Rabbit Polyclonal ASPM antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ASPM aa 100-250.
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Publications
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- Translational cancer research 10:983-9972022Overexpression of abnormal spindle-like microcephaly-associated (ASPM) increases tumor aggressiveness and predicts poor outcome in patients with lung adenocarcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhenxing Feng,Jiao Zhang,Yafang Zheng,Jianchao Liu,Tianyu Duan,Tieshuan TianPubMed 35116426
- Journal of clinical laboratory analysis 35:e239312021METTL3-mediated m6A methylation of ASPM drives hepatocellular carcinoma cells growth and metastasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAn Wang,Xiaofeng Chen,Dongen Li,Liang Yang,Jianshuai JiangPubMed 34398984
- Experimental and therapeutic medicine 22:11542021ASPM combined with KIF11 promotes the malignant progression of hepatocellular carcinoma via the Wnt/β-catenin signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBin Wu,Chunyang Hu,Lianbao KongPubMed 34504599
- International journal of oncology 58:2021Oncogenic role of abnormal spindle‑like microcephaly‑associated protein in lung adenocarcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiang Wang,Jinghui Liang,Haixia Li,Jingyi Han,Jin Jiang,Yongmeng Li,Zitong Feng,Renchang Zhao,Hui TianPubMed 33786609
- Zhongguo fei ai za zhi = Chinese journal of lung cancer 23:29-352020[Expression of ASPM in Lung Adenocarcinoma and Its Relationship with Development and Prognosis].Applications:Unspecified applicationReactive species:Unspecified reactive speciesJunjie Wang,Zhenyu He,Renhui DuanPubMed 31948535
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human ASPM aa 100-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q8IZT6
Reactivity data
Select an application| IHC-P | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Chimpanzee | Predicted | Predicted |
| Colobus | Predicted | Predicted |
| Cynomolgus monkey | Predicted | Predicted |
| Gorilla | Predicted | Predicted |
| Lar gibbon | Predicted | Predicted |
| Orangutan | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20.00000 - 1/200.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Colobus, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan, Lar gibbon | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Colobus, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan, Lar gibbon | Dilution info - | Notes - |
Target data
Function
Involved in mitotic spindle regulation and coordination of mitotic processes. The function in regulating microtubule dynamics at spindle poles including spindle orientation, astral microtubule density and poleward microtubule flux seems to depend on the association with the katanin complex formed by KATNA1 and KATNB1. Enhances the microtubule lattice severing activity of KATNA1 by recruiting the katanin complex to microtubules. Can block microtubule minus-end growth and reversely this function can be enhanced by the katanin complex (PubMed:28436967). May have a preferential role in regulating neurogenesis.
Alternative names
MCPH5, ASPM, Abnormal spindle-like microcephaly-associated protein, Abnormal spindle protein homolog, Asp homolog
Recommended products
Rabbit Polyclonal ASPM antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ASPM aa 100-250.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human ASPM aa 100-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q8IZT6
- Purification technique
- Affinity purification Protein G
- Concentration
- Purification notes
Purity greater than 95%.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ASPM also known by its alternative name "Abnormal Spindle Microtubule Assembly" is a protein that plays an important mechanical role in cell division. It is involved in mitotic spindle formation contributing to chromosome segregation during mitosis. The ASPM protein has a mass of approximately 410 kDa. This protein is expressed notably in neural tissues and regions of high cellular proliferation such as the brain and other rapidly dividing cell populations.
- Biological function summary
ASPM supports proper neural development by maintaining the orientation of the mitotic spindle during neurogenesis. It does not function alone but as part of a protein complex involved in regulating mitotic spindle assembly. It associates with other mitotic spindle-associated proteins to ensure precise cell division. Through this it influences cell fate decisions and proliferation within developing tissues.
- Pathways
ASPM plays a significant role in the cell cycle particularly during the mitosis phase. It is an essential part of the mitotic spindle checkpoint pathway which ensures accurate chromosome alignment and segregation. ASPM interacts with proteins like CDK1 (Cyclin-dependent kinase 1) to modulate these processes. This involvement secures ordered progression through cell division and prevents aneuploidy by ensuring spindle apparatuses function correctly.
- Associated diseases and disorders
ASPM dysfunction is closely linked to microcephaly where individuals have a significantly reduced brain size. Genetic mutations in the ASPM gene lead to some forms of primary microcephaly. The protein's influence on neurogenesis ties closely to this condition. Additionally ASPM has associations with cancer particularly in tumors where abnormal cell proliferation occurs. Alterations in ASPM expression can coincide with other cancer-related proteins such as TP53 revealing its potential role as a biomarker in tumor development and growth.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASPM antibody (ab238106)
Paraffin-embedded human adrenal gland tissue stained for ASPM using ab238106 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASPM antibody (ab238106)
Paraffin-embedded human lung tissue stained for ASPM using ab238106 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence - Anti-ASPM antibody (ab238106)
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ASPM (green) using ab238106 at 1/100 dilution in ICC/IF, followed by Alexa Fluor® 488-conjugated goat anti-rabbit IgG (H+L).
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