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AB20419

Anti-Aspergillus antibody

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(9 Publications)

Rabbit Polyclonal Aspergillus antibody. Suitable for ELISA, IHC-P, IHC-Fr and reacts with Aspergillus nidulans samples. Cited in 9 publications. Immunogen corresponding to Cell preparation containing Aspergillus protein.
2 Images
Immunohistochemistry (Frozen sections) - Anti-Aspergillus antibody (AB20419)
  • IHC-Fr

PubMed

Immunohistochemistry (Frozen sections) - Anti-Aspergillus antibody (AB20419)

ab20419, staining Aspergillus (green) in lung tissue from a murine model of chemotherapy-induced invasive pulmonary aspergillosis, by Immunohistochemistry.

Tissue was fixed in cold acetone for 15 min, followed by washing and blocking with normal serum block at RT for 30 min. Sections were incubated overnight at 4°C with primary antibody. An AlexaFluor®488-conjugated goat anti-rabbit IgG (1/00) was used as the secondary antibody.

Image from Grahl N et al., PLoS Pathog. 2011 Jul;7(7):e1002145. Epub 2011 Jul 21. Fig 2.; doi:10.1371/journal.ppat.1002145; July 21, 2011, PLoS Pathog 7(7): e1002145.

Immunohistochemistry (Frozen sections) - Anti-Aspergillus antibody (AB20419)
  • IHC-Fr

PubMed

Immunohistochemistry (Frozen sections) - Anti-Aspergillus antibody (AB20419)

ab20419, staining Aspergillus (green) in lung tissue from a murine model of corticosteroid-induced invasive pulmonary aspergillosis, by Immunohistochemistry.

Tissue was fixed in cold acetone for 15 min, followed by washing and blocking with normal serum block at RT for 30 min. Sections were incubated overnight at 4°C with primary antibody. An AlexaFluor®488-conjugated goat anti-rabbit IgG (1/00) was used as the secondary antibody.

Image from Grahl N et al., PLoS Pathog. 2011 Jul;7(7):e1002145. Epub 2011 Jul 21. Fig 2.; doi:10.1371/journal.ppat.1002145; July 21, 2011, PLoS Pathog 7(7): e1002145.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Aspergillus nidulans

Applications

ELISA, IHC-Fr, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Aspergillus nidulans": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
>95% pure
Storage buffer
Preservative: 0.1% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Aspergillus refers to a genus of fungi with over 200 species. It is important in both medical and agricultural contexts. Aspergillus species such as A. fumigatus and A. niger have mechanical roles in decomposition recycling carbon and nutrients. This fungus expresses its components widely in the environment particularly in soil and organic materials. The mass of Aspergillus spores can vary but they are generally lightweight to facilitate airborne distribution. Researchers often study Aspergillus to understand its mechanical role in organic matter breakdown.
Biological function summary

The fungi serve critical roles in ecosystems by breaking down complex organic polymers. Aspergillus can grow and thrive in a variety of conditions and environments including air and soil. It also forms part of microbial communities where it interacts with bacteria and other fungi. Aspergillus works in synergy with various microbial species to degrade plant biomass producing enzymes that further facilitate decomposition processes. Despite its beneficial roles some Aspergillus species like A. flavus produce aflatoxins which are harmful to both humans and animals.

Pathways

With regard to pathways in which the Aspergillus plays a role it is heavily involved in the decomposition pathway for breaking down lignocellulosic biomass. This process involves enzymes that decompose cellulose and hemicellulose into simpler molecules digestible by other organisms. Within these pathways Aspergillus enzymes like cellulases and xylanases collaborate with proteins involved in carbohydrate metabolism. Some species also participate in biosynthetic pathways for secondary metabolites which may contribute to their survival or competition in the environment.

Aspergillus is notably related to aspergillosis which affects individuals with compromised immune systems. Aspergillus fumigatus is the primary culprit in invasive pulmonary aspergillosis. This infection can become serious and sometimes fatal if not promptly treated. Additionally Aspergillus can contribute to allergic reactions as seen in conditions such as asthma and allergic bronchopulmonary aspergillosis (ABPA). During these disorders the specter of a dysfunctional immune response can exacerbate the effects of these pathological states.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Microorganisms 12: PubMed39458334

2024

One-Step Soft Agar Enrichment and Isolation of Human Lung Bacteria Inhibiting the Germination of Conidia.

