Rabbit Recombinant Monoclonal ASK1 antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse samples.
Images
![Flow Cytometry (Intracellular) - Anti-ASK1 antibody [EP553Y] - BSA and Azide free (AB177030), expandable thumbnail](https://content.abcam.com/products/images/ask1-antibody-ep553y-bsa-and-azide-free-ab177030--flow-cytometry-intracellular-img37728.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-ASK1 antibody [EP553Y] - BSA and Azide free (AB177030)")
![Immunocytochemistry/ Immunofluorescence - Anti-ASK1 antibody [EP553Y] - BSA and Azide free (AB177030), expandable thumbnail](https://content.abcam.com/products/images/ask1-antibody-ep553y-bsa-and-azide-free-ab177030--immunocytochemistry-immunofluorescence-img24596.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-ASK1 antibody [EP553Y] - BSA and Azide free (AB177030)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASK1 antibody [EP553Y] - BSA and Azide free (AB177030), expandable thumbnail](https://content.abcam.com/products/images/ask1-antibody-ep553y-bsa-and-azide-free-ab177030--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img139249.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASK1 antibody [EP553Y] - BSA and Azide free (AB177030)")
![Immunocytochemistry/ Immunofluorescence - Anti-ASK1 antibody [EP553Y] - BSA and Azide free (AB177030), expandable thumbnail](https://content.abcam.com/products/images/ask1-antibody-ep553y-bsa-and-azide-free-ab177030--immunocytochemistry-immunofluorescence-img70811.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-ASK1 antibody [EP553Y] - BSA and Azide free (AB177030)")
![Flow Cytometry (Intracellular) - Anti-ASK1 antibody [EP553Y] - BSA and Azide free (AB177030), expandable thumbnail](https://content.abcam.com/products/images/ask1-antibody-ep553y-bsa-and-azide-free-ab177030--flow-cytometry-intracellular-img80353.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-ASK1 antibody [EP553Y] - BSA and Azide free (AB177030)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Constituents: PBS
- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Expected | Expected | Tested |
| Mouse | Expected | Not recommended | Expected | Tested | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
8 products for Alternative Version
Target data
Function
Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Mediates signaling for determination of cell fate such as differentiation and survival. Plays a crucial role in the apoptosis signal transduction pathway through mitochondria-dependent caspase activation. MAP3K5/ASK1 is required for the innate immune response, which is essential for host defense against a wide range of pathogens. Mediates signal transduction of various stressors like oxidative stress as well as by receptor-mediated inflammatory signals, such as the tumor necrosis factor (TNF) or lipopolysaccharide (LPS). Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade and the p38 MAPK signal transduction cascade through the phosphorylation and activation of several MAP kinase kinases like MAP2K4/SEK1, MAP2K3/MKK3, MAP2K6/MKK6 and MAP2K7/MKK7. These MAP2Ks in turn activate p38 MAPKs and c-jun N-terminal kinases (JNKs). Both p38 MAPK and JNKs control the transcription factors activator protein-1 (AP-1).
Alternative names
Mitogen-activated protein kinase kinase kinase 5, Apoptosis signal-regulating kinase 1, MAPK/ERK kinase kinase 5, ASK-1, MEK kinase 5, MEKK 5, MAP3K5, ASK1, MAPKKK5, MEKK5
Recommended products
Rabbit Recombinant Monoclonal ASK1 antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse samples.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Constituents: PBS
- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EP553Y
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab177030 is the carrier-free version of Anti-ASK1 antibody [EP553Y] ab45178.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The ASK1 protein also known as apoptosis signal-regulating kinase 1 is an important component in cellular stress response pathways. This 150 kDa protein is a member of the mitogen-activated protein kinase kinase kinase (MAP3K) family. ASK1 is expressed in several tissues including the heart brain liver and pancreas. Mechanically ASK1 gets activated in response to a variety of stress stimuli such as oxidative stress and endoplasmic reticulum stress which significantly impact the regulation of programmed cell death.
- Biological function summary
In response to stressors ASK1 activates the c-Jun N-terminal kinase (JNK) and p38 MAPK signaling pathways which play roles in apoptosis and inflammation. ASK1 tends to form a complex with TRAF2 and TRAF6 key adapters in downstream signaling. This kinase acts as an important link bridging environmental stress signals to cellular responses. Through its function ASK1 influences cell fate decisions in response to various stress conditions.
- Pathways
ASK1 is a central player in the MAPK signaling cascade. Within this cascade ASK1 regulates JNK and p38 MAPK pathways which are significant in stress adaptation and cellular responses. ASK1 interacts with proteins like MKK4 and MKK7 in the JNK pathway amplifying the stress signals. Through these interactions ASK1 ensures the propagation of signals important for adaptive responses or programmed cell death.
