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Rabbit Recombinant Monoclonal ASIC3 antibody. Carrier free. Suitable for IHC-Fr, WB and reacts with Rat, Mouse samples.

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Images

Western blot - Anti-ASIC3 antibody [EPR26557-87] - BSA and Azide free (AB305244), expandable thumbnail
  • Western blot - Anti-ASIC3 antibody [EPR26557-87] - BSA and Azide free (AB305244), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-ASIC3 antibody [EPR26557-87] - BSA and Azide free (AB305244), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-ASIC3 antibody [EPR26557-87] - BSA and Azide free (AB305244), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-ASIC3 antibody [EPR26557-87] - BSA and Azide free (AB305244), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPIHC-FrIHC-PWB
Human
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Not recommended
Tested
Not recommended
Tested
Rat
Not recommended
Tested
Not recommended
Tested

Not recommended
Not recommended

Species
Rat, Mouse, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Rat, Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Rat, Mouse, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Rat
Dilution info
-
Notes

Please load at least 40ug of lysate to improve the Western blot results.

Species
Mouse
Dilution info
-
Notes

Please load at least 40ug of lysate to improve the Western blot results.

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Target data

Function

Cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. Generates a biphasic current with a fast inactivating and a slow sustained phase. In sensory neurons is proposed to mediate the pain induced by acidosis that occurs in ischemic, damaged or inflamed tissue. May be involved in hyperalgesia. May play a role in mechanoreception. Heteromeric channel assembly seems to modulate channel properties.

Alternative names

Accn3, Drasic, Asic3, Acid-sensing ion channel 3, ASIC3, Amiloride-sensitive cation channel 3, Dorsal root ASIC, DRASIC

Recommended products

Rabbit Recombinant Monoclonal ASIC3 antibody. Carrier free. Suitable for IHC-Fr, WB and reacts with Rat, Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR26557-87
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

ASIC3 also known as Acid-Sensing Ion Channel 3 or DRASIC is a protein channel involved in detecting variations in pH. This protein has a molecular mass of approximately 56 kDa and falls within the ASIC family of proton-gated sodium channels. ASIC3 is widely expressed in peripheral sensory neurons particularly in dorsal root and trigeminal ganglia which are areas pivotal for detecting pain and mechanosensation. The localization suggests a specialized role in responding to acidic environments.

Biological function summary

ASIC3 functions through responding to changes in extracellular pH allowing sodium ions to enter cells when the pH decreases. It does not act solo and usually forms homomeric or heteromeric complexes with other ASIC subunits such as ASIC1 or ASIC2. This combination enhances its sensitivity and response diversity to varying acidic conditions contributing to processes like pain sensing and mechanotransduction. In muscle tissue ASIC3 plays a role in sensing metabolic changes that occur during intensive exercise.

Pathways

ASIC3 integrates into systems related to pain and mechanosensory pathways. It participates prominently in the nociceptive signaling pathway associated with the perception of pain induced by protons. ASIC3 works alongside proteins such as TRPV1 another ion channel known for its role in pain and thermal sensation. Interconnected with these pathways ASIC3 impacts physiological responses like hyperalgesia in acidic environments.

Associated diseases and disorders

ASIC3 significantly contributes to conditions such as chronic pain and acid-induced pain from tissue injury or inflammation. It associates with migraines and inflammatory pain magnifying pain sensations under acidic conditions. In these contexts ASIC3 collaborates with proteins like the NaV1.7 sodium channel further influencing nerve excitability and pain perception. Targeting ASIC3 could potentially alleviate symptoms associated with these pain disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-ASIC3 antibody [EPR26557-87] - BSA and Azide free (ab305244), expandable thumbnail

    Western blot - Anti-ASIC3 antibody [EPR26557-87] - BSA and Azide free (ab305244)

    ASIC3 Western blot staining using rabbit Anti-ASIC3 antibody

    This data was developed using Anti-ASIC3 antibody [EPR26557-80] ab302776, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    It is recommended to optimize experimental conditions by increasing the sample loading amount, using a lower antibody dilution ratio, and employing femtogram-level sensitivity substrates.

