Rabbit Polyclonal Asporin antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human ASPN aa 100-250.
Images
Publications
Filter by
- Frontiers in molecular biosciences 9:8052352022Integrated Strategies of Diverse Feature Selection Methods Identify Aging-Based Reliable Gene Signatures for Ischemic Cardiomyopathy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuafeng Song,Shaoze Chen,Tingting Zhang,Xiaofei Huang,Qiyu Zhang,Cuizhi Li,Chunlin Chen,Shaoxian Chen,Dehui Liu,Jiawen Wang,Yingfeng Tu,Yueheng Wu,Youbin LiuPubMed 35300115
- International journal of cell biology 2020:20459692020Asporin Reduces Adult Aortic Valve Interstitial Cell Mineralization Induced by Osteogenic Media and Wnt Signaling Manipulation .Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnisha Polley et. al.PubMed 32328102
- Proteomics 15:2934-442015Unravelling the proteome of degenerative human mitral valves.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 89.99% PBS, 10% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human ASPN aa 100-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BXN1
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
Target data
Function
Negatively regulates periodontal ligament (PDL) differentiation and mineralization to ensure that the PDL is not ossified and to maintain homeostasis of the tooth-supporting system. Inhibits BMP2-induced cytodifferentiation of PDL cells by preventing its binding to BMPR1B/BMP type-1B receptor, resulting in inhibition of BMP-dependent activation of SMAD proteins (By similarity). Critical regulator of TGF-beta in articular cartilage and plays an essential role in cartilage homeostasis and osteoarthritis (OA) pathogenesis. Negatively regulates chondrogenesis in the articular cartilage by blocking the TGF-beta/receptor interaction on the cell surface and inhibiting the canonical TGF-beta/Smad signal. Binds calcium and plays a role in osteoblast-driven collagen biomineralization activity.
Alternative names
PLAP1, SLRR1C, UNQ215/PRO241, ASPN, Asporin, Periodontal ligament-associated protein 1, PLAP-1
Recommended products
Rabbit Polyclonal Asporin antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human ASPN aa 100-250.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 89.99% PBS, 10% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human ASPN aa 100-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BXN1
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Asporin also known as ASPN is a small leucine-rich proteoglycan. Asporin weighs about 39 kDa. It expresses mostly in cartilage with notable presence in fibrocartilage and intervertebral discs. Scientists have identified asporin as a regulator of extracellular matrix organization and a mediator in cellular processes such as cell adhesion and migration. In Singapore and other places research on this protein has gained significant attention due to its roles in tissue development and potential clinical implications.
- Biological function summary
The protein functions in modulating the extracellular matrix and influences mineralization of tissues. It belongs to a family of small leucine-rich repeat proteins that typically form part of larger complexes. Asporin interacts with TGF-β (transforming growth factor-beta) inhibiting its signaling pathway. This interaction affects collagen fibrillogenesis and may influence tissue repair processes. The ability of asporin to bind calcium hints at its role in calcification processes as well.
- Pathways
Asporin plays a significant role in the regulation of the TGF-β and BMP (bone morphogenetic protein) signaling pathways. These pathways are key in controlling cell growth and differentiation particularly in connective tissues. Through these pathways asporin interacts with proteins like collagen I and II as well as other matrix components. It’s involved in fine-tuning processes such as skeletal development and cartilage homeostasis.
- Associated diseases and disorders
Research reveals connections between asporin and osteoarthritis as well as intervertebral disc disease. Variants in asporin known as polymorphisms have been linked to susceptibility to osteoarthritis in certain populations. Its interaction with TGF-β impacts disease progression implicating other proteins such as matrix metalloproteinases in the extracellular matrix breakdown. Asporin's modulation of tissue mineralization also relates to intervertebral disc degeneration providing insights into potential therapeutic targets.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Western blot - Anti-Asporin antibody (ab154404)
All lanes: Western blot - Anti-Asporin antibody (ab154404) at 1/1000 dilution
Lane 1: A549 whole cell lysate/extract at 30 µg
Lane 2: H1299 whole cell lysate/extract at 30 µg
Lane 3: HCT116 whole cell lysate/extract at 30 µg
Predicted band size: 43 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Asporin antibody (ab154404)
Immunofluorescence analysis of methanol-fixed HeLa cells, labeling Asporin using ab154404 at 1/200 dilution. Right panel costained with Hoechst 33342.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com