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AB126254

Anti-Asparagine synthetase antibody

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(3 Publications)

Rabbit Polyclonal Asparagine synthetase antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ASNS aa 1-250.

View Alternative Names

TS11, ASNS, Asparagine synthetase [glutamine-hydrolyzing], Cell cycle control protein TS11, Glutamine-dependent asparagine synthetase

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Asparagine synthetase antibody (AB126254)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Asparagine synthetase antibody (AB126254)

ab126254, at 1/500, staining Asparagine synthetase in Human Cal27 xenograftby immunohistochemistry [Paraffin Embedded Tissues (IHC-P).

Western blot - Anti-Asparagine synthetase antibody (AB126254)
  • WB

Unknown

Western blot - Anti-Asparagine synthetase antibody (AB126254)

Gel concentration : 7.5%

All lanes:

Western blot - Anti-Asparagine synthetase antibody (ab126254) at 1/1000 dilution

All lanes:

K562 whole cell lysate at 30 µg

Predicted band size: 64 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human ASNS aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

P08243

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Asparagine synthetase (ASNS) is an enzyme that catalyzes the conversion of aspartate and glutamine to asparagine and glutamate. This enzyme is also known by the alternate name ASPG. Asparagine synthetase weighs approximately 64 kDa and it is found in many tissues including the liver and pancreas. The presence of ASNS is important for the synthesis of asparagine an amino acid necessary for protein and nucleotide synthesis.
Biological function summary

ASNS drives the synthesis of asparagine through a mechanism involving the ATP-dependent conversion of substrates. It does not function as part of a larger enzyme complex but operates independently to fulfill its role. In cells asparagine produced serves as a critical building block supporting protein biosynthesis and cell proliferation particularly in rapidly dividing cells such as cancer cells.

Pathways

Asparagine synthetase plays a central role in the asparagine biosynthetic pathway. This enzyme is key for maintaining cellular amino acid homeostasis and is linked to the mTOR pathway which regulates cell growth and metabolism. Additionally ASNS interacts with proteins like glutaminase which supplies one of its essential substrates glutamine.

ASNS has a significant correlation with acute lymphoblastic leukemia (ALL) where asparagine levels affect cancer cell survival. Targeting ASNS can influence chemotherapeutic strategies given its role in providing asparagine to leukemic cells. Additionally ASNS deficiency can lead to severe neurological conditions further highlighting its importance in human health. Its interaction with glutaminase is particularly relevant as both are involved in pathways targeted in ALL treatments.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information ASNS
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

EBioMedicine 72:103614 PubMed34628354

2021

SLC1A1 mediated glutamine addiction and contributed to natural killer T-cell lymphoma progression with immunotherapeutic potential.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Xiong,Nan Wang,Hui-Juan Zhong,Bo-Wen Cui,Shu Cheng,Rui Sun,Jia-Yi Chen,Peng-Peng Xu,Gang Cai,Li Wang,Xiao-Jian Sun,Jin-Yan Huang,Wei-Li Zhao

Cancer & metabolism 8:23 PubMed33101674

2020

Metabolic plasticity of IDH1 glioma cell lines is responsible for low sensitivity to glutaminase inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Victor Ruiz-Rodado,Adrian Lita,Tyrone Dowdy,Orieta Celiku,Alejandra Cavazos Saldana,Herui Wang,Chun Zhang Yang,Raj Chari,Aiguo Li,Wei Zhang,Hua Song,Meili Zhang,Susie Ahn,Dionne Davis,Xiang Chen,Zhengping Zhuang,Christel Herold-Mende,Kylie J Walters,Mark R Gilbert,Mioara Larion

Cell death & disease 10:239 PubMed30858360

2019

SOX12 promotes colorectal cancer cell proliferation and metastasis by regulating asparagine synthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Du,Jie Chen,Hao Liu,Yanhui Cai,Tianyu Cao,Weili Han,Xiaofang Yi,Meirui Qian,Dean Tian,Yongzhan Nie,Kaichun Wu,Daiming Fan,Limin Xia
View all publications
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Product promise

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