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Rabbit Recombinant Monoclonal ASK1 phospho T838 antibody. Suitable for Flow Cyt and reacts with Mouse, Human samples. Immunogen corresponding to Synthetic Peptide within Human MAP3K5 phospho T838.

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Images

Flow Cytometry - Anti-ASK1 (phospho T838) antibody [Ask1T838-8D12] (AB278644), expandable thumbnail
  • Flow Cytometry - Anti-ASK1 (phospho T838) antibody [Ask1T838-8D12] (AB278644), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49.88% PBS, 0.1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Synthetic Peptide within Human MAP3K5 phospho T838. The exact immunogen used to generate this antibody is proprietary information. Database link Q99683

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt
Human
Expected
Mouse
Tested

Tested
Tested

Species
Mouse
Dilution info
0.001-1 µg/mL
Notes

-

Expected
Expected

Species
Human
Dilution info
0.001-1 µg/mL
Notes

-

Target data

Function

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Mediates signaling for determination of cell fate such as differentiation and survival. Plays a crucial role in the apoptosis signal transduction pathway through mitochondria-dependent caspase activation. MAP3K5/ASK1 is required for the innate immune response, which is essential for host defense against a wide range of pathogens. Mediates signal transduction of various stressors like oxidative stress as well as by receptor-mediated inflammatory signals, such as the tumor necrosis factor (TNF) or lipopolysaccharide (LPS). Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade and the p38 MAPK signal transduction cascade through the phosphorylation and activation of several MAP kinase kinases like MAP2K4/SEK1, MAP2K3/MKK3, MAP2K6/MKK6 and MAP2K7/MKK7. These MAP2Ks in turn activate p38 MAPKs and c-jun N-terminal kinases (JNKs). Both p38 MAPK and JNKs control the transcription factors activator protein-1 (AP-1).

Alternative names

ASK1, MAPKKK5, MEKK5, MAP3K5, Mitogen-activated protein kinase kinase kinase 5, Apoptosis signal-regulating kinase 1, MAPK/ERK kinase kinase 5, ASK-1, MEK kinase 5, MEKK 5

Recommended products

Rabbit Recombinant Monoclonal ASK1 phospho T838 antibody. Suitable for Flow Cyt and reacts with Mouse, Human samples. Immunogen corresponding to Synthetic Peptide within Human MAP3K5 phospho T838.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Synthetic Peptide within Human MAP3K5 phospho T838. The exact immunogen used to generate this antibody is proprietary information. Database link Q99683
Clone number
Ask1T838-8D12
Purification technique
Affinity purification Protein A/G
Light chain type
kappa
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The ASK1 protein also known as apoptosis signal-regulating kinase 1 is an important component in cellular stress response pathways. This 150 kDa protein is a member of the mitogen-activated protein kinase kinase kinase (MAP3K) family. ASK1 is expressed in several tissues including the heart brain liver and pancreas. Mechanically ASK1 gets activated in response to a variety of stress stimuli such as oxidative stress and endoplasmic reticulum stress which significantly impact the regulation of programmed cell death.

Biological function summary

In response to stressors ASK1 activates the c-Jun N-terminal kinase (JNK) and p38 MAPK signaling pathways which play roles in apoptosis and inflammation. ASK1 tends to form a complex with TRAF2 and TRAF6 key adapters in downstream signaling. This kinase acts as an important link bridging environmental stress signals to cellular responses. Through its function ASK1 influences cell fate decisions in response to various stress conditions.

Pathways

ASK1 is a central player in the MAPK signaling cascade. Within this cascade ASK1 regulates JNK and p38 MAPK pathways which are significant in stress adaptation and cellular responses. ASK1 interacts with proteins like MKK4 and MKK7 in the JNK pathway amplifying the stress signals. Through these interactions ASK1 ensures the propagation of signals important for adaptive responses or programmed cell death.

Associated diseases and disorders

Dysfunction in ASK1 activity associates with neurodegenerative diseases and cardiovascular disorders. In Alzheimer's disease for instance ASK1 contributes to neuronal apoptosis via its engagement with JNK signaling. In cardiovascular disorders ASK1 exacerbates heart failure by promoting cell death and inflammation. ASK1's interaction with proteins such as TNF receptor-associated factor 2 (TRAF2) highlights its role in these pathological conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Flow Cytometry - Anti-ASK1 (phospho T838) antibody [Ask1T838-8D12] (ab278644), expandable thumbnail

    Flow Cytometry - Anti-ASK1 (phospho T838) antibody [Ask1T838-8D12] (ab278644)

    Flow cytometric analysis of NIH3T3 cells treated with staurosporine and unstained as negative control (blue) or treated with staurosporine (red) or with PDGF (green) and stained using ab278644 at 5ng/mL.

  • Flow Cytometry - Anti-ASK1 (phospho T838) antibody [Ask1T838-8D12] (ab278644), expandable thumbnail

    Flow Cytometry - Anti-ASK1 (phospho T838) antibody [Ask1T838-8D12] (ab278644)

    Peptide blocking flow cytometric analysis of 3T3 cells secondary antibody only negative control (light blue) or staurosporine-treated (red) or PDGF-treated (green) or staurosporine and blocked with phospho-peptide (black) or PDGF and blocked with phospho peptide (gold) or staurosporine and blocked with non-phospho peptide (dark blue) or PDGF and blocked with non-phospho peptide (purple) using ab278644 at 10ng/mL.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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