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Rabbit Recombinant Monoclonal ASPA antibody. Suitable for IP, WB, IHC-Fr, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 2 publications.

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Images

Western blot - Anti-ASPA antibody [EPR22072] (AB223269), expandable thumbnail
  • Immunoprecipitation - Anti-ASPA antibody [EPR22072] (AB223269), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASPA antibody [EPR22072] (AB223269), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-ASPA antibody [EPR22072] (AB223269), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASPA antibody [EPR22072] (AB223269), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-FrIHC-P
Human
Tested
Tested
Expected
Tested
Mouse
Expected
Tested
Tested
Tested
Rat
Expected
Tested
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/30
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/100
Notes

Perform heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Species
Rat
Dilution info
1/100
Notes

Perform heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Catalyzes the deacetylation of N-acetylaspartic acid (NAA) to produce acetate and L-aspartate. NAA occurs in high concentration in brain and its hydrolysis NAA plays a significant part in the maintenance of intact white matter. In other tissues it acts as a scavenger of NAA from body fluids.

Alternative names

ACY2, ASP, ASPA, Aspartoacylase, Aminoacylase-2, ACY-2

Recommended products

Rabbit Recombinant Monoclonal ASPA antibody. Suitable for IP, WB, IHC-Fr, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 2 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR22072
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Aspartoacylase also known by its abbreviation ASPA is an enzyme that breaks down N-acetyl-L-aspartate into aspartate and acetate. It has a mass of about 60 kDa. ASPA is highly expressed in the brain especially in oligodendrocytes which are a type of central nervous system cell responsible for producing the myelin sheath that surrounds nerve cells. Its enzymatic activity is dependent on divalent metal ions which are required for its function.

Biological function summary

ASPA plays an important role in myelin sheath maintenance and acetate metabolism. This protein doesn't form part of a complex but acts independently in the cytoplasm and peroxisomes of cells. Its function is significant for the provision of acetate for lipid synthesis which is necessary for myelin production. By regulating N-acetyl-L-aspartate levels in the brain ASPA activity is linked directly to protecting nerve cells' structural integrity.

Pathways

The ASPA enzyme is involved in the aspartate and acetyl-CoA pathways which are central to cellular energy balance and lipid metabolism. Its enzymatic role facilitates the recycling of acetate which is vital to the synthesis of myelin lipids. The relationship of ASPA with enzymes like acetyl-CoA synthetase highlights its involvement in broader biochemical pathways fundamental to cell metabolism and growth.

Associated diseases and disorders

Deficiencies in ASPA are linked to Canavan disease a rare and inherited neurodegenerative condition characterized by white matter sponginess and loss of motor skills. This disorder results from mutations in the ASPA gene leading to dysfunctional enzyme activity. Additionally changes in ASPA expression levels might contribute to demyelinating disorders where proteins like myelin basic protein are also often involved making ASPA an important target for studies exploring nervous system diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Western blot - Anti-ASPA antibody [EPR22072] (ab223269), expandable thumbnail

    Western blot - Anti-ASPA antibody [EPR22072] (ab223269)

    Exposure time : Lanes 1-3: 15 seconds; Lanes 4-6: 8 seconds.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-ASPA antibody [EPR22072] (ab223269) at 1/1000 dilution

    Lane 1: Human brain lysate at 20 µg

    Lane 2: Human fetal kidney lysate at 20 µg

    Lane 3: Human testis lysate at 20 µg

    Lane 4: Mouse brain lysate at 20 µg

    Lane 5: Rat brain lysate at 20 µg

    Lane 6: Rat testis lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 36 kDa

    Observed band size: 36 kDa

  • Immunoprecipitation - Anti-ASPA antibody [EPR22072] (ab223269), expandable thumbnail

    Immunoprecipitation - Anti-ASPA antibody [EPR22072] (ab223269)

    ASPA was immunoprecipitated from 0.35 mg human brain lysate with ab223269 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab223269 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/5000 dilution.

    Lane 1: Human brain lysate 10 μg (Input).
    Lane 2: ab223269 IP in human brain lysate.
    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab223269 in human brain lysate.

    Blocking/Dilution buffer: 5% NFDM/TBST.
    Exposure time: 10 seconds.

    All lanes: Immunoprecipitation - Anti-ASPA antibody [EPR22072] (ab223269)

    Predicted band size: 36 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASPA antibody [EPR22072] (ab223269), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASPA antibody [EPR22072] (ab223269)

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling ASPA with ab223269 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear and cytoplasmic staining on gliocytes of mouse cerebrum (PMID: 29116375). Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Frozen sections) - Anti-ASPA antibody [EPR22072] (ab223269), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-ASPA antibody [EPR22072] (ab223269)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen rat cerebrum tissue labeling ASPA with ab223269 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Nuclear and cytoplasmic staining on gliocytes of rat cerebrum. (PMID: 29116375).

    The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASPA antibody [EPR22072] (ab223269), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASPA antibody [EPR22072] (ab223269)

    Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue labeling ASPA with ab223269 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear and cytoplasmic staining on gliocytes of rat cerebral cortex (PMID: 29116375). Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASPA antibody [EPR22072] (ab223269), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASPA antibody [EPR22072] (ab223269)

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling ASPA with ab223269 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear and cytoplasmic staining on gliocytes of mouse cerebrum (PMID: 29116375). Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASPA antibody [EPR22072] (ab223269), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASPA antibody [EPR22072] (ab223269)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling ASPA with ab223269 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear and cytoplasmic staining mainly on proximal renal tubules of human kidney (PMID: 16935940). Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASPA antibody [EPR22072] (ab223269), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASPA antibody [EPR22072] (ab223269)

    Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling ASPA with ab223269 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear and cytoplasmic staining on gliocytes of human cerebrum (PMID: 29116375). Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Frozen sections) - Anti-ASPA antibody [EPR22072] (ab223269), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-ASPA antibody [EPR22072] (ab223269)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebrum tissue labeling ASPA with ab223269 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Nuclear and cytoplasmic staining on gliocytes of mouse cerebrum (PMID: 29116375).

    The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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