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AB278547

Anti-ASK1 (phospho S83) antibody [Ask1S83-G4]

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(9 Publications)

Rabbit Recombinant Monoclonal ASK1 phospho S83 antibody. Suitable for Flow Cyt, WB and reacts with Mouse, Rat, Human samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human MAP3K5 phospho S83.

View Alternative Names

ASK1, MAPKKK5, MEKK5, MAP3K5, Mitogen-activated protein kinase kinase kinase 5, Apoptosis signal-regulating kinase 1, MAPK/ERK kinase kinase 5, ASK-1, MEK kinase 5, MEKK 5

5 Images
Flow Cytometry - Anti-ASK1 (phospho S83) antibody [Ask1S83-G4] (AB278547)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-ASK1 (phospho S83) antibody [Ask1S83-G4] (AB278547)

Flow cytometric analysis of K562 cells secondary antibody only negative control (blue) or treated with imatinib (red) or with IFNa + IL-4 + pervanadate (green) using ab278547 at 5ng/mL.

Flow Cytometry - Anti-ASK1 (phospho S83) antibody [Ask1S83-G4] (AB278547)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-ASK1 (phospho S83) antibody [Ask1S83-G4] (AB278547)

Peptide blocking flow cytometric analysis of C6 cells secondary antibody only negative control (light blue) or imatinib-treated (red) or pervandadate-treated (green) or imatinib and blocked with phospho-peptide (black) or pervanadate and blocked with phospho peptide (gold) or imatinib and blocked with non-phospho peptide (dark blue) or pervanadate and blocked with non-phospho peptide (purple) using ab278547 at 1ng/mL.

Flow Cytometry - Anti-ASK1 (phospho S83) antibody [Ask1S83-G4] (AB278547)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-ASK1 (phospho S83) antibody [Ask1S83-G4] (AB278547)

Flow cytometric analysis of C6 cells secondary antibody only negative control (blue) or treated with imatinib (red) or with pervanadate (green) using ab278547 at 1ng/mL.

Western blot - Anti-ASK1 (phospho S83) antibody [Ask1S83-G4] (AB278547)
  • WB

Supplier Data

Western blot - Anti-ASK1 (phospho S83) antibody [Ask1S83-G4] (AB278547)

All lanes:

Western blot - Anti-ASK1 (phospho S83) antibody [Ask1S83-G4] (ab278547) at 0.01 µg/mL

Lane 1:

C6 cell extract treated with imatinib

Lane 2:

C6 cell extract treated with PV

Predicted band size: 155 kDa

false

Western blot - Anti-ASK1 (phospho S83) antibody [Ask1S83-G4] (AB278547)
  • WB

Supplier Data

Western blot - Anti-ASK1 (phospho S83) antibody [Ask1S83-G4] (AB278547)

All lanes:

Western blot - Anti-ASK1 (phospho S83) antibody [Ask1S83-G4] (ab278547) at 0.01 µg/mL

All lanes:

L929 cell extract treated with 20% FBS

Predicted band size: 155 kDa

false

  • 519 FITC

    FITC Anti-ASK1 (phospho S83) antibody [Ask1S83-G4]

  • 660 APC

    APC Anti-ASK1 (phospho S83) antibody [Ask1S83-G4]

  • 578 PE

    PE Anti-ASK1 (phospho S83) antibody [Ask1S83-G4]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

Ask1S83-G4

Isotype

IgG

Light chain type

kappa

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

Flow Cyt, WB

applications

Immunogen

Synthetic Peptide within Human MAP3K5 phospho S83. The exact immunogen used to generate this antibody is proprietary information.

Q99683

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.01 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.001-1 µg/mL", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.001-1 µg/mL", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.01 µg/mL", "WB-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ASK1 protein also known as apoptosis signal-regulating kinase 1 is an important component in cellular stress response pathways. This 150 kDa protein is a member of the mitogen-activated protein kinase kinase kinase (MAP3K) family. ASK1 is expressed in several tissues including the heart brain liver and pancreas. Mechanically ASK1 gets activated in response to a variety of stress stimuli such as oxidative stress and endoplasmic reticulum stress which significantly impact the regulation of programmed cell death.
Biological function summary

In response to stressors ASK1 activates the c-Jun N-terminal kinase (JNK) and p38 MAPK signaling pathways which play roles in apoptosis and inflammation. ASK1 tends to form a complex with TRAF2 and TRAF6 key adapters in downstream signaling. This kinase acts as an important link bridging environmental stress signals to cellular responses. Through its function ASK1 influences cell fate decisions in response to various stress conditions.

Pathways

ASK1 is a central player in the MAPK signaling cascade. Within this cascade ASK1 regulates JNK and p38 MAPK pathways which are significant in stress adaptation and cellular responses. ASK1 interacts with proteins like MKK4 and MKK7 in the JNK pathway amplifying the stress signals. Through these interactions ASK1 ensures the propagation of signals important for adaptive responses or programmed cell death.

