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AB169768

Anti-ASIC2 antibody [EPR11029]

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(2 Publications)

Rabbit Recombinant Monoclonal ASIC2 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 2 publications.

View Alternative Names

ACCN, ACCN1, BNAC1, MDEG, ASIC2, Acid-sensing ion channel 2, Amiloride-sensitive brain sodium channel, Amiloride-sensitive cation channel neuronal 1, Brain sodium channel 1, Mammalian degenerin homolog, BNC1, BNaC1

2 Images
Western blot - Anti-ASIC2 antibody [EPR11029] (AB169768)
  • WB

Unknown

Western blot - Anti-ASIC2 antibody [EPR11029] (AB169768)

All lanes:

Western blot - Anti-ASIC2 antibody [EPR11029] (ab169768) at 1/1000 dilution

Lane 1:

Human fetal kidney lysate at 10 µg

Lane 2:

Human glioma lysate at 10 µg

Secondary

All lanes:

HRP labeled goat anti-rabbit at 1/2000 dilution

Predicted band size: 57 kDa

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Western blot - Anti-ASIC2 antibody [EPR11029] (AB169768)
  • WB

Supplier Data

Western blot - Anti-ASIC2 antibody [EPR11029] (AB169768)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-ASIC2 antibody [EPR11029] (ab169768) at 1/1000 dilution

Lane 1:

Mouse brain lysate

Lane 2:

Rat brain lysate

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>)

Predicted band size: 57 kDa

Observed band size: 58 kDa

false

  • Carrier free

    Anti-ASIC2 antibody [EPR11029] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11029

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ASIC2 also known as amiloride-sensitive cation channel 2 or ACCN1 is a protein involved in ion transport. It forms part of the acid-sensing ion channel (ASIC) family and functions as a voltage-insensitive proton-gated ion channel. ASIC2 is known to have a mass of approximately 60 kDa. Expression is mainly noted in the central nervous system particularly in neurons as well as in other tissues such as the heart and lungs.
Biological function summary

ASIC2 contributes to the detection and response to changes in extracellular pH playing a role in processes such as pain perception and synaptic plasticity. ASIC2 can form homo- or heteromeric complexes with other ASIC proteins influencing the channel properties like ion selectivity and gating. By participating in these complexes ASIC2 affects neuronal signaling and potentially mood regulation in the central nervous system.

Pathways

ASIC2 plays a role in the proton-activated pathways by influencing neuronal excitability. One key pathway includes the pain transduction pathway where ASIC2 in combination with other ASIC family members like ASIC1 and ASIC3 helps mediate pain sensation in response to acids. This regulation is important for maintaining normal sensory functions and could be linked with synaptic functions and neurodegenerative processes.

ASIC2 relates to conditions such as ischemic brain injury and anxiety disorders. During ischemic events ASIC2 contributes to neuronal death making it a target for potential therapeutic interventions. In anxiety disorders alterations in ASIC2 expression or function could impact mood regulation. The protein interacts with other proteins like ASIC1 in these disorder-related pathways suggesting that modulating ASIC interactions might offer a strategy for therapeutic developments.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. Also permeable for Li(+) and K(+). Generates a biphasic current with a fast inactivating and a slow sustained phase. Heteromeric channel assembly seems to modulate.
See full target information ASIC2
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Chinese medicine 16:66 PubMed34321072

2021

Pterostilbene alleviates pulmonary fibrosis by regulating ASIC2.

Applications

Unspecified application

Species

Unspecified reactive species

Yanfang Peng,Yingwen Zhang,Yabing Zhang,Xiuping Wang,Yukun Xia

Scientific reports 7:5870 PubMed28725010

2017

Glucose Deficiency Elevates Acid-Sensing Ion Channel 2a Expression and Increases Seizure Susceptibility in Temporal Lobe Epilepsy.

Applications

Unspecified application

Species

Unspecified reactive species

Haitao Zhang,Guodong Gao,Yu Zhang,Yang Sun,Huanfa Li,Shan Dong,Wei Ma,Bei Liu,Weiwen Wang,Hao Wu,Hua Zhang
View all publications
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