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Rabbit Recombinant Monoclonal Asialoglycoprotein Receptor 1/HL-1 antibody. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Human samples. Cited in 2 publications.


Images

Western blot - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22639-68] (AB254261), expandable thumbnail
  • Western blot - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22639-68] (AB254261), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22639-68] (AB254261), expandable thumbnail
  • Immunoprecipitation - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22639-68] (AB254261), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22639-68] (AB254261), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBICC/IFIHC-P
Human
Tested
Not recommended
Tested
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/30
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Tested
Tested

Species
Human
Dilution info
1/2000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

Mediates the endocytosis of plasma glycoproteins to which the terminal sialic acid residue on their complex carbohydrate moieties has been removed. The receptor recognizes terminal galactose and N-acetylgalactosamine units. After ligand binding to the receptor, the resulting complex is internalized and transported to a sorting organelle, where receptor and ligand are disassociated. The receptor then returns to the cell membrane surface.

Alternative names

CLEC4H1, ASGR1, Asialoglycoprotein receptor 1, ASGP-R 1, ASGPR 1, C-type lectin domain family 4 member H1, Hepatic lectin H1, HL-1

Recommended products

Rabbit Recombinant Monoclonal Asialoglycoprotein Receptor 1/HL-1 antibody. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Human samples. Cited in 2 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR22639-68
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The Asialoglycoprotein Receptor 1 (ASGR1) also known as ASGPR 1 or asialoglycoprotein receptor is a well-known protein with a critical role in the mechanism of recognizing and binding to asialoglycoproteins. It has a molecular mass of approximately 46 kDa. ASGR1 is expressed mostly in liver cells specifically hepatocytes. The unique characteristic of this receptor is its lectin-like nature which allows it to bind to specific sugar moieties present on desialylated glycoproteins. Through receptor-mediated endocytosis it facilitates their removal from the bloodstream.

Biological function summary

The function of ASGR1 extends significantly. It operates as part of the asialoglycoprotein receptor complex interfacing directly with the ligand proteins to mediate endocytosis. This process is essential in most mammalian systems for maintaining serum glycoprotein homeostasis. By clearing desialylated glycoproteins from circulation ASGR1 helps prevent potential adverse effects these molecules might cause like immune activation or interference with other biological functions.

Pathways

ASGR1 is integral to glycoprotein clearance pathways. It actively participates in the hepatic clearance pathway ensuring the regulation of serum glycoprotein levels. The removal of asialoglycoproteins involves interactions with several other hepatic proteins such as GalNAc transferase which can further facilitate the remodeling of glycoconjugates. Understanding these interactions is key for comprehending how the liver maintains overall metabolic balance.

Associated diseases and disorders

ASGR1 is linked to certain liver-related conditions. Dysfunction or aberrant expression of the ASGR1 receptor can relate to elevated levels of serum glycoproteins which might be observed in liver cirrhosis. Elevated glycoproteins can exacerbate the progression of liver diseases. Furthermore ASGR1 protein interaction with viral glycoproteins has implications in hepatitis infections where it may inadvertently facilitate viral entry into hepatocytes affecting liver function and contributing to disease pathology.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Western blot - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22639-68] (ab254261), expandable thumbnail

    Western blot - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22639-68] (ab254261)

    The diffuse bands between 40-50 kDa are likely due to glycosylation.

    The expression profile is consistent with what has been described in the literature (PMID:27241665, 29858079, 22898823).

    Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

    Exposure times: Lane 1: 5.5 seconds; Lane 2: 15 seconds.

    All lanes: Western blot - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22639-68] (ab254261) at 1/1000 dilution

    Lane 1: HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: Human liver tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 33 kDa

    Observed band size: 40-50 kDa

  • Western blot - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22639-68] (ab254261), expandable thumbnail

    Western blot - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22639-68] (ab254261)

    The diffuse bands between 40-50 kDa are likely due to glycosylation.

    The expression profile is consistent with what has been described in the literature (PMID:27241665, 29858079).

    This blot was developed using a higher sensitivity ECL substrate.

    Blocking/Diluting buffer and concentration: 5% NFDM/TBST

    All lanes: Western blot - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22639-68] (ab254261) at 1/1000 dilution

    All lanes: Huh7 (human hepatocellular carcinoma epithelial cell), whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 33 kDa

    Observed band size: 40-50 kDa

    Exposure time: 3min

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22639-68] (ab254261), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22639-68] (ab254261)

    Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Asialoglycoprotein Receptor 1/HL-1 with ab254261 at 1/2000 dilution (0.25 μg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in human liver (PMID:23979840,26422581,27241665). Counterstained with hematoxylin. Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

  • Immunoprecipitation - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22639-68] (ab254261), expandable thumbnail

    Immunoprecipitation - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22639-68] (ab254261)

    Asialoglycoprotein Receptor 1/HL-1 was immunoprecipitated from 0.35 mg human liver lysate with ab254261 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab254261 at 1/1000 dilution (0.45 μg/ml). VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: Human liver lysate 10μg.

    Lane 2: ab254261 IP in human liver lysate.

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab254261 in human liver lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second

    All lanes: Immunoprecipitation - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22639-68] (ab254261)

    Predicted band size: 33 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22639-68] (ab254261), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22639-68] (ab254261)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue labeling Asialoglycoprotein Receptor 1/HL-1 with ab254261 at 1/2000 dilution (0.25 μg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in human liver cancer (PMID:23979840,26422581,27241665).No staining in human colon (PMID:23979840). Counterstained with hematoxylin. Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22639-68] (ab254261), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22639-68] (ab254261)

    Negative control: No staining in human colon (PMID:23979840).

    Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Asialoglycoprotein Receptor 1/HL-1 with ab254261 at 1/2000 dilution (0.25 μg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Counterstained with hematoxylin. Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

  • Immunoprecipitation - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22639-68] (ab254261), expandable thumbnail

    Immunoprecipitation - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22639-68] (ab254261)

    Asialoglycoprotein Receptor 1/HL-1 was immunoprecipitated from 0.35 mg HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate with ab254261 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab254261 1/1000 dilution (0.45 μg/ml). VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate 10μg
    Lane 2: ab254261 IP in HepG2 whole cell lysate
    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab254261 in HepG2 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second

    All lanes: Immunoprecipitation - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22639-68] (ab254261)

    Predicted band size: 33 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22639-68] (ab254261), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22639-68] (ab254261)

    Immunofluorescent analysis of 100% Methanol-fixed HepG2 (human hepatocellular carcinoma epithelial cell) cells labeling Asialoglycoprotein Receptor 1/HL-1 with at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in HepG2 cell line is observed. Methanol fixation is preferred.

    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

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Product protocols

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