Anti-ASPA antibody

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ASPA antibody (AB154503)

Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells labeling ASPA using ab154503 antibody at 1/500 dilution. Lower panel shows costaining with Hoechst 33342.

• WB

Unknown

Western blot - Anti-ASPA antibody (AB154503)

10% SDS PAGE

All lanes:

Western blot - Anti-ASPA antibody (ab154503) at 1/10000 dilution

All lanes:

Mouse brain whole cell lysate at 20 µg/mL

Predicted band size: 36 kDa

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• WB

Supplier Data

Western blot - Anti-ASPA antibody (AB154503)

Samples were separated by 10% SDS PAGE.

All lanes:

Western blot - Anti-ASPA antibody (ab154503) at 1/10000 dilution

Lane 1:

Non-transfected (–) 293T whole cell extracts at 30 µg

Lane 2:

ASPA transfected (+) 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 36 kDa

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• WB

CiteAb

Western blot - Anti-ASPA antibody (AB154503)

ASPA western blot using anti-ASPA antibody ab154503. Publication image and figure legend from Carpinelli, M. R., Voss, A. K., et al., 2014, Dis Model Mech, PubMed 24682784.

ab154503 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab154503 please see the product overview.

deaf14 mice harbor a nonsense mutation in Aspa. (A) Manhattan plot showing probability of linkage to deaf14 locus across the genome. A LOD score above 3 is indicative of linkage. This was observed for a single peak on chromosome 11, indicating that the deaf14 mutation is on this chromosome. (B) Haplotypes of 140 N1F1 mice for SNPs on chromosome 11. Mice were deemed affected or unaffected based on their click ABR. SNP identity is indicated on the left and chromosomal location is indicated on the right. The number of mice with each haplotype is indicated below. SNP locations were taken from Genome Reference Consortium Mouse Build 38 patch release 1 (GRCm38.p1). (C) DNA sequence of Aspa exon 3 in +/+ (upper panel) and deaf14/deaf14 mouse (lower panel). A c.516T>A mutation (*) is predicted to encode a p.Y172X premature termination of translation in the deaf14/deaf14 mouse. (D) Western blot of p26 whole brain lysates probed with anti-ASPA (upper panel) and anti-actin (lower panel) antibodies. The upper panel shows a band at 35 kDa, the predicted molecular weight of ASPA, in Aspa+/+ and Aspa+/deaf14 brain that is not present in Aspadeaf14/deaf14 brain. The faint band in the Aspadeaf14/deaf14 lane (*) is of a slightly higher molecular weight and is also present in the two other lanes, albeit almost obscured by the ASPA band. This is likely to be a non-specific band. The lower panel demonstrates that equal amounts of protein were loaded in each lane. NS, non-specific band; 14, deaf14.

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