AB31303

Anti-Asporin antibody

Name: Anti-Asporin antibody (ab31303) | Abcam
Brand: Abcam Limited
SKU: AB31303
Price: 530 USD
Availability: InStock
Rating: 4 (3 reviews)

(3 Reviews)

(12 Publications)

Goat Polyclonal Asporin antibody. Suitable for ICC and reacts with Human samples. Cited in 12 publications. Immunogen corresponding to Synthetic Peptide within Human ASPN aa 150-200.

View Alternative Names

PLAP1, SLRR1C, UNQ215/PRO241, ASPN, Asporin, Periodontal ligament-associated protein 1, PLAP-1

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Supplier Data

Immunocytochemistry - Anti-Asporin antibody (AB31303)

Immunocytochemistry analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Incubated with ab31303 for 1 hour (10μg/ml) followed by Alexa Fluor^® 488 secondary antibody (4μg/ml), showing nuclear membrane staining. The nuclear stain is DAPI (blue).

Negative control : Unimmunized goat IgG (10μg/ml) followed by Alexa Fluor^® 488 secondary antibody (4μg/ml).

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC

applications

Immunogen

Synthetic Peptide within Human ASPN aa 150-200. The exact immunogen used to generate this antibody is proprietary information.

Q9BXN1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "<p></p>" }, "Mouse": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "" }, "Rat": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Asporin also known as ASPN is a small leucine-rich proteoglycan. Asporin weighs about 39 kDa. It expresses mostly in cartilage with notable presence in fibrocartilage and intervertebral discs. Scientists have identified asporin as a regulator of extracellular matrix organization and a mediator in cellular processes such as cell adhesion and migration. In Singapore and other places research on this protein has gained significant attention due to its roles in tissue development and potential clinical implications.

Biological function summary

The protein functions in modulating the extracellular matrix and influences mineralization of tissues. It belongs to a family of small leucine-rich repeat proteins that typically form part of larger complexes. Asporin interacts with TGF-β (transforming growth factor-beta) inhibiting its signaling pathway. This interaction affects collagen fibrillogenesis and may influence tissue repair processes. The ability of asporin to bind calcium hints at its role in calcification processes as well.

Pathways

Asporin plays a significant role in the regulation of the TGF-β and BMP (bone morphogenetic protein) signaling pathways. These pathways are key in controlling cell growth and differentiation particularly in connective tissues. Through these pathways asporin interacts with proteins like collagen I and II as well as other matrix components. It’s involved in fine-tuning processes such as skeletal development and cartilage homeostasis.

Research reveals connections between asporin and osteoarthritis as well as intervertebral disc disease. Variants in asporin known as polymorphisms have been linked to susceptibility to osteoarthritis in certain populations. Its interaction with TGF-β impacts disease progression implicating other proteins such as matrix metalloproteinases in the extracellular matrix breakdown. Asporin's modulation of tissue mineralization also relates to intervertebral disc degeneration providing insights into potential therapeutic targets.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Negatively regulates periodontal ligament (PDL) differentiation and mineralization to ensure that the PDL is not ossified and to maintain homeostasis of the tooth-supporting system. Inhibits BMP2-induced cytodifferentiation of PDL cells by preventing its binding to BMPR1B/BMP type-1B receptor, resulting in inhibition of BMP-dependent activation of SMAD proteins (By similarity). Critical regulator of TGF-beta in articular cartilage and plays an essential role in cartilage homeostasis and osteoarthritis (OA) pathogenesis. Negatively regulates chondrogenesis in the articular cartilage by blocking the TGF-beta/receptor interaction on the cell surface and inhibiting the canonical TGF-beta/Smad signal. Binds calcium and plays a role in osteoblast-driven collagen biomineralization activity.

See full target information ASPN

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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Molecules and cells 47:100059 PubMed38554844

2024

Single-cell transcriptome analysis reveals periodontal ligament fibroblast heterogeneity with distinct IL-1β and RANKL expression in periodontitis.

