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AB236125

Anti-ASMT antibody [EPR12458] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal ASMT antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Acetylserotonin O-methyltransferase, Hydroxyindole O-methyltransferase, HIOMT, ASMT

1 Images
Western blot - Anti-ASMT antibody [EPR12458] - BSA and Azide free (AB236125)
  • WB

Supplier Data

Western blot - Anti-ASMT antibody [EPR12458] - BSA and Azide free (AB236125)

5% NFDM/TBST used as blocking and dilution buffer.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180511).

All lanes:

Western blot - Anti-ASMT antibody [EPR12458] (<a href='/en-us/products/primary-antibodies/asmt-antibody-epr12458-ab180511'>ab180511</a>) at 1/1000 dilution

All lanes:

Y79 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 38 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12458

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab236125 is the carrier-free version of ab180511.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ASMT also known as acetylserotonin O-methyltransferase or hydroxyindole O-methyltransferase is an enzyme with a molecular weight of approximately 41-45 kDa. It facilitates the transfer of a methyl group from S-adenosylmethionine to N-acetylserotonin producing melatonin. ASMT is widely expressed in the pineal gland but its presence is also noted in other tissues including the retina and gastrointestinal tract. Its activity plays a critical role in synthesizing melatonin a hormone associated with regulating circadian rhythms.
Biological function summary

ASMT contributes to the synthesis of melatonin. It acts as the last enzyme in the melatonin biosynthesis pathway. Melatonin not only regulates the sleep-wake cycle but also influences various physiological processes. While ASMT is not part of a large protein complex its interaction with substrates such as N-acetylserotonin and S-adenosylmethionine highlights its functional relationship within the biosynthetic machinery.

Pathways

ASMT plays a significant role in the serotonin-melatonin pathway. This pathway starts with tryptophan which converts to serotonin and culminates in melatonin synthesis. ASMT collaborates with other enzymes such as tryptophan hydroxylase and serotonin N-acetyltransferase in these pathways. It directly influences circadian rhythm regulation and light-dark cycle adaptation through melatonin production.

ASMT shows a connection to sleep disorders and certain mood disorders like depression. Disruption in melatonin levels often implicates abnormalities in ASMT function. This disruption also associates ASMT with altered serotonin levels implicating its relationship with serotonin receptor-based pathways present in mood disorders. Researchers continue to explore ASMT’s role looking for therapeutic advancements.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Isoform 1. Catalyzes the transfer of a methyl group onto N-acetylserotonin, producing melatonin (N-acetyl-5-methoxytryptamine).. Isoform 2. Does not show Acetylserotonin O-methyltransferase activity.. Isoform 3. Does not show Acetylserotonin O-methyltransferase activity.
See full target information ASMT
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of neuroinflammation 17:252 PubMed32859212

2020

Transgenic inhibition of astroglial NF-κB restrains the neuroinflammatory and neurodegenerative outcomes of experimental mouse glaucoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangjun Yang,Qun Zeng,Mine Barış,Gülgün Tezel
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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