Applications

Unspecified application

Species

Unspecified reactive species

Fabio Palmieri,Jérémy Diserens,Manon Gresse,Margo Magnin,Julina Helle,Benoît Salamin,Lorenzo Bisanti,Eric Bernasconi,Julie Pernot,Apiha Shanmuganathan,Aurélien Trompette,Christophe von Garnier,Thomas Junier,Samuel Neuenschwander,Saskia Bindschedler,Marco Pagni,Angela Koutsokera,Niki Ubags,Pilar Junier

Pathology international 74:648-654 PubMed39194064

2024

Rare finding of mucorales sporangia and chlamydospores in tissue.

Applications

Unspecified application

Species

Unspecified reactive species

Misa Kojima,Masatomo Kimura,Kazuhiko Kuwahara,Hisatomo Tamaki,Ryuji Yasumatsu,Sota Sadamoto,Takayuki Shinohara,Kazuki Amemiya,Yoshitsugu Miyazaki,Akihiko Ito

Cell 185:3789-3806.e17 PubMed36179670

2022

Pan-cancer analyses reveal cancer-type-specific fungal ecologies and bacteriome interactions.

Applications

Unspecified application

Species

Unspecified reactive species

Lian Narunsky-Haziza,Gregory D Sepich-Poore,Ilana Livyatan,Omer Asraf,Cameron Martino,Deborah Nejman,Nancy Gavert,Jason E Stajich,Guy Amit,Antonio González,Stephen Wandro,Gili Perry,Ruthie Ariel,Arnon Meltser,Justin P Shaffer,Qiyun Zhu,Nora Balint-Lahat,Iris Barshack,Maya Dadiani,Einav N Gal-Yam,Sandip Pravin Patel,Amir Bashan,Austin D Swafford,Yitzhak Pilpel,Rob Knight,Ravid Straussman

Journal of fungi (Basel, Switzerland) 8: PubMed35448568

2022

Comparison Approach for Identifying Missed Invasive Fungal Infections in Formalin-Fixed, Paraffin-Embedded Autopsy Specimens.

Applications

Unspecified application

Species

Unspecified reactive species

Sota Sadamoto,Yurika Mitsui,Yasuhiro Nihonyanagi,Kazuki Amemiya,Minoru Shinozaki,Somay Yamagata Murayama,Masahiro Abe,Takashi Umeyama,Naobumi Tochigi,Yoshitsugu Miyazaki,Kazutoshi Shibuya

PloS one 16:e0252948 PubMed34242260

2021

Variation of virulence of five Aspergillus fumigatus isolates in four different infection models.

Applications

Unspecified application

Species

Unspecified reactive species

E M Keizer,I D Valdes,G Forn-Cuni,E Klijn,A H Meijer,F Hillman,H A B Wösten,H de Cock

European journal of immunology 50:1712-1728 PubMed32558930

2020

The domestic pig as human-relevant large animal model to study adaptive antifungal immune responses against airborne Aspergillus fumigatus.

Applications

Unspecified application

Species

Unspecified reactive species

Stefanie Schmidt,Friederike Ebner,Kerstin Rosen,Olaf Kniemeyer,Axel A Brakhage,Jürgen Löffler,Michelle Seif,Jan Springer,Josephine Schlosser,Lydia Scharek-Tedin,Alexander Scheffold,Petra Bacher,Anja A Kühl,Uwe Rösler,Susanne Hartmann

Medical mycology case reports 7:15-9 PubMed27330942

2015

Invasive aspergillosis in the aortic arch with infectious Aspergillus lesions in pulmonary bullae.

Applications

Unspecified application

Species

Unspecified reactive species

Isao Watanabe,Takahisa Nakayama,Eiji Yamada,Mitsuhiro Tsukino,Eiichi Hayashi

Veterinary pathology 50:343-6 PubMed22711744

2012

Probable Blastomyces dermatitidis infection in a young rat.

Applications

Unspecified application

Species

Unspecified reactive species

S C Chang,S L Hsuan,C C Lin,W C Lee,M S Chien,L C Chen,J H Wu,S J Cheng,C L Chen,J W Liao

PLoS pathogens 7:e1002145 PubMed21811407

2011

In vivo hypoxia and a fungal alcohol dehydrogenase influence the pathogenesis of invasive pulmonary aspergillosis.

Applications

IHC-Fr

Species

Unspecified reactive species

Nora Grahl,Srisombat Puttikamonkul,Jeffrey M Macdonald,Michael P Gamcsik,Lisa Y Ngo,Tobias M Hohl,Robert A Cramer
View all publications

Product promise

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