AB154404

Anti-Asporin antibody

Name: Anti-Asporin antibody (ab154404) | Abcam
Brand: Abcam Limited
SKU: AB154404
Price: 230 USD
Availability: InStock
Rating: 1 (1 reviews)

(1 Review)

(4 Publications)

Rabbit Polyclonal Asporin antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human ASPN aa 100-250.

View Alternative Names

PLAP1, SLRR1C, UNQ215/PRO241, ASPN, Asporin, Periodontal ligament-associated protein 1, PLAP-1

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-Asporin antibody (AB154404)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Asporin antibody (AB154404)

Immunofluorescence analysis of methanol-fixed HeLa cells, labeling Asporin using ab154404 at 1/200 dilution. Right panel costained with Hoechst 33342.

Supplier Data

Western blot - Anti-Asporin antibody (AB154404)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-Asporin antibody (ab154404) at 1/1000 dilution

All lanes:

H1299 Whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 43 kDa

false

Supplier Data

Western blot - Anti-Asporin antibody (AB154404)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-Asporin antibody (ab154404) at 1/500 dilution

All lanes:

Rat heart tissue extract at 50 µg

Predicted band size: 43 kDa

false

Supplier Data

Western blot - Anti-Asporin antibody (AB154404)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-Asporin antibody (ab154404) at 1/500 dilution

All lanes:

Mouse liver tissue extract at 50 µg

Predicted band size: 43 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Mouse, Rat

Applications

ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human ASPN aa 100-250. The exact immunogen used to generate this antibody is proprietary information.

Q9BXN1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 10% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Asporin also known as ASPN is a small leucine-rich proteoglycan. Asporin weighs about 39 kDa. It expresses mostly in cartilage with notable presence in fibrocartilage and intervertebral discs. Scientists have identified asporin as a regulator of extracellular matrix organization and a mediator in cellular processes such as cell adhesion and migration. In Singapore and other places research on this protein has gained significant attention due to its roles in tissue development and potential clinical implications.

Biological function summary

The protein functions in modulating the extracellular matrix and influences mineralization of tissues. It belongs to a family of small leucine-rich repeat proteins that typically form part of larger complexes. Asporin interacts with TGF-β (transforming growth factor-beta) inhibiting its signaling pathway. This interaction affects collagen fibrillogenesis and may influence tissue repair processes. The ability of asporin to bind calcium hints at its role in calcification processes as well.

Pathways

Asporin plays a significant role in the regulation of the TGF-β and BMP (bone morphogenetic protein) signaling pathways. These pathways are key in controlling cell growth and differentiation particularly in connective tissues. Through these pathways asporin interacts with proteins like collagen I and II as well as other matrix components. It’s involved in fine-tuning processes such as skeletal development and cartilage homeostasis.

Research reveals connections between asporin and osteoarthritis as well as intervertebral disc disease. Variants in asporin known as polymorphisms have been linked to susceptibility to osteoarthritis in certain populations. Its interaction with TGF-β impacts disease progression implicating other proteins such as matrix metalloproteinases in the extracellular matrix breakdown. Asporin's modulation of tissue mineralization also relates to intervertebral disc degeneration providing insights into potential therapeutic targets.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Negatively regulates periodontal ligament (PDL) differentiation and mineralization to ensure that the PDL is not ossified and to maintain homeostasis of the tooth-supporting system. Inhibits BMP2-induced cytodifferentiation of PDL cells by preventing its binding to BMPR1B/BMP type-1B receptor, resulting in inhibition of BMP-dependent activation of SMAD proteins (By similarity). Critical regulator of TGF-beta in articular cartilage and plays an essential role in cartilage homeostasis and osteoarthritis (OA) pathogenesis. Negatively regulates chondrogenesis in the articular cartilage by blocking the TGF-beta/receptor interaction on the cell surface and inhibiting the canonical TGF-beta/Smad signal. Binds calcium and plays a role in osteoblast-driven collagen biomineralization activity.

See full target information ASPN

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Experimental dermatology 33:e15186 PubMed39367569

2024

Inhibition of Ubiquitin C-Terminal Hydrolase L1 Facilitates Cutaneous Wound Healing via Activating TGF-β/Smad Signalling Pathway in Fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Huihui Pan,Jinru Song,Qing An,Junyi Chen,Wenyue Zheng,Litian Zhang,Jingjing Gu,Chengcheng Deng,Bin Yang

Frontiers in molecular biosciences 9:805235 PubMed35300115

2022

Integrated Strategies of Diverse Feature Selection Methods Identify Aging-Based Reliable Gene Signatures for Ischemic Cardiomyopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Huafeng Song,Shaoze Chen,Tingting Zhang,Xiaofei Huang,Qiyu Zhang,Cuizhi Li,Chunlin Chen,Shaoxian Chen,Dehui Liu,Jiawen Wang,Yingfeng Tu,Yueheng Wu,Youbin Liu

International journal of cell biology 2020:2045969 PubMed32328102

2020

Asporin Reduces Adult Aortic Valve Interstitial Cell Mineralization Induced by Osteogenic Media and Wnt Signaling Manipulation .

Applications

Unspecified application

Species

Unspecified reactive species

Anisha Polley,Riffat Khanam,Arunima Sengupta,Santanu Chakraborty

Proteomics 15:2934-44 PubMed25914152

2015

Unravelling the proteome of degenerative human mitral valves.

Applications

Species

Human

Hwee Tong Tan,Teck Kwang Lim,Arthur Mark Richards,Theodoros Kofidis,Kristine Leok-Kheng Teoh,Lieng H Ling,Maxey C M Chung

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Asporin antibody

Immunocytochemistry/ Immunofluorescence - Anti-Asporin antibody (AB154404)

Western blot - Anti-Asporin antibody (AB154404)

Western blot - Anti-Asporin antibody (AB154404)

Western blot - Anti-Asporin antibody (AB154404)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Complementary Products

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Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise