AB154805

Anti-Aspartyl Aminopeptidase antibody [EPR10301]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal Aspartyl Aminopeptidase antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 2 publications.

View Alternative Names

ASPEP, DAP, DNPEP, Aspartyl aminopeptidase

2 Images

Flow Cytometry (Intracellular) - Anti-Aspartyl Aminopeptidase antibody [EPR10301] (AB154805)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Aspartyl Aminopeptidase antibody [EPR10301] (AB154805)

Intracellular flow cytometric analysis of permeabilized 293T cells staining Aspartyl Aminopeptidase using ab154805 at a 1/10 dilution (red) or a rabbit IgG (negative) (green).

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Western blot - Anti-Aspartyl Aminopeptidase antibody [EPR10301] (AB154805)

All lanes:

Western blot - Anti-Aspartyl Aminopeptidase antibody [EPR10301] (ab154805) at 1/1000 dilution

Lane 1:

MCF7 lysate at 10 µg

Lane 2:

293T lysate at 10 µg

Lane 3:

K562 lysate at 10 µg

Lane 4:

A549 lysate at 10 µg

Predicted band size: 52 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10301

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Aspartyl Aminopeptidase also known as DNPEP is a metallopeptidase enzyme that mechanically cleaves N-terminal aspartic acid residues from peptides. This enzyme has a molecular mass of about 52 kDa. Aspartyl Aminopeptidase is expressed in various tissues with high abundance in liver and kidney. Its subcellular location is mainly the cytosol where it is involved in protein processing and turnover.

Biological function summary

Aspastyl aminopeptidase plays a role in peptide metabolism by trimming aspartic acid residues aiding in protein degradation and maturation. It functions independently and is not known to be part of a larger protein complex. Its activity facilitates the maintenance of normal peptide levels which is essential for various cellular processes.

Pathways

Aspartyl aminopeptidase is involved in the renin-angiotensin system an important regulator of blood pressure and fluid balance. Within this pathway it modulates levels of angiotensin peptides. It also interacts with other proteins such as angiotensin-converting enzyme (ACE) and neprilysin highlighting its importance in cardiovascular homeostasis.

Research links aspartyl aminopeptidase to essential hypertension due to its role in the renin-angiotensin system. Abnormal activity of this enzyme might contribute to alterations in angiotensin metabolism therefore impacting blood pressure regulation. Additionally dysregulation may have implications in kidney diseases where interaction with ACE affects renal function and blood pressure control.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Aminopeptidase with specificity towards an acidic amino acid at the N-terminus. Likely to play an important role in intracellular protein and peptide metabolism.

See full target information DNPEP

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Physiological reports 5: PubMed29122955

2017

DNPEP is not the only peptidase that produces SPAK fragments in kidney.

Applications

Unspecified application

Species

Unspecified reactive species

Rainelli Koumangoye,Eric Delpire

The Journal of biological chemistry 289:29273-84 PubMed25164821

2014

Short forms of Ste20-related proline/alanine-rich kinase (SPAK) in the kidney are created by aspartyl aminopeptidase (Dnpep)-mediated proteolytic cleavage.

Applications

Unspecified application

Species

Unspecified reactive species

Nicolas Markadieu,Kerri Rios,Benjamin W Spiller,W Hayes McDonald,Paul A Welling,Eric Delpire

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Aspartyl Aminopeptidase antibody [EPR10301]

Flow Cytometry (Intracellular) - Anti-Aspartyl Aminopeptidase antibody [EPR10301] (AB154805)

Western blot - Anti-Aspartyl Aminopeptidase antibody [EPR10301] (AB154805)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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