Applications

Unspecified application

Species

Unspecified reactive species

Shenzheng Mo,Ji Sun Jang,Seung Hye Lee,Hong-Hee Kim

iScience 26:107200 PubMed37554462

2023

Temporal transcriptome features identify early skeletal commitment during human epiphysis development at single-cell resolution.

Applications

Unspecified application

Species

Unspecified reactive species

Zhonghao Deng,Shengwei Rong,Lu Gan,Fuhua Wang,Liangxiao Bao,Fang Cai,Zheting Liao,Yu Jin,Shuhao Feng,Zihang Feng,Yiran Wei,Ruge Chen,Yangchen Jin,Yanli Zhou,Xiaoyong Zheng,Liping Huang,Liang Zhao

Orthopaedic surgery 15:2423-2434 PubMed37427673

2023

ASPN Synergizes with HAPLN1 to Inhibit the Osteogenic Differentiation of Bone Marrow Mesenchymal Stromal Cells and Extracellular Matrix Mineralization of Osteoblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Guohui Zhou,Xinmin Yan,Zhenfei Chen,Xing Zeng,Fangqian Wu

Journal of gastrointestinal oncology 13:2234-2248 PubMed36388685

2022

Identification of a five m6A-relevant mRNAs signature and risk score for the prognostication of gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Yin,Shichun Feng,Yongqiang Sun,Yasu Jiang,Chong Tang,Dongwei Sun

Matrix biology : journal of the International Society for Matrix Biology 110:40-59 PubMed35470068

2022

Asporin, an extracellular matrix protein, is a beneficial regulator of cardiac remodeling.

Applications

Unspecified application

Species

Unspecified reactive species

Chengqun Huang,Ankush Sharma,Reetu Thakur,Deepika Rai,Madhusudhanarao Katiki,Juliana de Freitas Germano,Yang Song,Sakshi Singh,Jon Sin,David Sengstock,Allen M Andres,Ramachandran Murali,Robert M Mentzer,Roberta A Gottlieb,Honit Piplani

Theranostics 12:1074-1096 PubMed35154475

2022

Single-cell RNA landscape of the osteoimmunology microenvironment in periodontitis.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Chen,Hua Wang,Qiudong Yang,Wenhua Zhao,Yuyi Chen,Qiaoqi Ni,Wenlei Li,Jiali Shi,Wei Zhang,Lu Li,Yan Xu,Hengwei Zhang,Dengshun Miao,Lianping Xing,Wen Sun

Life sciences 256:117955 PubMed32534038

2020

Low SCRIB expression in fibroblasts promotes invasion of lung cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Wang,Haiyang Liu,Yangyang Bian,Junfeng An,Xixi Duan,Jiajia Wan,Xiaohan Yao,Cancan Du,Chen Ni,Linyu Zhu,Ningjing Lei,Fei Wang,Lijing Zhang,Ming Wang,Zhihai Qin

Nature communications 11:2604 PubMed32451392

2020

Activin-mediated alterations of the fibroblast transcriptome and matrisome control the biomechanical properties of skin wounds.

Applications

Unspecified application

Species

Unspecified reactive species

Mateusz S Wietecha,Marco Pensalfini,Michael Cangkrama,Bettina Müller,Juyoung Jin,Jürgen Brinckmann,Edoardo Mazza,Sabine Werner

PloS one 12:e0184028 PubMed28859141

2017

Asporin-deficient mice have tougher skin and altered skin glycosaminoglycan content and structure.

Applications

Unspecified application

Species

Unspecified reactive species

Marco Maccarana,René B Svensson,Anki Knutsson,Antonis Giannopoulos,Mea Pelkonen,MaryAnn Weis,David Eyre,Matthew Warman,Sebastian Kalamajski

Biomaterials 32:7106-17 PubMed21774982

2011

Discontinuities in the human bone-PDL-cementum complex.

Applications

ICC/IF

Species

Human

Jonathan M Hurng,Michael P Kurylo,Grayson W Marshall,Samuel M Webb,Mark I Ryder,Sunita P Ho

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Asporin antibody

Immunocytochemistry - Anti-Asporin antibody (AB31303)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (12)

Product promise