Skip to main content

Rabbit Recombinant Monoclonal ASK1 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse samples. Cited in 38 publications.


Images

Western blot - Anti-ASK1 antibody [EP553Y] (AB45178), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-ASK1 antibody [EP553Y] (AB45178), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-ASK1 antibody [EP553Y] (AB45178), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASK1 antibody [EP553Y] (AB45178), expandable thumbnail
  • Western blot - Anti-ASK1 antibody [EP553Y] (AB45178), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Expected
Tested
Mouse
Expected
Expected
Tested
Expected

Tested
Tested

Species

Human

Dilution info

1/50

Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

For unpurified use at 1/2000

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/100 - 1/250

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100

Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Mediates signaling for determination of cell fate such as differentiation and survival. Plays a crucial role in the apoptosis signal transduction pathway through mitochondria-dependent caspase activation. MAP3K5/ASK1 is required for the innate immune response, which is essential for host defense against a wide range of pathogens. Mediates signal transduction of various stressors like oxidative stress as well as by receptor-mediated inflammatory signals, such as the tumor necrosis factor (TNF) or lipopolysaccharide (LPS). Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade and the p38 MAPK signal transduction cascade through the phosphorylation and activation of several MAP kinase kinases like MAP2K4/SEK1, MAP2K3/MKK3, MAP2K6/MKK6 and MAP2K7/MKK7. These MAP2Ks in turn activate p38 MAPKs and c-jun N-terminal kinases (JNKs). Both p38 MAPK and JNKs control the transcription factors activator protein-1 (AP-1).

Alternative names

Mitogen-activated protein kinase kinase kinase 5, Apoptosis signal-regulating kinase 1, MAPK/ERK kinase kinase 5, ASK-1, MEK kinase 5, MEKK 5, MAP3K5, ASK1, MAPKKK5, MEKK5

Recommended products

Rabbit Recombinant Monoclonal ASK1 antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse samples. Cited in 38 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EP553Y

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The ASK1 protein also known as apoptosis signal-regulating kinase 1 is an important component in cellular stress response pathways. This 150 kDa protein is a member of the mitogen-activated protein kinase kinase kinase (MAP3K) family. ASK1 is expressed in several tissues including the heart brain liver and pancreas. Mechanically ASK1 gets activated in response to a variety of stress stimuli such as oxidative stress and endoplasmic reticulum stress which significantly impact the regulation of programmed cell death.

Biological function summary

In response to stressors ASK1 activates the c-Jun N-terminal kinase (JNK) and p38 MAPK signaling pathways which play roles in apoptosis and inflammation. ASK1 tends to form a complex with TRAF2 and TRAF6 key adapters in downstream signaling. This kinase acts as an important link bridging environmental stress signals to cellular responses. Through its function ASK1 influences cell fate decisions in response to various stress conditions.

Pathways

ASK1 is a central player in the MAPK signaling cascade. Within this cascade ASK1 regulates JNK and p38 MAPK pathways which are significant in stress adaptation and cellular responses. ASK1 interacts with proteins like MKK4 and MKK7 in the JNK pathway amplifying the stress signals. Through these interactions ASK1 ensures the propagation of signals important for adaptive responses or programmed cell death.

Associated diseases and disorders

Dysfunction in ASK1 activity associates with neurodegenerative diseases and cardiovascular disorders. In Alzheimer's disease for instance ASK1 contributes to neuronal apoptosis via its engagement with JNK signaling. In cardiovascular disorders ASK1 exacerbates heart failure by promoting cell death and inflammation. ASK1's interaction with proteins such as TNF receptor-associated factor 2 (TRAF2) highlights its role in these pathological conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-ASK1 antibody [EP553Y] (ab45178), expandable thumbnail

    Western blot - Anti-ASK1 antibody [EP553Y] (ab45178)

    Lane 1: Wild-type HAP1 cell lysate (20 μg)
    Lane 2: ASK1 knockout HAP1 cell lysate (20 μg)
    Lane 3: HeLa cell lysate (20 μg)
    Lane 4: A549 cell lysate (20 μg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab45178 observed at 155, 160 kDa. Red - loading control, Agarose Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody ab1240, observed at 124 kDa.

    Unpurified ab45178 was shown to recognize ASK1 when ASK1 knockout samples were used, along with additional cross-reactive bands. Wild-type and ASK1 knockout samples were subjected to SDS-PAGE. ab45178 and Agarose Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody ab1240 (loading control to Vinculin) were diluted to 1/2000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-ASK1 antibody [EP553Y] (ab45178)

    Predicted band size: 155 kDa

  • Flow Cytometry (Intracellular) - Anti-ASK1 antibody [EP553Y] (ab45178), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-ASK1 antibody [EP553Y] (ab45178)

    Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling ASK1 with purified ab45178 at 1/100 dilution (10 μg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Immunocytochemistry/ Immunofluorescence - Anti-ASK1 antibody [EP553Y] (ab45178), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-ASK1 antibody [EP553Y] (ab45178)

    Immunocytochemistry/ Immunofluorescence analysis of RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) cells labeling ASK1 with Purified ab45178 at 1:100 (9.9 μg/ml). Cells were fixed in 100% Methanol. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 μg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASK1 antibody [EP553Y] (ab45178), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASK1 antibody [EP553Y] (ab45178)

    Unpurified ab45178 (1/10) staining human ASK1 in human lung carcinoma by immunohistochemistry using paraffin embedded tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Western blot - Anti-ASK1 antibody [EP553Y] (ab45178), expandable thumbnail

    Western blot - Anti-ASK1 antibody [EP553Y] (ab45178)

    All lanes: Western blot - Anti-ASK1 antibody [EP553Y] (ab45178) at 1/2000 dilution

    All lanes: HeLa cell lysate at 10 µg

    Secondary

    All lanes: Goat anti-rabbit, HRP labelled at 1/2000 dilution

    Predicted band size: 155 kDa

    Observed band size: 155 kDa

  • Flow Cytometry (Intracellular) - Anti-ASK1 antibody [EP553Y] (ab45178), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-ASK1 antibody [EP553Y] (ab45178)

    Overlay histogram showing HeLa cells stained with unpurified ab45178 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab45178, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was a goat anti-rabbit DyLight® 488 (IgG H+L) (Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com