AB223769

Anti-Asialoglycoprotein Receptor 1/HL-1 antibody - N-terminal

Name: Anti-Asialoglycoprotein Receptor 1/HL-1 antibody - N-terminal (ab223769) | Abcam
Brand: Abcam Limited
SKU: AB223769
Price: 545 USD
Availability: InStock
Rating: 4 (4 reviews)

(4 Reviews)

(2 Publications)

Rabbit Polyclonal Asialoglycoprotein Receptor 1/HL-1 antibody. N-terminal. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ASGR1 aa 1-50.

View Alternative Names

CLEC4H1, ASGR1, Asialoglycoprotein receptor 1, ASGP-R 1, ASGPR 1, C-type lectin domain family 4 member H1, Hepatic lectin H1, HL-1

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody - N-terminal (AB223769)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody - N-terminal (AB223769)

Paraffin-embedded human lymph node tissue staining Asialoglycoprotein Receptor 1 with ab223769 at 1/500 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody - N-terminal (AB223769)

Paraffin-embedded human pancreas tissue staining Asialoglycoprotein Receptor 1 with ab223769 at 1/500 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody - N-terminal (AB223769)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody - N-terminal (AB223769)

PFA-fixed, Triton X-100 permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells stained for Asialoglycoprotein Receptor 1/HL-1 (green) using ab223769 at 4 μg/ml in ICC/IF.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody - N-terminal (AB223769)

Paraffin-embedded human liver tissue staining Asialoglycoprotein Receptor 1 with ab223769 at 1/500 dilution in immunohistochemical analysis. High expression shown.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody - N-terminal (AB223769)

Paraffin-embedded human colon tissue staining Asialoglycoprotein Receptor 1 with ab223769 at 1/500 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody - N-terminal (AB223769)

All lanes:

Western blot - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody - N-terminal (ab223769) at 1/100 dilution

All lanes:

HepG2 (human liver hepatocellular carcinoma cell line) cell lysate

Predicted band size: 33 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human ASGR1 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

P07306

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Asialoglycoprotein Receptor 1 (ASGR1) also known as ASGPR 1 or asialoglycoprotein receptor is a well-known protein with a critical role in the mechanism of recognizing and binding to asialoglycoproteins. It has a molecular mass of approximately 46 kDa. ASGR1 is expressed mostly in liver cells specifically hepatocytes. The unique characteristic of this receptor is its lectin-like nature which allows it to bind to specific sugar moieties present on desialylated glycoproteins. Through receptor-mediated endocytosis it facilitates their removal from the bloodstream.

Biological function summary

The function of ASGR1 extends significantly. It operates as part of the asialoglycoprotein receptor complex interfacing directly with the ligand proteins to mediate endocytosis. This process is essential in most mammalian systems for maintaining serum glycoprotein homeostasis. By clearing desialylated glycoproteins from circulation ASGR1 helps prevent potential adverse effects these molecules might cause like immune activation or interference with other biological functions.

Pathways

ASGR1 is integral to glycoprotein clearance pathways. It actively participates in the hepatic clearance pathway ensuring the regulation of serum glycoprotein levels. The removal of asialoglycoproteins involves interactions with several other hepatic proteins such as GalNAc transferase which can further facilitate the remodeling of glycoconjugates. Understanding these interactions is key for comprehending how the liver maintains overall metabolic balance.

ASGR1 is linked to certain liver-related conditions. Dysfunction or aberrant expression of the ASGR1 receptor can relate to elevated levels of serum glycoproteins which might be observed in liver cirrhosis. Elevated glycoproteins can exacerbate the progression of liver diseases. Furthermore ASGR1 protein interaction with viral glycoproteins has implications in hepatitis infections where it may inadvertently facilitate viral entry into hepatocytes affecting liver function and contributing to disease pathology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Mediates the endocytosis of plasma glycoproteins to which the terminal sialic acid residue on their complex carbohydrate moieties has been removed. The receptor recognizes terminal galactose and N-acetylgalactosamine units. After ligand binding to the receptor, the resulting complex is internalized and transported to a sorting organelle, where receptor and ligand are disassociated. The receptor then returns to the cell membrane surface.

See full target information ASGR1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Autophagy 20:1537-1558 PubMed38591519

2024

Alcohol-induced Golgiphagy is triggered by the downregulation of Golgi GTPase RAB3D.

Applications

Unspecified application

Species

Unspecified reactive species

Amanda J Macke,Taylor E Divita,Artem N Pachikov,Sundararajan Mahalingam,Ramesh Bellamkonda,Karuna Rasineni,Carol A Casey,Armen Petrosyan

Cell reports 42:112047 PubMed36724075

2023

RNA exosome ribonuclease DIS3 degrades Pou6f1 to promote mouse pre-implantation cell differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Di Wu,Jurrien Dean

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Asialoglycoprotein Receptor 1/HL-1 antibody - N-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody - N-terminal (AB223769)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody - N-terminal (AB223769)

Immunocytochemistry/ Immunofluorescence - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody - N-terminal (AB223769)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody - N-terminal (AB223769)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody - N-terminal (AB223769)

Western blot - Anti-Asialoglycoprotein Receptor 1/HL-1 antibody - N-terminal (AB223769)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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