Rabbit Recombinant Monoclonal Aspartate Aminotransferase antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 9 publications.
IgG
Rabbit
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
WB | ICC/IF | Flow Cyt (Intra) | IHC-P | |
---|---|---|---|---|
Human | Tested | Tested | Tested | Tested |
Mouse | Tested | Expected | Expected | Expected |
Rat | Tested | Expected | Expected | Expected |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/3000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info 1/3000 | Notes - |
Species Rat | Dilution info 1/3000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/500 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/20 | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/170 | Notes The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
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Biosynthesis of L-glutamate from L-aspartate or L-cysteine (PubMed:21900944). Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H(2)S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain. In addition, catalyzes (2S)-2-aminobutanoate, a by-product in the cysteine biosynthesis pathway (PubMed:27827456).
Aspartate aminotransferase, mitochondrial
cAspAT, Glutamate oxaloacetate transaminase 1, Transaminase A, cCAT, GOT1
Rabbit Recombinant Monoclonal Aspartate Aminotransferase antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 9 publications.
IgG
Rabbit
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
EPR12145
Affinity purification Protein A
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
This supplementary information is collated from multiple sources and compiled automatically.
Aspartate Aminotransferase also known as AST or alkaline aminotransferase is an important enzyme in amino acid metabolism. It catalyzes the transfer of an amino group from aspartate to alpha-ketoglutarate forming glutamate and oxaloacetate. This enzyme has an approximate molecular mass of 46 kDa and is expressed in the liver heart skeletal muscle and kidney tissues. AST plays a significant role in the intracellular balance of amino acids and is an important biomarker in clinical diagnostics.
AST functions in the transamination process a mechanism central to the synthesis and degradation of amino acids. This process is fundamental in the production of energy and the maintenance of the urea cycle. AST does not form a part of a complex but interacts dynamically with substrates and cofactors like pyridoxal phosphate essential for its enzymatic activity. The activity of AST is important in ensuring the proper function of amino acid metabolism in various tissues.
AST operates within the citric acid cycle and the urea cycle. In the citric acid cycle AST contributes to the interconversion of amino acids and metabolic intermediates aiding in energy production. It links to proteins such as malate dehydrogenase and glutamate dehydrogenase which play roles in associated metabolic conversions and energy cycles. These interactions reflect AST's integration in maintaining cellular metabolic processes.
AST levels serve as an indicator of liver health and are often elevated in conditions such as hepatitis and myocardial infarction. Hepatitis causes liver damage resulting in increased AST release into the bloodstream. In myocardial infarction damaged cardiac tissue releases AST providing a diagnostic cue for tissue injury. Elevated AST levels can associate with other enzymes like alanine aminotransferase (ALT) used together to assess liver and heart function.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Blocking and diluting buffer: 5% NFDM/TBST.
All lanes: purified at 1/3000 dilution
Lane 1: HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates at 20 µg
Lane 2: MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates at 20 µg
Lane 3: K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates at 20 µg
Lane 4: Mouse brain lysates at 20 µg
Lane 5: Mouse heart lysates at 20 µg
Lane 6: Rat brain lysates at 20 µg
Lane 7: Rat heart lysates at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
All lanes: Western blot - Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] (ab170950) at 1/1000 dilution
All lanes: Western blot - Recombinant human FABP-1 protein (Recombinant human FABP-1 protein ab206788) at 0.015 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 47 kDa
Exposure time: 180s
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170950).
Immunocytochemistry/Immunofluorescence analysis of HT-29 (human colorectal adenocarcinoma) cells labelling Aspartate Aminotransferase + FABP-1 with purified ab170950 at 1/120. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution was used as the secondary antibody. Nuclei counterstained with DAPI (blue).
Secondary Only Control: PBS was used instead of the primary antibody as the negative control.
Intracellular Flow Cytometry analysis of K-562 (Human chronic myelogenous leukemia lymphoblast) cells labeling Aspartate Aminotransferase + FABP-1 with purified ab170950 at 1/20 dilution (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human glioma tissue sections labeling Aspartate Aminotransferase + FABP-1 with Purified ab170950 at 1:170 dilution. Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling Aspartate Aminotransferase + FABP-1 using unpurified ab170950 at 1/50 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
All lanes: Western blot - Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] (ab170950) at 1/1000 dilution
Lane 1: HepG2 cell lysate at 10 µg
Lane 2: MCF-7 cell lysate at 10 µg
Lane 3: K562 cell lysate at 10 µg
Immunofluorescent analysis of HepG2 cells labeling Aspartate Aminotransferase + FABP-1 using unpurified ab170950 at 1/50 dilution (green). DAPI nuclear staining (blue).
Intracellular flow cytometric analysis of permeabilized K562 cells labeling Aspartate Aminotransferase + FABP-1 using unpurified ab170950 at 1/10 dilution (red) or a rabbit IgG negative (green).
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