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AB249112

Anti-Aspartyl Aminopeptidase antibody [EPR10301] - BSA and Azide free

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Rabbit Recombinant Monoclonal Aspartyl Aminopeptidase antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

ASPEP, DAP, DNPEP, Aspartyl aminopeptidase

1 Images
Western blot - Anti-Aspartyl Aminopeptidase antibody [EPR10301] - BSA and Azide free (AB249112)
  • WB

Unknown

Western blot - Anti-Aspartyl Aminopeptidase antibody [EPR10301] - BSA and Azide free (AB249112)

This data was developed using ab154805, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Aspartyl Aminopeptidase antibody [EPR10301] (<a href='/en-us/products/primary-antibodies/aspartyl-aminopeptidase-antibody-epr10301-ab154805'>ab154805</a>) at 1/1000 dilution

Lane 1:

MCF7 lysate at 10 µg

Lane 2:

293T lysate at 10 µg

Lane 3:

K562 lysate at 10 µg

Lane 4:

A549 lysate at 10 µg

Predicted band size: 52 kDa

false

  • Unconjugated

    Anti-Aspartyl Aminopeptidase antibody [EPR10301]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10301

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

ab249112 is the carrier-free version of ab154805.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Aspartyl Aminopeptidase also known as DNPEP is a metallopeptidase enzyme that mechanically cleaves N-terminal aspartic acid residues from peptides. This enzyme has a molecular mass of about 52 kDa. Aspartyl Aminopeptidase is expressed in various tissues with high abundance in liver and kidney. Its subcellular location is mainly the cytosol where it is involved in protein processing and turnover.
Biological function summary

Aspastyl aminopeptidase plays a role in peptide metabolism by trimming aspartic acid residues aiding in protein degradation and maturation. It functions independently and is not known to be part of a larger protein complex. Its activity facilitates the maintenance of normal peptide levels which is essential for various cellular processes.

Pathways

Aspartyl aminopeptidase is involved in the renin-angiotensin system an important regulator of blood pressure and fluid balance. Within this pathway it modulates levels of angiotensin peptides. It also interacts with other proteins such as angiotensin-converting enzyme (ACE) and neprilysin highlighting its importance in cardiovascular homeostasis.

Research links aspartyl aminopeptidase to essential hypertension due to its role in the renin-angiotensin system. Abnormal activity of this enzyme might contribute to alterations in angiotensin metabolism therefore impacting blood pressure regulation. Additionally dysregulation may have implications in kidney diseases where interaction with ACE affects renal function and blood pressure control.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Aminopeptidase with specificity towards an acidic amino acid at the N-terminus. Likely to play an important role in intracellular protein and peptide metabolism.
See full target information DNPEP
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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