AB127896

Anti-Asialoglycoprotein Receptor 1/HL-1 antibody

Name: Anti-Asialoglycoprotein Receptor 1/HL-1 antibody (ab127896) | Abcam
Brand: Abcam Limited
SKU: AB127896
Price: 430 USD
Availability: InStock
Rating: 4 (3 reviews)

(3 Reviews)

(4 Publications)

Rabbit Polyclonal Asialoglycoprotein Receptor 1/HL-1 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ASGR1 aa 1-250.

View Alternative Names

CLEC4H1, ASGR1, Asialoglycoprotein receptor 1, ASGP-R 1, ASGPR 1, C-type lectin domain family 4 member H1, Hepatic lectin H1, HL-1

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody (AB127896)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody (AB127896)

HepG2 cells stained for ASGPR1 (green) using ab127896 at 1/500 dilution in ICC/IF. Counterstaining using Hoechst 33342 staining (blue).

Supplier Data

Western blot - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody (AB127896)

All lanes:

Western blot - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody (ab127896) at 1/3000 dilution

All lanes:

Mouse liver tissue extract at 50 µg

Predicted band size: 33 kDa

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Unknown

Western blot - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody (AB127896)

12% SDS Page

All lanes:

Western blot - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody (ab127896) at 1/1000 dilution

All lanes:

Mouse NIH/3T3 whole cell lysate at 30 µg

Predicted band size: 33 kDa

true

Unknown

Western blot - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody (AB127896)

12% SDS Page

All lanes:

Western blot - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody (ab127896) at 1/3000 dilution

All lanes:

HepG2 whole cell lysate at 30 µg

Predicted band size: 33 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human ASGR1 aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

P07306

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Asialoglycoprotein Receptor 1 (ASGR1) also known as ASGPR 1 or asialoglycoprotein receptor is a well-known protein with a critical role in the mechanism of recognizing and binding to asialoglycoproteins. It has a molecular mass of approximately 46 kDa. ASGR1 is expressed mostly in liver cells specifically hepatocytes. The unique characteristic of this receptor is its lectin-like nature which allows it to bind to specific sugar moieties present on desialylated glycoproteins. Through receptor-mediated endocytosis it facilitates their removal from the bloodstream.

Biological function summary

The function of ASGR1 extends significantly. It operates as part of the asialoglycoprotein receptor complex interfacing directly with the ligand proteins to mediate endocytosis. This process is essential in most mammalian systems for maintaining serum glycoprotein homeostasis. By clearing desialylated glycoproteins from circulation ASGR1 helps prevent potential adverse effects these molecules might cause like immune activation or interference with other biological functions.

Pathways

ASGR1 is integral to glycoprotein clearance pathways. It actively participates in the hepatic clearance pathway ensuring the regulation of serum glycoprotein levels. The removal of asialoglycoproteins involves interactions with several other hepatic proteins such as GalNAc transferase which can further facilitate the remodeling of glycoconjugates. Understanding these interactions is key for comprehending how the liver maintains overall metabolic balance.

ASGR1 is linked to certain liver-related conditions. Dysfunction or aberrant expression of the ASGR1 receptor can relate to elevated levels of serum glycoproteins which might be observed in liver cirrhosis. Elevated glycoproteins can exacerbate the progression of liver diseases. Furthermore ASGR1 protein interaction with viral glycoproteins has implications in hepatitis infections where it may inadvertently facilitate viral entry into hepatocytes affecting liver function and contributing to disease pathology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Mediates the endocytosis of plasma glycoproteins to which the terminal sialic acid residue on their complex carbohydrate moieties has been removed. The receptor recognizes terminal galactose and N-acetylgalactosamine units. After ligand binding to the receptor, the resulting complex is internalized and transported to a sorting organelle, where receptor and ligand are disassociated. The receptor then returns to the cell membrane surface.

See full target information ASGR1

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:15099 PubMed38956264

2024

Boiling histotripsy exhibits anti-fibrotic effects in animal models of liver fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Chanmin Joung,Jeongmin Heo,Ki Joo Pahk,Kisoo Pahk

Autophagy 20:1537-1558 PubMed38591519

2024

Alcohol-induced Golgiphagy is triggered by the downregulation of Golgi GTPase RAB3D.

Applications

Unspecified application

Species

Unspecified reactive species

Amanda J Macke,Taylor E Divita,Artem N Pachikov,Sundararajan Mahalingam,Ramesh Bellamkonda,Karuna Rasineni,Carol A Casey,Armen Petrosyan

Scientific reports 12:14462 PubMed36002564

2022

Investigation of the long-term healing response of the liver to boiling histotripsy treatment in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Jeongmin Heo,Chanmin Joung,Kisoo Pahk,Ki Joo Pahk

Molecular reproduction and development 86:1663-1670 PubMed31410930

2019

BRCA1 regulates HMGA2 levels in the Swan71 trophoblast cell line.

Applications

Unspecified application

Species

Unspecified reactive species

Rachel C West,Jennifer E Russ,Gerrit J Bouma,Quinton A Winger

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Asialoglycoprotein Receptor 1/HL-1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody (AB127896)

Western blot - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody (AB127896)

Western blot - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody (AB127896)

Western blot - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody (AB127896)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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