Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] - BSA and Azide free

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] - BSA and Azide free (AB232471)

Intracellular Flow Cytometry analysis of K-562 (Human chronic myelogenous leukemia lymphoblast) cells labeling Aspartate Aminotransferase + FABP-1 with purified ab170950 at 1/20 dilution (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor^® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170950).

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] - BSA and Azide free (AB232471)

Immunocytochemistry/Immunofluorescence analysis of HT-29 (human colorectal adenocarcinoma) cells labelling Aspartate Aminotransferase + FABP-1 with purified ab170950 at 1/120. Cells were fixed with 100% methanol. An Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (ab150077) at 1/1000 dilution was used as the secondary antibody. Nuclei counterstained with DAPI (blue).

Secondary Only Control : PBS was used instead of the primary antibody as the negative control.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170950).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] - BSA and Azide free (AB232471)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human glioma tissue sections labeling Aspartate Aminotransferase + FABP-1 with Purified ab170950 at 1 : 170 dilution. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1 : 0 dilution. PBS instead of the primary antibody was used as the negative control.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170950).

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] - BSA and Azide free (AB232471)

Intracellular flow cytometric analysis of permeabilized K562 cells labeling Aspartate Aminotransferase + FABP-1 using unpurified ab170950 at 1/10 dilution (red) or a rabbit IgG negative (green).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170950).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] - BSA and Azide free (AB232471)

Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling Aspartate Aminotransferase + FABP-1 using unpurified ab170950 at 1/50 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170950).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] - BSA and Azide free (AB232471)

Immunofluorescent analysis of HepG2 cells labeling Aspartate Aminotransferase + FABP-1 using unpurified ab170950 at 1/50 dilution (green). DAPI nuclear staining (blue).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170950).

• WB

Unknown

Western blot - Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] - BSA and Azide free (AB232471)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab170950).

All lanes:

Western blot - Anti-Aspartate Aminotransferase + FABP-1 antibody [EPR12145] (<a href='/en-us/products/primary-antibodies/aspartate-aminotransferase-fabp-1-antibody-epr12145-ab170950'>ab170950</a>) at 1/1000 dilution

All lanes:

Western blot - Recombinant human FABP-1 protein (His tag N-Terminus) (<a href='/en-us/products/proteins-peptides/recombinant-human-fabp-1-protein-ab206788'>ab206788</a>) at 0.015 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 47 kDa

false

Exposure time: 180s