    Lanes 1 - 4: Western blot - Anti-ASIC3 antibody [EPR26557-80] (Anti-ASIC3 antibody [EPR26557-80] ab302776) at 1/1000 dilution

    Lanes 5 - 8: Western blot - Anti-ASIC3 antibody [EPR26557-87] (Anti-ASIC3 antibody [EPR26557-87] ab305243) at 1/1000 dilution

    Lanes 1 and 5: Mouse dorsal ganglion tissue lysate at 20 µg

    Lanes 2 and 6: Rat dorsal ganglion tissue lysate at 20 µg

    Lanes 3 and 7: Mouse dorsal ganglion tissue lysate at 40 µg

    Lanes 4 and 8: Rat dorsal ganglion tissue lysate at 40 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 58 kDa

    Observed band size: 60 kDa

    Exposure time: 180s

  • Western blot - Anti-ASIC3 antibody [EPR26557-87] - BSA and Azide free (ab305244), expandable thumbnail

    Western blot - Anti-ASIC3 antibody [EPR26557-87] - BSA and Azide free (ab305244)

    This data was developed using 305243, the same antibody clone in a different buffer formulation.
    Blocking and diluting buffer and concentration: Negative controls: kidney, lung (PMID:11872753).

    Samples are non-boiled as boiling may cause protein aggregates.
    26 seconds
    Exposure time:

    All lanes: Western blot - Anti-ASIC3 antibody [EPR26557-87] (Anti-ASIC3 antibody [EPR26557-87] ab305243) at 1/1000 dilution

    Lane 1: Rat dorsal galion tissue lysate 20 μg

    Lane 2: Rat lung tissue lysate 20 μg

    Lane 3: Rat kidney tissue lysate 20 μg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 58 kDa

    Exposure time: 26s

  • Immunohistochemistry (Frozen sections) - Anti-ASIC3 antibody [EPR26557-87] - BSA and Azide free (ab305244), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-ASIC3 antibody [EPR26557-87] - BSA and Azide free (ab305244)

    This data was developed using Anti-ASIC3 antibody [EPR26557-87] ab305243, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeABilized frozen Rat heart (fresh) tissue lABeling Acid-sensing ion channel 3 with Anti-ASIC3 antibody [EPR26557-87] ab305243 at 1/100 (4.76 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Negative control: confocal image showing no staining on rat heart (PMID:?9707631). The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-ASIC3 antibody [EPR26557-87] ab305243 for 60 mins at room temperature. The section was then mounted using Fluoromount?.The immunostaining was performed on a Leica Biosystems BOND? RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbedat 1/1000 2 ug/mL dilution.

  • Immunohistochemistry (Frozen sections) - Anti-ASIC3 antibody [EPR26557-87] - BSA and Azide free (ab305244), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-ASIC3 antibody [EPR26557-87] - BSA and Azide free (ab305244)

    This data was developed using Anti-ASIC3 antibody [EPR26557-87] ab305243, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeABilized frozen Rat dorsal root ganglion (fresh) tissue lABeling Acid-sensing ion channel 3 with Anti-ASIC3 antibody [EPR26557-87] ab305243 at 1/100 (4.76 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Confocal image showing positive staining on rat dorsal ganglion. The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-ASIC3 antibody [EPR26557-87] ab305243 for 60 mins at room temperature. The section was then mounted using Fluoromount?.The immunostaining was performed on a Leica Biosystems BOND? RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbedat 1/1000 2 ug/mL dilution.

  • Immunohistochemistry (Frozen sections) - Anti-ASIC3 antibody [EPR26557-87] - BSA and Azide free (ab305244), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-ASIC3 antibody [EPR26557-87] - BSA and Azide free (ab305244)

    This data was developed using Anti-ASIC3 antibody [EPR26557-87] ab305243, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeABilized frozen Mouse heart (fresh) tissue lABeling Acid-sensing ion channel 3 with Anti-ASIC3 antibody [EPR26557-87] ab305243 at 1/100 (4.76 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Negative control: confocal image showing no staining on mouse heart (PMID:?9707631). The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-ASIC3 antibody [EPR26557-87] ab305243 for 60 mins at room temperature. The section was then mounted using Fluoromount?.The immunostaining was performed on a Leica Biosystems BOND? RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbedat 1/1000 2 ug/mL dilution.

  • Immunohistochemistry (Frozen sections) - Anti-ASIC3 antibody [EPR26557-87] - BSA and Azide free (ab305244), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-ASIC3 antibody [EPR26557-87] - BSA and Azide free (ab305244)

    This data was developed using Anti-ASIC3 antibody [EPR26557-87] ab305243, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeABilized frozen Mouse dorsal root ganglion (fresh) tissue lABeling Acid-sensing ion channel 3 with Anti-ASIC3 antibody [EPR26557-87] ab305243 at 1/100 (4.76 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Confocal image showing positive staining on mouse dorsal root ganglion. The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-ASIC3 antibody [EPR26557-87] ab305243 for 60 mins at room temperature. The section was then mounted using Fluoromount?.The immunostaining was performed on a Leica Biosystems BOND? RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-RABbit IgG H&L (Alexa Fluor? 488) preadsorbedat 1/1000 2 ug/mL dilution.

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