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Rabbit Recombinant Monoclonal Asialoglycoprotein Receptor 1/HL-1 antibody. Carrier free. Suitable for IP, Flow Cyt, WB, IHC-P, ICC/IF and reacts with Human samples.

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Images

Immunoprecipitation - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22642-17] - BSA and Azide free (AB256509), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22642-17] - BSA and Azide free (AB256509), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22642-17] - BSA and Azide free (AB256509), expandable thumbnail
  • Flow Cytometry - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22642-17] - BSA and Azide free (AB256509), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22642-17] - BSA and Azide free (AB256509), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBIHC-PICC/IF
Human
Tested
Tested
Expected
Tested
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

Mediates the endocytosis of plasma glycoproteins to which the terminal sialic acid residue on their complex carbohydrate moieties has been removed. The receptor recognizes terminal galactose and N-acetylgalactosamine units. After ligand binding to the receptor, the resulting complex is internalized and transported to a sorting organelle, where receptor and ligand are disassociated. The receptor then returns to the cell membrane surface.

Alternative names

CLEC4H1, ASGR1, Asialoglycoprotein receptor 1, ASGP-R 1, ASGPR 1, C-type lectin domain family 4 member H1, Hepatic lectin H1, HL-1

Recommended products

Rabbit Recombinant Monoclonal Asialoglycoprotein Receptor 1/HL-1 antibody. Carrier free. Suitable for IP, Flow Cyt, WB, IHC-P, ICC/IF and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR22642-17
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab256509 is the carrier-free version of Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22642-17] ab254262.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The Asialoglycoprotein Receptor 1 (ASGR1) also known as ASGPR 1 or asialoglycoprotein receptor is a well-known protein with a critical role in the mechanism of recognizing and binding to asialoglycoproteins. It has a molecular mass of approximately 46 kDa. ASGR1 is expressed mostly in liver cells specifically hepatocytes. The unique characteristic of this receptor is its lectin-like nature which allows it to bind to specific sugar moieties present on desialylated glycoproteins. Through receptor-mediated endocytosis it facilitates their removal from the bloodstream.

Biological function summary

The function of ASGR1 extends significantly. It operates as part of the asialoglycoprotein receptor complex interfacing directly with the ligand proteins to mediate endocytosis. This process is essential in most mammalian systems for maintaining serum glycoprotein homeostasis. By clearing desialylated glycoproteins from circulation ASGR1 helps prevent potential adverse effects these molecules might cause like immune activation or interference with other biological functions.

Pathways

ASGR1 is integral to glycoprotein clearance pathways. It actively participates in the hepatic clearance pathway ensuring the regulation of serum glycoprotein levels. The removal of asialoglycoproteins involves interactions with several other hepatic proteins such as GalNAc transferase which can further facilitate the remodeling of glycoconjugates. Understanding these interactions is key for comprehending how the liver maintains overall metabolic balance.

Associated diseases and disorders

ASGR1 is linked to certain liver-related conditions. Dysfunction or aberrant expression of the ASGR1 receptor can relate to elevated levels of serum glycoproteins which might be observed in liver cirrhosis. Elevated glycoproteins can exacerbate the progression of liver diseases. Furthermore ASGR1 protein interaction with viral glycoproteins has implications in hepatitis infections where it may inadvertently facilitate viral entry into hepatocytes affecting liver function and contributing to disease pathology.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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