Anti-ASIC1 antibody [EPR25411-161]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASIC1 antibody [EPR25411-161] (AB300563)

Immunohistochemical analysis of paraffin-embedded Panel A HEK-293T c tissue labeling ASIC1 with ab300563 at 1/5000 (0.1 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on (A) HEK-293T transfected with a human ASIC1 expression vector, no staining on (B) HEK-293T cells transfected with a human ASIC2a expression vector and (C) HEK-293T transfected with an empty vector. The section was incubated with ab300563 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASIC1 antibody [EPR25411-161] (AB300563)

Immunohistochemical analysis of paraffin-embedded Rat cardiac muscle tissue labeling ASIC1 with ab300563 at 1/1000 (0.498 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Negative control : no staining in rat cardiac muscle. The section was incubated with ab300563 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASIC1 antibody [EPR25411-161] (AB300563)

Immunohistochemical analysis of paraffin-embedded Rat dorsal root gang tissue labeling ASIC1 with ab300563 at 1/1000 (0.498 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining in rat dorsal root ganglion neurons. The section was incubated with ab300563 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASIC1 antibody [EPR25411-161] (AB300563)

Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling ASIC1 with ab300563 at 1/1000 (0.498 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Negative control : no staining in mouse cardiac muscle. The section was incubated with ab300563 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASIC1 antibody [EPR25411-161] (AB300563)

Immunohistochemical analysis of paraffin-embedded Mouse dorsal root ga tissue labeling ASIC1 with ab300563 at 1/1000 (0.498 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining in mouse dorsal root ganglion neurons. The section was incubated with ab300563 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IP

Supplier Data

Immunoprecipitation - Anti-ASIC1 antibody [EPR25411-161] (AB300563)

ASIC1 was immunoprecipitated from 0.35 mg Mouse cerebellum tissue lysate 10 ug with ab300563 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300563 at 1/1000 dilution.

VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-ASIC1 antibody [EPR25411-161] (ab300563) at 1/1000 dilution

Lane 1:

Mouse cerebellum tissue lysate at 10 µg

Lane 2:

ab300563 at 1/30 IP in Mouse cerebellum tissue lysate at 10 µg

Lane 3:

Rabbit monoclonal IgG (<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of ab300563 in mouse cerebellum tissue lysate at 10 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Observed band size: 70 kDa,150 kDa

false

Exposure time: 3min

• WB

Supplier Data

Western blot - Anti-ASIC1 antibody [EPR25411-161] (AB300563)

Blocking and diluting buffer and concentration : 5% NFDM/TBST The observed MW is consistent with what has been described in the literature (PMID : 19218436, PMID : 26252376).

All lanes:

Western blot - Anti-ASIC1 antibody [EPR25411-161] (ab300563) at 1/1000 dilution

Lane 1:

Rat brain tissue lysate

Lane 2:

Rat spinal cord tissue lysate

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 70 kDa,150 kDa

false

Exposure time: 3min

• WB

Supplier Data

Western blot - Anti-ASIC1 antibody [EPR25411-161] (AB300563)

Blocking and diluting buffer and concentration : 5% NFDM/TBST The observed MW is consistent with what has been described in the literature (PMID : 19218436, PMID : 26252376). Negative controls : heart, kidney (PMID : 9037075).

All lanes:

Western blot - Anti-ASIC1 antibody [EPR25411-161] (ab300563) at 1/1000 dilution

Lane 1:

Mouse cerebellum tissue lysate

Lane 2:

Mouse brain tissue lysate

Lane 3:

Mouse spinal cord tissue lysate

Lane 4:

Mouse heart tissue lysate

Lane 5:

Mouse kidney tissue lysate

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 70 kDa,150 kDa

false

Exposure time: 48s