AB97454

Anti-ASPA antibody

Name: Anti-ASPA antibody (ab97454) | Abcam
Brand: Abcam Limited
SKU: AB97454
Price: 450 USD
Availability: InStock

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(3 Publications)

Anti-ASPA antibody (ab97454) is a rabbit polyclonal antibody detecting ASPA in Western Blot, IHC-P. Suitable for Human.

- Trusted since 2010

View Alternative Names

ACY2, ASP, ASPA, Aspartoacylase, Aminoacylase-2, ACY-2

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASPA antibody (AB97454)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASPA antibody (AB97454)

ab97454 at 1/100 dilution staining ASPA in paraffin-embedded Cal27 xenograft by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

Immunocytochemistry/ Immunofluorescence - Anti-ASPA antibody (AB97454)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ASPA antibody (AB97454)

Immunofluorescence analysis of ICC-IF HeLa cells fixed in 4% paraformaldehyde at RT for 15 min labelling Aspartoacylase protein at cytoplasm using ab97454 at a 1/500 dilution (Green). alpha Tubulin, a cytoskeleton marker was stained at a 1/1000 dilution (Red). Hoechst 33342 was used for nuclear staining (Blue).

Supplier Data

Western blot - Anti-ASPA antibody (AB97454)

Samples were separated by 12% SDS-PAGE.

All lanes:

Western blot - Anti-ASPA antibody (ab97454) at 1/1000 dilution

Lane 1:

U87-MG whole cell extracts at 30 µg

Lane 2:

SK-N-SH whole cell extracts at 30 µg

Lane 3:

IMR32 whole cell extracts at 30 µg

Lane 4:

SK-N-AS whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 36 kDa

false

Supplier Data

Western blot - Anti-ASPA antibody (AB97454)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-ASPA antibody (ab97454) at 1/10000 dilution

Lane 1:

Non-transfected 293T whole cell extracts at 30 µg

Lane 2:

Transfected 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 36 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human ASPA aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

P45381

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/20000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "" }, "Cow": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-ASPA antibody (ab97454) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human samples.

What is the molecular weight of ASPA?
Anti-ASPA (ab97454) specifically detects a band for ASPA (UniProt: P45381) at a molecular weight of 36kDa.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Aspartoacylase also known by its abbreviation ASPA is an enzyme that breaks down N-acetyl-L-aspartate into aspartate and acetate. It has a mass of about 60 kDa. ASPA is highly expressed in the brain especially in oligodendrocytes which are a type of central nervous system cell responsible for producing the myelin sheath that surrounds nerve cells. Its enzymatic activity is dependent on divalent metal ions which are required for its function.

Biological function summary

ASPA plays an important role in myelin sheath maintenance and acetate metabolism. This protein doesn't form part of a complex but acts independently in the cytoplasm and peroxisomes of cells. Its function is significant for the provision of acetate for lipid synthesis which is necessary for myelin production. By regulating N-acetyl-L-aspartate levels in the brain ASPA activity is linked directly to protecting nerve cells' structural integrity.

Pathways

The ASPA enzyme is involved in the aspartate and acetyl-CoA pathways which are central to cellular energy balance and lipid metabolism. Its enzymatic role facilitates the recycling of acetate which is vital to the synthesis of myelin lipids. The relationship of ASPA with enzymes like acetyl-CoA synthetase highlights its involvement in broader biochemical pathways fundamental to cell metabolism and growth.

Deficiencies in ASPA are linked to Canavan disease a rare and inherited neurodegenerative condition characterized by white matter sponginess and loss of motor skills. This disorder results from mutations in the ASPA gene leading to dysfunctional enzyme activity. Additionally changes in ASPA expression levels might contribute to demyelinating disorders where proteins like myelin basic protein are also often involved making ASPA an important target for studies exploring nervous system diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the deacetylation of N-acetylaspartic acid (NAA) to produce acetate and L-aspartate. NAA occurs in high concentration in brain and its hydrolysis NAA plays a significant part in the maintenance of intact white matter. In other tissues it acts as a scavenger of NAA from body fluids.

See full target information ASPA

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cellular and molecular life sciences : CMLS 81:346 PubMed39134808

2024

Low intensity repetitive transcranial magnetic stimulation enhances remyelination by newborn and surviving oligodendrocytes in the cuprizone model of toxic demyelination.

Applications

Unspecified application

Species

Unspecified reactive species

Phuong Tram Nguyen,Kalina Makowiecki,Thomas S Lewis,Alastair J Fortune,Mackenzie Clutterbuck,Laura A Reale,Bruce V Taylor,Jennifer Rodger,Carlie L Cullen,Kaylene M Young

Frontiers in cell and developmental biology 8:564351 PubMed33282858

2020

Low-Density Lipoprotein Receptor-Related Protein 1 (LRP1) Is a Negative Regulator of Oligodendrocyte Progenitor Cell Differentiation in the Adult Mouse Brain.

Applications

Unspecified application

Species

Unspecified reactive species

Loic Auderset,Kimberley A Pitman,Carlie L Cullen,Renee E Pepper,Bruce V Taylor,Lisa Foa,Kaylene M Young

JCI insight 2:e90807 PubMed28194442

2017

Redirecting -acetylaspartate metabolism in the central nervous system normalizes myelination and rescues Canavan disease.

Applications

Unspecified application

Species

Unspecified reactive species

Dominic J Gessler,Danning Li,Hongxia Xu,Qin Su,Julio Sanmiguel,Serafettin Tuncer,Constance Moore,Jean King,Reuben Matalon,Guangping Gao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-ASPA antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASPA antibody (AB97454)

Immunocytochemistry/ Immunofluorescence - Anti-ASPA antibody (AB97454)

Western blot - Anti-ASPA antibody (AB97454)

Western blot - Anti-ASPA antibody (AB97454)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise