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AB180511

Anti-ASMT antibody [EPR12458]

5

(1 Review)

|

(7 Publications)

Rabbit Recombinant Monoclonal ASMT antibody. Suitable for WB and reacts with Human samples. Cited in 7 publications.

View Alternative Names

Acetylserotonin O-methyltransferase, Hydroxyindole O-methyltransferase, HIOMT, ASMT

1 Images
Western blot - Anti-ASMT antibody [EPR12458] (AB180511)
  • WB

Supplier Data

Western blot - Anti-ASMT antibody [EPR12458] (AB180511)

5% NFDM/TBST used as blocking and dilution buffer.

All lanes:

Western blot - Anti-ASMT antibody [EPR12458] (ab180511) at 1/1000 dilution

All lanes:

Y79 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 38 kDa

false

  • Carrier free

    Anti-ASMT antibody [EPR12458] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12458

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ASMT also known as acetylserotonin O-methyltransferase or hydroxyindole O-methyltransferase is an enzyme with a molecular weight of approximately 41-45 kDa. It facilitates the transfer of a methyl group from S-adenosylmethionine to N-acetylserotonin producing melatonin. ASMT is widely expressed in the pineal gland but its presence is also noted in other tissues including the retina and gastrointestinal tract. Its activity plays a critical role in synthesizing melatonin a hormone associated with regulating circadian rhythms.
Biological function summary

ASMT contributes to the synthesis of melatonin. It acts as the last enzyme in the melatonin biosynthesis pathway. Melatonin not only regulates the sleep-wake cycle but also influences various physiological processes. While ASMT is not part of a large protein complex its interaction with substrates such as N-acetylserotonin and S-adenosylmethionine highlights its functional relationship within the biosynthetic machinery.

Pathways

ASMT plays a significant role in the serotonin-melatonin pathway. This pathway starts with tryptophan which converts to serotonin and culminates in melatonin synthesis. ASMT collaborates with other enzymes such as tryptophan hydroxylase and serotonin N-acetyltransferase in these pathways. It directly influences circadian rhythm regulation and light-dark cycle adaptation through melatonin production.

ASMT shows a connection to sleep disorders and certain mood disorders like depression. Disruption in melatonin levels often implicates abnormalities in ASMT function. This disruption also associates ASMT with altered serotonin levels implicating its relationship with serotonin receptor-based pathways present in mood disorders. Researchers continue to explore ASMT’s role looking for therapeutic advancements.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Isoform 1. Catalyzes the transfer of a methyl group onto N-acetylserotonin, producing melatonin (N-acetyl-5-methoxytryptamine).. Isoform 2. Does not show Acetylserotonin O-methyltransferase activity.. Isoform 3. Does not show Acetylserotonin O-methyltransferase activity.
See full target information ASMT
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Journal of biomedical science 32:78 PubMed40836335

2025

Smooth muscle-specific expression of hydroxyindole O-methyltransferase reduces arterial injury-induced intimal hyperplasia.

Applications

Unspecified application

Species

Unspecified reactive species

Wei-Cheng Jiang,Chung-Huang Chen,Hua-Hui Ho,Pei-Yu Gung,Jing-Yiing Wu,Cheng-Chin Kuo,Kenneth K Wu,Shaw-Fang Yet

Cellular and molecular life sciences : CMLS 82:104 PubMed40047877

2025

Melatonin refines ovarian mitochondrial dysfunction in PCOS by regulating the circadian rhythm gene Clock.

Applications

Unspecified application

Species

Unspecified reactive species

Wenxiu Chen,Hongyan Zhang,Bao Guo,Yumei Tao,Junhui Zhang,Jiayi Wang,Guangyi Chen,Mengting Cheng,Qiang Hong,Yunxia Cao,Fenfen Xie

Journal of pineal research 75:e12885 PubMed37183291

2023

RP58 knockdown contributes to hypoxia-ischemia-induced pineal dysfunction and circadian rhythm disruption in neonatal rats.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Yang,Zubin Zhang,Xin Ding,Xiaolu Jiang,Lanlan Tan,Chenjie Lin,Lixiao Xu,Gen Li,Lianghua Lu,Zhenghong Qin,Xing Feng,Mei Li

PloS one 15:e0241561 PubMed33253255

2020

The effect of autophagy and mitochondrial fission on Harderian gland is greater than apoptosis in male hamsters during different photoperiods.

Applications

Unspecified application

Species

Unspecified reactive species

Jin-Hui Xu,Zhe Wang,Jun-Jie Mou,Xiang-Yu Zhao,Xiao-Cui Geng,Ming Wu,Hui-Liang Xue,Lei Chen,Lai-Xiang Xu

Frontiers in physiology 11:408 PubMed32435203

2020

Photoperiod Affects Harderian Gland Morphology and Secretion in Female : Autophagy, Apoptosis, and Mitochondria.

Applications

Unspecified application

Species

Unspecified reactive species

Zhe Wang,Jin-Hui Xu,Jun-Jie Mou,Xiao-Tong Kong,Ming Wu,Hui-Liang Xue,Lai-Xiang Xu

Journal of pineal research 67:e12594 PubMed31286565

2019

Melatonin is a potential drug for the prevention of bone loss during space flight.

Applications

Unspecified application

Species

Unspecified reactive species

Mika Ikegame,Atsuhiko Hattori,Makoto J Tabata,Kei-Ichiro Kitamura,Yoshiaki Tabuchi,Yukihiro Furusawa,Yusuke Maruyama,Tatsuki Yamamoto,Toshio Sekiguchi,Risa Matsuoka,Taizo Hanmoto,Takahiro Ikari,Masato Endo,Katsunori Omori,Masaki Nakano,Sayaka Yashima,Sadakazu Ejiri,Toshiki Taya,Hiroshi Nakashima,Nobuaki Shimizu,Masahisa Nakamura,Takashi Kondo,Kazuichi Hayakawa,Ichiro Takasaki,Atsushi Kaminishi,Ryosuke Akatsuka,Yuichi Sasayama,Takumi Nishiuchi,Masayuki Nara,Hachiro Iseki,Vishwajit S Chowdhury,Shigehito Wada,Kenichi Ijiri,Toshio Takeuchi,Tohru Suzuki,Hironori Ando,Kouhei Matsuda,Masanori Somei,Hiroyuki Mishima,Yuko Mikuni-Takagaki,Hisayuki Funahashi,Akihisa Takahashi,Yoshinari Watanabe,Masahiro Maeda,Hideaki Uchida,Akio Hayashi,Akira Kambegawa,Azusa Seki,Sachiko Yano,Toru Shimazu,Hiromi Suzuki,Jun Hirayama,Nobuo Suzuki

The Journal of biological chemistry 293:11131-11142 PubMed29794137

2018

Restoration of hydroxyindole -methyltransferase levels in human cancer cells induces a tryptophan-metabolic switch and attenuates cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Hua-Ling Chen,Chao-Yun Yuan,Huei-Hsuan Cheng,Tzu-Ching Chang,Shau-Ku Huang,Cheng-Chin Kuo,Kenneth K Wu
View all publications
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Product promise

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