AB189863

Anti-Aspartate Aminotransferase antibody - C-terminal

Name: Anti-Aspartate Aminotransferase antibody - C-terminal (ab189863) | Abcam
Brand: Abcam Limited
SKU: AB189863
Price: 535 USD
Availability: InStock

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(5 Publications)

Rabbit Polyclonal Aspartate Aminotransferase antibody. C-terminal. Suitable for WB and reacts with Human, Mouse samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Aspartate aminotransferase, cytoplasmic aa 200 to C-terminus.

View Alternative Names

cAspAT, Glutamate oxaloacetate transaminase 1, Transaminase A, cCAT, GOT1

1 Images

Supplier Data

Western blot - Anti-Aspartate Aminotransferase antibody - C-terminal (AB189863)

All lanes:

Western blot - Anti-Aspartate Aminotransferase antibody - C-terminal (ab189863) at 1/500 dilution

Lane 1:

Jurkat cell extract

Lane 2:

293T cell extract

Lane 3:

HepG2 cell extract

Lane 4:

A549 cell extract

Lane 5:

HeLa cell extract

Lane 6:

mouse liver extract

Lane 7:

mouse kidney extract

Lane 8:

mouse heart extract

Predicted band size: 46 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

applications

Immunogen

Recombinant Fragment Protein within Human Aspartate aminotransferase, cytoplasmic aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P17174

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Reactivity data

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Aspartate Aminotransferase (AST) also known as aspartate transaminase or aspartate transferase is an enzyme important for amino acid metabolism. Mechanically it catalyzes the reversible transfer of an amino group from aspartate to α-ketoglutarate forming oxaloacetate and glutamate. AST has a molecular mass of approximately 92 kDa and is expressed in various tissues with high levels found in liver heart muscle and kidneys. Its presence in these tissues highlights its importance in cellular metabolic processes.

Biological function summary

AST facilitates the interconversion between aspartate and oxaloacetate playing a role in the amino acid and urea cycles. Though not part of a large complex AST works closely with similar enzymes such as alanine aminotransferase (ALT) to maintain amino acid balance and support energy production. This enzymatic activity is important in nitrogen metabolism and glutamate use which are necessary for synthesizing other essential molecules within the cell.

Pathways

AST participates in the citric acid cycle and malate-aspartate shuttle enabling efficient energy production and NADH transport. The enzyme assists in converting oxaloacetate a pivotal intermediate in the citric acid cycle to keep the cycle active ensuring efficient cellular respiration. Additionally in the malate-aspartate shuttle AST works alongside malate dehydrogenase to facilitate the transfer of reducing equivalents across the mitochondrial membrane which is essential for ATP generation.

Elevated AST levels often indicate liver damage such as in hepatitis or cirrhosis reflecting its significant tissue expression. The enzyme is also linked to myocardial infarction as damaged heart muscle releases AST into the bloodstream. In these conditions AST serves as a biomarker for tissue damage often in conjunction with ALT levels allowing clinicians to assess the extent of liver or cardiac injury.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Biosynthesis of L-glutamate from L-aspartate or L-cysteine (PubMed : 21900944). Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H(2)S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain. In addition, catalyzes (2S)-2-aminobutanoate, a by-product in the cysteine biosynthesis pathway (PubMed : 27827456).

See full target information Aspartate aminotransferase, cytoplasmic

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:2088 PubMed40025024

2025

In situ valence-transited arsenic nanosheets for multi-modal therapy of colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Hongyue Zheng,Ke Zhang,Jigang Piao,Chaofeng Mu,Xiaowei Xie,Mengying Cheng,Tianxiang Yue,Jiang Sun,Bin Li,Yinghui Wei,Hangsheng Zheng,Lai Jiang,Douae Nihed Habiballah,Fanzhu Li

International journal of urology : official journal of the Japanese Urological Association 28:459-465 PubMed33403726

2021

Age-related differences in responses to hydrogen sulfide in the bladder of spontaneously hypertensive rats.

Applications

Unspecified application

Species

Unspecified reactive species

Suo Zou,Takahiro Shimizu,Masaki Yamamoto,Shogo Shimizu,Youichirou Higashi,Takashi Karashima,Motoaki Saito

Journal of molecular and cellular cardiology 138:304-317 PubMed31836543

2019

Defining decreased protein succinylation of failing human cardiac myofibrils in ischemic cardiomyopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Hadi R Ali,Cole R Michel,Ying H Lin,Timothy A McKinsey,Mark Y Jeong,Amrut V Ambardekar,Joseph C Cleveland,Richard Reisdorph,Nichole Reisdorph,Kathleen C Woulfe,Kristofer S Fritz

Journal of proteome research 18:1513-1531 PubMed30644754

2019

Quantifying Competition among Mitochondrial Protein Acylation Events Induced by Ethanol Metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Hadi R Ali,Mohammed A Assiri,Peter S Harris,Cole R Michel,Youngho Yun,John O Marentette,Frank K Huynh,David J Orlicky,Colin T Shearn,Laura M Saba,Richard Reisdorph,Nichole Reisdorph,Matthew D Hirschey,Kristofer S Fritz

Cell metabolism 28:817-832.e8 PubMed30244971

2018

Translational and HIF-1α-Dependent Metabolic Reprogramming Underpin Metabolic Plasticity and Responses to Kinase Inhibitors and Biguanides.

Applications

Unspecified application

Species

Unspecified reactive species

Laura Hulea,Simon-Pierre Gravel,Masahiro Morita,Marie Cargnello,Oro Uchenunu,Young Kyuen Im,Camille Lehuédé,Eric H Ma,Matthew Leibovitch,Shannon McLaughlan,Marie-José Blouin,Maxime Parisotto,Vasilios Papavasiliou,Cynthia Lavoie,Ola Larsson,Michael Ohh,Tiago Ferreira,Celia Greenwood,Gaëlle Bridon,Daina Avizonis,Gerardo Ferbeyre,Peter Siegel,Russell G Jones,William Muller,Josie Ursini-Siegel,Julie St-Pierre,Michael Pollak,Ivan Topisirovic

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Aspartate Aminotransferase antibody - C-terminal

Western blot - Anti-Aspartate Aminotransferase antibody - C-terminal (AB189863)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise