysis of paraffin-embedded Human testis tissue labeing ASH2L with Anti-ASH2L antibody [EPR13107(B)] - Nuclear Marker ab176334 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ASH2L antibody [EPR13107(B)] - Nuclear Marker ab176334).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASH2L antibody [EPR13107(B)] - BSA and Azide free (ab240191), expandable thumbnail](https://content.abcam.com/products/images/ash2l-antibody-epr13107b-bsa-and-azide-free-ab240191--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img111934.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASH2L antibody [EPR13107(B)] - BSA and Azide free (ab240191)")
![Flow Cytometry (Intracellular) - Anti-ASH2L antibody [EPR13107(B)] - BSA and Azide free (ab240191), expandable thumbnail](https://content.abcam.com/products/images/ash2l-antibody-epr13107b-bsa-and-azide-free-ab240191--flow-cytometry-intracellular-img6310.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-ASH2L antibody [EPR13107(B)] - BSA and Azide free (ab240191)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASH2L antibody [EPR13107(B)] - BSA and Azide free (ab240191), expandable thumbnail](https://content.abcam.com/products/images/ash2l-antibody-epr13107b-bsa-and-azide-free-ab240191--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img85275.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASH2L antibody [EPR13107(B)] - BSA and Azide free (ab240191)")
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com