Rabbit Polyclonal ASK1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human MAP3K5 aa 950 to C-terminus.
Images
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human MAP3K5 aa 950 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q99683
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Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Cow | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Cow | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Cow | Dilution info - | Notes - |
Associated Products
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3 products for Alternative Product
Target data
Function
Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Mediates signaling for determination of cell fate such as differentiation and survival. Plays a crucial role in the apoptosis signal transduction pathway through mitochondria-dependent caspase activation. MAP3K5/ASK1 is required for the innate immune response, which is essential for host defense against a wide range of pathogens. Mediates signal transduction of various stressors like oxidative stress as well as by receptor-mediated inflammatory signals, such as the tumor necrosis factor (TNF) or lipopolysaccharide (LPS). Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade and the p38 MAPK signal transduction cascade through the phosphorylation and activation of several MAP kinase kinases like MAP2K4/SEK1, MAP2K3/MKK3, MAP2K6/MKK6 and MAP2K7/MKK7. These MAP2Ks in turn activate p38 MAPKs and c-jun N-terminal kinases (JNKs). Both p38 MAPK and JNKs control the transcription factors activator protein-1 (AP-1).
Alternative names
Mitogen-activated protein kinase kinase kinase 5, Apoptosis signal-regulating kinase 1, MAPK/ERK kinase kinase 5, ASK-1, MEK kinase 5, MEKK 5, MAP3K5, ASK1, MAPKKK5, MEKK5
Recommended products
Rabbit Polyclonal ASK1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human MAP3K5 aa 950 to C-terminus.
Alternative names
Mitogen-activated protein kinase kinase kinase 5, Apoptosis signal-regulating kinase 1, MAPK/ERK kinase kinase 5, ASK-1, MEK kinase 5, MEKK 5, MAP3K5, ASK1, MAPKKK5, MEKK5
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human MAP3K5 aa 950 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q99683
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The ASK1 protein also known as apoptosis signal-regulating kinase 1 is an important component in cellular stress response pathways. This 150 kDa protein is a member of the mitogen-activated protein kinase kinase kinase (MAP3K) family. ASK1 is expressed in several tissues including the heart brain liver and pancreas. Mechanically ASK1 gets activated in response to a variety of stress stimuli such as oxidative stress and endoplasmic reticulum stress which significantly impact the regulation of programmed cell death.
- Biological function summary
In response to stressors ASK1 activates the c-Jun N-terminal kinase (JNK) and p38 MAPK signaling pathways which play roles in apoptosis and inflammation. ASK1 tends to form a complex with TRAF2 and TRAF6 key adapters in downstream signaling. This kinase acts as an important link bridging environmental stress signals to cellular responses. Through its function ASK1 influences cell fate decisions in response to various stress conditions.
- Pathways
ASK1 is a central player in the MAPK signaling cascade. Within this cascade ASK1 regulates JNK and p38 MAPK pathways which are significant in stress adaptation and cellular responses. ASK1 interacts with proteins like MKK4 and MKK7 in the JNK pathway amplifying the stress signals. Through these interactions ASK1 ensures the propagation of signals important for adaptive responses or programmed cell death.
- Associated diseases and disorders
Dysfunction in ASK1 activity associates with neurodegenerative diseases and cardiovascular disorders. In Alzheimer's disease for instance ASK1 contributes to neuronal apoptosis via its engagement with JNK signaling. In cardiovascular disorders ASK1 exacerbates heart failure by promoting cell death and inflammation. ASK1's interaction with proteins such as TNF receptor-associated factor 2 (TRAF2) highlights its role in these pathological conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-ASK1 antibody - C-terminal (ab137339)
5% SDS PAGE
All lanes: Western blot - Anti-ASK1 antibody - C-terminal (ab137339) at 1/1000 dilution
All lanes: HeLa S3 whole cell lysate at 30 µg
Predicted band size: 155 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASK1 antibody - C-terminal (ab137339)
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft labelling ASK1 with ab137339 at 1/500 dilution.
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