AB249515

Anti-ASIC2 antibody [EPR11029] - BSA and Azide free

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal ASIC2 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

ACCN, ACCN1, BNAC1, MDEG, ASIC2, Acid-sensing ion channel 2, Amiloride-sensitive brain sodium channel, Amiloride-sensitive cation channel neuronal 1, Brain sodium channel 1, Mammalian degenerin homolog, BNC1, BNaC1

2 Images

Supplier Data

Western blot - Anti-ASIC2 antibody [EPR11029] - BSA and Azide free (AB249515)

This data was developed using ab169768, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-ASIC2 antibody [EPR11029] (<a href='/en-us/products/primary-antibodies/asic2-antibody-epr11029-ab169768'>ab169768</a>) at 1/1000 dilution

Lane 1:

Mouse brain lysate

Lane 2:

Rat brain lysate

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>)

Predicted band size: 57 kDa

Observed band size: 58 kDa

false

Unknown

Western blot - Anti-ASIC2 antibody [EPR11029] - BSA and Azide free (AB249515)

This data was developed using ab169768, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ASIC2 antibody [EPR11029] (<a href='/en-us/products/primary-antibodies/asic2-antibody-epr11029-ab169768'>ab169768</a>) at 1/1000 dilution

Lane 1:

Human fetal kidney lysate at 10 µg

Lane 2:

Human glioma lysate at 10 µg

Secondary

All lanes:

HRP labeled goat anti-rabbit at 1/2000 dilution

Predicted band size: 57 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11029

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab249515 is the carrier-free version of ab169768.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ASIC2 also known as amiloride-sensitive cation channel 2 or ACCN1 is a protein involved in ion transport. It forms part of the acid-sensing ion channel (ASIC) family and functions as a voltage-insensitive proton-gated ion channel. ASIC2 is known to have a mass of approximately 60 kDa. Expression is mainly noted in the central nervous system particularly in neurons as well as in other tissues such as the heart and lungs.

Biological function summary

ASIC2 contributes to the detection and response to changes in extracellular pH playing a role in processes such as pain perception and synaptic plasticity. ASIC2 can form homo- or heteromeric complexes with other ASIC proteins influencing the channel properties like ion selectivity and gating. By participating in these complexes ASIC2 affects neuronal signaling and potentially mood regulation in the central nervous system.

Pathways

ASIC2 plays a role in the proton-activated pathways by influencing neuronal excitability. One key pathway includes the pain transduction pathway where ASIC2 in combination with other ASIC family members like ASIC1 and ASIC3 helps mediate pain sensation in response to acids. This regulation is important for maintaining normal sensory functions and could be linked with synaptic functions and neurodegenerative processes.

ASIC2 relates to conditions such as ischemic brain injury and anxiety disorders. During ischemic events ASIC2 contributes to neuronal death making it a target for potential therapeutic interventions. In anxiety disorders alterations in ASIC2 expression or function could impact mood regulation. The protein interacts with other proteins like ASIC1 in these disorder-related pathways suggesting that modulating ASIC interactions might offer a strategy for therapeutic developments.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. Also permeable for Li(+) and K(+). Generates a biphasic current with a fast inactivating and a slow sustained phase. Heteromeric channel assembly seems to modulate.

See full target information ASIC2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

mBio 15:e0259523 PubMed38095418

2023

Susceptibility of to autophagy in human cells relies on multiple interacting parasite loci.

Applications

Unspecified application

Species

Unspecified reactive species

Nicholas Rinkenberger,Alex Rosenberg,Joshua B Radke,Jaya Bhushan,Tadakimi Tomita,Louis M Weiss,L David Sibley

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ASIC2 antibody [EPR11029] - BSA and Azide free

Western blot - Anti-ASIC2 antibody [EPR11029] - BSA and Azide free (AB249515)

Western blot - Anti-ASIC2 antibody [EPR11029] - BSA and Azide free (AB249515)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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