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AB238106

Anti-ASPM antibody

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(7 Publications)

Rabbit Polyclonal ASPM antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ASPM aa 100-250.

View Alternative Names

MCPH5, ASPM, Abnormal spindle-like microcephaly-associated protein, Abnormal spindle protein homolog, Asp homolog

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASPM antibody (AB238106)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASPM antibody (AB238106)

Paraffin-embedded human lung tissue stained for ASPM using ab238106 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASPM antibody (AB238106)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASPM antibody (AB238106)

Paraffin-embedded human adrenal gland tissue stained for ASPM using ab238106 at 1/100 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-ASPM antibody (AB238106)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ASPM antibody (AB238106)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ASPM (green) using ab238106 at 1/100 dilution in ICC/IF, followed by Alexa Fluor® 488-conjugated goat anti-rabbit IgG (H+L).

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human ASPM aa 100-250. The exact immunogen used to generate this antibody is proprietary information.

Q8IZT6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Colobus": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cynomolgus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Lar gibbon": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity greater than 95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ASPM also known by its alternative name "Abnormal Spindle Microtubule Assembly" is a protein that plays an important mechanical role in cell division. It is involved in mitotic spindle formation contributing to chromosome segregation during mitosis. The ASPM protein has a mass of approximately 410 kDa. This protein is expressed notably in neural tissues and regions of high cellular proliferation such as the brain and other rapidly dividing cell populations.
Biological function summary

ASPM supports proper neural development by maintaining the orientation of the mitotic spindle during neurogenesis. It does not function alone but as part of a protein complex involved in regulating mitotic spindle assembly. It associates with other mitotic spindle-associated proteins to ensure precise cell division. Through this it influences cell fate decisions and proliferation within developing tissues.

Pathways

ASPM plays a significant role in the cell cycle particularly during the mitosis phase. It is an essential part of the mitotic spindle checkpoint pathway which ensures accurate chromosome alignment and segregation. ASPM interacts with proteins like CDK1 (Cyclin-dependent kinase 1) to modulate these processes. This involvement secures ordered progression through cell division and prevents aneuploidy by ensuring spindle apparatuses function correctly.

ASPM dysfunction is closely linked to microcephaly where individuals have a significantly reduced brain size. Genetic mutations in the ASPM gene lead to some forms of primary microcephaly. The protein's influence on neurogenesis ties closely to this condition. Additionally ASPM has associations with cancer particularly in tumors where abnormal cell proliferation occurs. Alterations in ASPM expression can coincide with other cancer-related proteins such as TP53 revealing its potential role as a biomarker in tumor development and growth.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in mitotic spindle regulation and coordination of mitotic processes. The function in regulating microtubule dynamics at spindle poles including spindle orientation, astral microtubule density and poleward microtubule flux seems to depend on the association with the katanin complex formed by KATNA1 and KATNB1. Enhances the microtubule lattice severing activity of KATNA1 by recruiting the katanin complex to microtubules. Can block microtubule minus-end growth and reversely this function can be enhanced by the katanin complex (PubMed : 28436967). May have a preferential role in regulating neurogenesis.
See full target information ASPM
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Open life sciences 19:20220990 PubMed39759103

2025

MFAP5 inhibits the malignant progression of endometrial cancer cells .

Applications

Unspecified application

Species

Unspecified reactive species

Guanying Liang,Zijuan Qi,Chun Du

BMC cancer 24:1151 PubMed39289658

2024

Neuroblastoma with high ASPM reveals pronounced heterogeneity and poor prognosis.

Applications

Unspecified application

Species

Unspecified reactive species

Chao Li,Xueyuan Lu,Fengxian Zhang,Shuo Huang,Lin Ding,Hui Wang,Suyun Chen

Translational cancer research 10:983-997 PubMed35116426

2022

Overexpression of abnormal spindle-like microcephaly-associated (ASPM) increases tumor aggressiveness and predicts poor outcome in patients with lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenxing Feng,Jiao Zhang,Yafang Zheng,Jianchao Liu,Tianyu Duan,Tieshuan Tian

Journal of clinical laboratory analysis 35:e23931 PubMed34398984

2021

METTL3-mediated m6A methylation of ASPM drives hepatocellular carcinoma cells growth and metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

An Wang,Xiaofeng Chen,Dongen Li,Liang Yang,Jianshuai Jiang

Experimental and therapeutic medicine 22:1154 PubMed34504599

2021

ASPM combined with KIF11 promotes the malignant progression of hepatocellular carcinoma via the Wnt/β-catenin signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Wu,Chunyang Hu,Lianbao Kong

International journal of oncology 58: PubMed33786609

2021

Oncogenic role of abnormal spindle‑like microcephaly‑associated protein in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jiang Wang,Jinghui Liang,Haixia Li,Jingyi Han,Jin Jiang,Yongmeng Li,Zitong Feng,Renchang Zhao,Hui Tian

Zhongguo fei ai za zhi = Chinese journal of lung cancer 23:29-35 PubMed31948535

2020

[Expression of ASPM in Lung Adenocarcinoma and Its Relationship with Development and Prognosis].

Applications

Unspecified application

Species

Unspecified reactive species

Junjie Wang,Zhenyu He,Renhui Duan
View all publications
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