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AB227919

Anti-ASMT antibody - C-terminal

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(1 Publication)

Rabbit Polyclonal ASMT antibody. C-terminal. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ASMT.

View Alternative Names

Acetylserotonin O-methyltransferase, Hydroxyindole O-methyltransferase, HIOMT, ASMT

2 Images
Western blot - Anti-ASMT antibody - C-terminal (AB227919)
  • WB

Supplier Data

Western blot - Anti-ASMT antibody - C-terminal (AB227919)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-ASMT antibody - C-terminal (ab227919) at 1/1000 dilution

All lanes:

ARPE-19 Whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-ASMT antibody - C-terminal (AB227919)
  • WB

Supplier Data

Western blot - Anti-ASMT antibody - C-terminal (AB227919)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-ASMT antibody - C-terminal (ab227919) at 1/1000 dilution

Lane 1:

Mouse brain tissue extracts at 50 µg

Lane 2:

Rat brain tissue extracts at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse, Rat

Applications

WB

applications

Immunogen

Synthetic Peptide within Human ASMT. The exact immunogen used to generate this antibody is proprietary information.

P46597

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Sheep": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ASMT also known as acetylserotonin O-methyltransferase or hydroxyindole O-methyltransferase is an enzyme with a molecular weight of approximately 41-45 kDa. It facilitates the transfer of a methyl group from S-adenosylmethionine to N-acetylserotonin producing melatonin. ASMT is widely expressed in the pineal gland but its presence is also noted in other tissues including the retina and gastrointestinal tract. Its activity plays a critical role in synthesizing melatonin a hormone associated with regulating circadian rhythms.
Biological function summary

ASMT contributes to the synthesis of melatonin. It acts as the last enzyme in the melatonin biosynthesis pathway. Melatonin not only regulates the sleep-wake cycle but also influences various physiological processes. While ASMT is not part of a large protein complex its interaction with substrates such as N-acetylserotonin and S-adenosylmethionine highlights its functional relationship within the biosynthetic machinery.

Pathways

ASMT plays a significant role in the serotonin-melatonin pathway. This pathway starts with tryptophan which converts to serotonin and culminates in melatonin synthesis. ASMT collaborates with other enzymes such as tryptophan hydroxylase and serotonin N-acetyltransferase in these pathways. It directly influences circadian rhythm regulation and light-dark cycle adaptation through melatonin production.

ASMT shows a connection to sleep disorders and certain mood disorders like depression. Disruption in melatonin levels often implicates abnormalities in ASMT function. This disruption also associates ASMT with altered serotonin levels implicating its relationship with serotonin receptor-based pathways present in mood disorders. Researchers continue to explore ASMT’s role looking for therapeutic advancements.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Isoform 1. Catalyzes the transfer of a methyl group onto N-acetylserotonin, producing melatonin (N-acetyl-5-methoxytryptamine).. Isoform 2. Does not show Acetylserotonin O-methyltransferase activity.. Isoform 3. Does not show Acetylserotonin O-methyltransferase activity.
See full target information ASMT
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 37:e23085 PubMed37462502

2023

DNMT3B activates FGFR3-mediated endoplasmic reticulum stress by regulating PTPN2 promoter methylation to promote the development of atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiwen Zhang,Quan Guo,Zhenzhou Zhao,Ming Nie,Qingbo Shi,En Li,Kaiyuan Liu,Haosen Yu,Lixin Rao,Muwei Li
View all publications
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Product promise

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