- Associated diseases and disorders
Dysfunction in ASK1 activity associates with neurodegenerative diseases and cardiovascular disorders. In Alzheimer's disease for instance ASK1 contributes to neuronal apoptosis via its engagement with JNK signaling. In cardiovascular disorders ASK1 exacerbates heart failure by promoting cell death and inflammation. ASK1's interaction with proteins such as TNF receptor-associated factor 2 (TRAF2) highlights its role in these pathological conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Flow Cytometry (Intracellular) - Anti-ASK1 antibody [EP553Y] - BSA and Azide free (ab177030), expandable thumbnail](https://content.abcam.com/products/images/ask1-antibody-ep553y-bsa-and-azide-free-ab177030--flow-cytometry-intracellular-img37728.jpg "Flow Cytometry (Intracellular) - Anti-ASK1 antibody [EP553Y] - BSA and Azide free (ab177030)")
Flow Cytometry (Intracellular) - Anti-ASK1 antibody [EP553Y] - BSA and Azide free (ab177030)
Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling ASK1 with purified Anti-ASK1 antibody [EP553Y] ab45178 at 1/100 dilution (10 μg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue). This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ASK1 antibody [EP553Y] ab45178).
![Immunocytochemistry/ Immunofluorescence - Anti-ASK1 antibody [EP553Y] - BSA and Azide free (ab177030), expandable thumbnail](https://content.abcam.com/products/images/ask1-antibody-ep553y-bsa-and-azide-free-ab177030--immunocytochemistry-immunofluorescence-img24596.jpg "Immunocytochemistry/ Immunofluorescence - Anti-ASK1 antibody [EP553Y] - BSA and Azide free (ab177030)")
Immunocytochemistry/ Immunofluorescence - Anti-ASK1 antibody [EP553Y] - BSA and Azide free (ab177030)
Immunocytochemistry/ Immunofluorescence analysis of RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) cells labeling ASK1 with Purified Anti-ASK1 antibody [EP553Y] ab45178 at 1:100 (9.9 µg/ml). Cells were fixed in 100% Methanol. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 ?g/ml). Goat anti rabbit IgG (Alexa Fluor? 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 ?g/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ASK1 antibody [EP553Y] ab45178).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASK1 antibody [EP553Y] - BSA and Azide free (ab177030), expandable thumbnail](https://content.abcam.com/products/images/ask1-antibody-ep553y-bsa-and-azide-free-ab177030--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img139249.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASK1 antibody [EP553Y] - BSA and Azide free (ab177030)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASK1 antibody [EP553Y] - BSA and Azide free (ab177030)
Unpurified Anti-ASK1 antibody [EP553Y] ab45178 (1/10) staining human ASK1 in human lung carcinoma by immunohistochemistry using paraffin embedded tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ASK1 antibody [EP553Y] ab45178).
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunocytochemistry/ Immunofluorescence - Anti-ASK1 antibody [EP553Y] - BSA and Azide free (ab177030), expandable thumbnail](https://content.abcam.com/products/images/ask1-antibody-ep553y-bsa-and-azide-free-ab177030--immunocytochemistry-immunofluorescence-img70811.jpg "Immunocytochemistry/ Immunofluorescence - Anti-ASK1 antibody [EP553Y] - BSA and Azide free (ab177030)")
Immunocytochemistry/ Immunofluorescence - Anti-ASK1 antibody [EP553Y] - BSA and Azide free (ab177030)
Immunofluorescent staining of HeLa cells using unpurified Anti-ASK1 antibody [EP553Y] ab45178 (1/10).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ASK1 antibody [EP553Y] ab45178).
![Flow Cytometry (Intracellular) - Anti-ASK1 antibody [EP553Y] - BSA and Azide free (ab177030), expandable thumbnail](https://content.abcam.com/products/images/ask1-antibody-ep553y-bsa-and-azide-free-ab177030--flow-cytometry-intracellular-img80353.jpg "Flow Cytometry (Intracellular) - Anti-ASK1 antibody [EP553Y] - BSA and Azide free (ab177030)")
Flow Cytometry (Intracellular) - Anti-ASK1 antibody [EP553Y] - BSA and Azide free (ab177030)
Overlay histogram showing HeLa cells stained with unpurified Anti-ASK1 antibody [EP553Y] ab45178 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (Anti-ASK1 antibody [EP553Y] ab45178, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was a goat anti-rabbit DyLight® 488 (IgG H+L) (Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ASK1 antibody [EP553Y] ab45178).
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