Dysfunction in ASK1 activity associates with neurodegenerative diseases and cardiovascular disorders. In Alzheimer's disease for instance ASK1 contributes to neuronal apoptosis via its engagement with JNK signaling. In cardiovascular disorders ASK1 exacerbates heart failure by promoting cell death and inflammation. ASK1's interaction with proteins such as TNF receptor-associated factor 2 (TRAF2) highlights its role in these pathological conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Mediates signaling for determination of cell fate such as differentiation and survival. Plays a crucial role in the apoptosis signal transduction pathway through mitochondria-dependent caspase activation. MAP3K5/ASK1 is required for the innate immune response, which is essential for host defense against a wide range of pathogens. Mediates signal transduction of various stressors like oxidative stress as well as by receptor-mediated inflammatory signals, such as the tumor necrosis factor (TNF) or lipopolysaccharide (LPS). Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade and the p38 MAPK signal transduction cascade through the phosphorylation and activation of several MAP kinase kinases like MAP2K4/SEK1, MAP2K3/MKK3, MAP2K6/MKK6 and MAP2K7/MKK7. These MAP2Ks in turn activate p38 MAPKs and c-jun N-terminal kinases (JNKs). Both p38 MAPK and JNKs control the transcription factors activator protein-1 (AP-1).
See full target information MAP3K5 phospho S83
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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Physiological reports 13:e70553 PubMed40969143

2025

Midkine promotes PDGF-BB-induced proliferation, migration, and glycolysis of airway smooth muscle cells via the PI3K Akt pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yongtian Xu,Wenli Li,Jun Shi,Yanfang Guo

Redox report : communications in free radical research 29:2416825 PubMed39410845

2024

Selenium participates in the formation of kidney stones by alleviating endoplasmic reticulum stress and apoptosis of renal tubular epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaozhe Su,Hongbo Chen,Heng Xiang,Hu Ke,Caitao Dong,Qianlin Song,Jiawei Zhou,Qinhong Jiang,Yunhan Wang,Liang Chen,Sixing Yang

The Journal of biological chemistry 300:107394 PubMed38768813

2024

Kaempferol attenuates particle-induced osteogenic impairment by regulating ER stress via the IRE1α-XBP1s pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Yu,Zhengrong Ren,Yuxiang Wang,Guodong Yuan,Jianlun Hu,Lin Song,Cheng Pan,Kangkang Feng,Yuqiao Liu,Longgang Shao,Li Zhang,Jinjuan Wang,Jianning Zhao,Nirong Bao,Zhongyang Sun

Journal of neuroinflammation 20:222 PubMed37777772

2023

Inhibition of neutrophil extracellular trap formation ameliorates neuroinflammation and neuronal apoptosis via STING-dependent IRE1α/ASK1/JNK signaling pathway in mice with traumatic brain injury.

Applications

Unspecified application

Species

Unspecified reactive species

Guihong Shi,Liang Liu,Yiyao Cao,Guangshuo Ma,Yanlin Zhu,Jianye Xu,Xu Zhang,Tuo Li,Liang Mi,Haoran Jia,Yanfeng Zhang,Xilei Liu,Yuan Zhou,Shenghui Li,Guili Yang,Xiao Liu,Fanglian Chen,Baolong Wang,Quanjun Deng,Shu Zhang,Jianning Zhang

Molecules (Basel, Switzerland) 28: PubMed37764275

2023

Soybean Antigen Protein-Induced Intestinal Barrier Damage by Trigging Endoplasmic Reticulum Stress and Disordering Gut Microbiota in Weaned Piglets.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Wang,Yujia Liu,Daoliang Zhang,Hongyan Ding,Shibin Feng,Chang Zhao,Jinjie Wu,Xichun Wang

Experimental and therapeutic medicine 26:450 PubMed37614418

2023

FOXP1‑induced DUSP12 alleviates vascular endothelial cell inflammation and oxidative stress injury induced by ox‑LDL via MAP3K5 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanmin Li,Long Gu,Jian Zhou,Chenjun Han,Wangfu Zang

PloS one 18:e0286903 PubMed37310976

2023

The angiotensin receptor neprilysin inhibitor LCZ696 attenuates renal fibrosis via ASK1/JNK/p38 MAPK-mediated apoptosis in unilateral ureteral obstruction.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Ding,Sheng Cui,Song Yu Li,Lin Yan Cui,Qi Yan Nan,Xue Jing Lin,Mei Ying Xuan,Jian Jin,Shang Guo Piao,Yu Ji Jiang,Hai Lan Zheng,Ji Zhe Jin,Byung Ha Chung,Chul Woo Yang,Jing Hao Cui,Can Li

PeerJ 11:e15315 PubMed37220525

2023

Hyperoside alleviates doxorubicin-induced myocardial cells apoptosis by inhibiting the apoptosis signal-regulating kinase 1/p38 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Lingxia Chen,Zhi Qin,Zhong-Bao Ruan

Cell cycle (Georgetown, Tex.) 20:1907-1922 PubMed34427546

2021

Ketamine enhances autophagy and endoplasmic reticulum stress in rats and SV-HUC-1 cells via activating IRE1-TRAF2-ASK1-JNK pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yanming Yu,Daoxu Wu,Yongwei Li,Hui Qiao,Zhengfei Shan
View all publications
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Product promise

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