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Rabbit Recombinant Monoclonal Aspartate Aminotransferase antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 9 publications.


Images

Western blot - Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] (AB170950), expandable thumbnail
  • Western blot - Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] (AB170950), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] (AB170950), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] (AB170950), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] (AB170950), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFFlow Cyt (Intra)IHC-P
Human
Tested
Tested
Tested
Tested
Mouse
Tested
Expected
Expected
Expected
Rat
Tested
Expected
Expected
Expected

Tested
Tested

Species

Human

Dilution info

1/3000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Mouse

Dilution info

1/3000

Notes

-

Species

Rat

Dilution info

1/3000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/500

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1/20

Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1/170

Notes

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

6 products for Alternative Product

Target data

Function

Biosynthesis of L-glutamate from L-aspartate or L-cysteine (PubMed:21900944). Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H(2)S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain. In addition, catalyzes (2S)-2-aminobutanoate, a by-product in the cysteine biosynthesis pathway (PubMed:27827456).

Targets

Aspartate aminotransferase, mitochondrial

Alternative names

Recommended products

Rabbit Recombinant Monoclonal Aspartate Aminotransferase antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 9 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR12145

Purification technique

Affinity purification Protein A

Specificity

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Aspartate Aminotransferase also known as AST or alkaline aminotransferase is an important enzyme in amino acid metabolism. It catalyzes the transfer of an amino group from aspartate to alpha-ketoglutarate forming glutamate and oxaloacetate. This enzyme has an approximate molecular mass of 46 kDa and is expressed in the liver heart skeletal muscle and kidney tissues. AST plays a significant role in the intracellular balance of amino acids and is an important biomarker in clinical diagnostics.

Biological function summary

AST functions in the transamination process a mechanism central to the synthesis and degradation of amino acids. This process is fundamental in the production of energy and the maintenance of the urea cycle. AST does not form a part of a complex but interacts dynamically with substrates and cofactors like pyridoxal phosphate essential for its enzymatic activity. The activity of AST is important in ensuring the proper function of amino acid metabolism in various tissues.

Pathways

AST operates within the citric acid cycle and the urea cycle. In the citric acid cycle AST contributes to the interconversion of amino acids and metabolic intermediates aiding in energy production. It links to proteins such as malate dehydrogenase and glutamate dehydrogenase which play roles in associated metabolic conversions and energy cycles. These interactions reflect AST's integration in maintaining cellular metabolic processes.

Associated diseases and disorders

AST levels serve as an indicator of liver health and are often elevated in conditions such as hepatitis and myocardial infarction. Hepatitis causes liver damage resulting in increased AST release into the bloodstream. In myocardial infarction damaged cardiac tissue releases AST providing a diagnostic cue for tissue injury. Elevated AST levels can associate with other enzymes like alanine aminotransferase (ALT) used together to assess liver and heart function.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Western blot - Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] (ab170950), expandable thumbnail

    Western blot - Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] (ab170950)

    Blocking and diluting buffer: 5% NFDM/TBST.

    All lanes: purified at 1/3000 dilution

    Lane 1: HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates at 20 µg

    Lane 2: MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates at 20 µg

    Lane 3: K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates at 20 µg

    Lane 4: Mouse brain lysates at 20 µg

    Lane 5: Mouse heart lysates at 20 µg

    Lane 6: Rat brain lysates at 20 µg

    Lane 7: Rat heart lysates at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

  • Western blot - Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] (ab170950), expandable thumbnail

    Western blot - Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] (ab170950)

    All lanes: Western blot - Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] (ab170950) at 1/1000 dilution

    All lanes: Western blot - Recombinant human FABP-1 protein (Recombinant human FABP-1 protein ab206788) at 0.015 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 47 kDa

    Exposure time: 180s

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170950).

  • Immunocytochemistry/ Immunofluorescence - Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] (ab170950), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] (ab170950)

    Immunocytochemistry/Immunofluorescence analysis of HT-29 (human colorectal adenocarcinoma) cells labelling Aspartate Aminotransferase + FABP-1 with purified ab170950 at 1/120. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution was used as the secondary antibody. Nuclei counterstained with DAPI (blue).

    Secondary Only Control: PBS was used instead of the primary antibody as the negative control.

  • Flow Cytometry (Intracellular) - Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] (ab170950), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] (ab170950)

    Intracellular Flow Cytometry analysis of K-562 (Human chronic myelogenous leukemia lymphoblast) cells labeling Aspartate Aminotransferase + FABP-1 with purified ab170950 at 1/20 dilution (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] (ab170950), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] (ab170950)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human glioma tissue sections labeling Aspartate Aminotransferase + FABP-1 with Purified ab170950 at 1:170 dilution. Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] (ab170950), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] (ab170950)

    Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling Aspartate Aminotransferase + FABP-1 using unpurified ab170950 at 1/50 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] (ab170950), expandable thumbnail

    Western blot - Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] (ab170950)

    All lanes: Western blot - Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] (ab170950) at 1/1000 dilution

    Lane 1: HepG2 cell lysate at 10 µg

    Lane 2: MCF-7 cell lysate at 10 µg

    Lane 3: K562 cell lysate at 10 µg

  • Immunocytochemistry/ Immunofluorescence - Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] (ab170950), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] (ab170950)

    Immunofluorescent analysis of HepG2 cells labeling Aspartate Aminotransferase + FABP-1 using unpurified ab170950 at 1/50 dilution (green). DAPI nuclear staining (blue).

  • Flow Cytometry (Intracellular) - Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] (ab170950), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] (ab170950)

    Intracellular flow cytometric analysis of permeabilized K562 cells labeling Aspartate Aminotransferase + FABP-1 using unpurified ab170950 at 1/10 dilution (red) or a rabbit IgG negative (green).

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Product protocols

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