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AB124465

Anti-ASS1 antibody [2B10]

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(13 Publications)

Anti-ASS1 antibody [2B10] (ab124465) is a mouse monoclonal antibody detecting ASS1 in Western Blot, Flow Cytometry, ICC/IF, ELISA. Suitable for African green monkey, Human, Mouse, Rat.

- KO validated for confirmed specificity
- Over 10 publications

View Alternative Names

ASS, ASS1, Argininosuccinate synthase, Citrulline--aspartate ligase

7 Images
Immunocytochemistry/ Immunofluorescence - Anti-ASS1 antibody [2B10] (AB124465)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ASS1 antibody [2B10] (AB124465)

ab124465 staining ASS1 in wild-type HeLa cells (top panel) and ASS1 knockout HeLa cells (ab264989) (bottom panel). The cells were fixed with 100% methanol (5 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab124465 at 1/1000 dilution and ab6046 (Rabbit polyclonal to beta Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to mouse IgG (Alexa Fluor® 488) (ab150117) at 2 μg/ml (shown in green) and a goat secondary antibody to rabbit IgG (Alexa Fluor® 594) (ab150080) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).

Flow Cytometry - Anti-ASS1 antibody [2B10] (AB124465)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-ASS1 antibody [2B10] (AB124465)

ab124465, at 1/200 dilution, staining ASS1 in Jurkat cells (green) by flow cytometry or negative control (red).

Immunocytochemistry/ Immunofluorescence - Anti-ASS1 antibody [2B10] (AB124465)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ASS1 antibody [2B10] (AB124465)

ab124465, at 1/200 dilution, staining ASS1 in HeLa cells by Immunofluorescence (green). Blue : DRAQ5 fluorescent DNA dye.

Western blot - Anti-ASS1 antibody [2B10] (AB124465)
  • WB

Supplier Data

Western blot - Anti-ASS1 antibody [2B10] (AB124465)

Lane 1 : Wild type HAP1 whole cell lysate (20 μg)
Lane 2 : ASS1 knockout HAP1 whole cell lysate (20 μg)
Lane 3 : HeLa whole cell lysate (20 μg)
Lane 4 : Jurkat whole cell lysate (20 μg)

Lanes 1 - 4 : Merged signal (red and green). Green - ab124465 observed at 47 kDa. Red - loading control, ab181602, observed at 37 kDa.

ab124465 was shown to recognize ASS1 when ASS1 knockout samples were used, along with additional cross-reactive bands. Wild-type and ASS1 knockout samples were subjected to SDS-PAGE. ab124465 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at a 1/500 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-ASS1 antibody [2B10] (ab124465)

Predicted band size: 47 kDa

false

ELISA - Anti-ASS1 antibody [2B10] (AB124465)
  • ELISA

Unknown

ELISA - Anti-ASS1 antibody [2B10] (AB124465)

ELISA using ab124465.

Western blot - Anti-ASS1 antibody [2B10] (AB124465)
  • WB

Unknown

Western blot - Anti-ASS1 antibody [2B10] (AB124465)

All lanes:

Western blot - Anti-ASS1 antibody [2B10] (ab124465) at 1/500 dilution

Lane 1:

A431 cell lysate

Lane 2:

Raji cell lysate

Lane 3:

L1210 cell lysate

Lane 4:

MOLT4 cell lysate

Lane 5:

Jurkat cell lysate

Lane 6:

A549 cell lysate

Lane 7:

NIH 3T3 cell lysate

Lane 8:

PC12 cell lysate

Lane 9:

COS7 cell lysate

Predicted band size: 47 kDa

false

Western blot - Anti-ASS1 antibody [2B10] (AB124465)
  • WB

Unknown

Western blot - Anti-ASS1 antibody [2B10] (AB124465)

All lanes:

Western blot - Anti-ASS1 antibody [2B10] (ab124465) at 1/500 dilution

All lanes:

Human ASS1 recombinant protein (aa: 40-236)

Predicted band size: 19 kDa,47 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

2B10

Isotype

IgG1

Carrier free

No

Reacts with

Mouse, Rat, Human, African green monkey

Applications

ELISA, Flow Cyt, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human ASS1. The exact immunogen used to generate this antibody is proprietary information.

P00966

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Reactivity data

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Product details

What is this antibody validated in?
Anti-ASS1 antibody [2B10] (ab124465) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunocytochemistry/immunofluorescence (ICC/IF), ELISA in African green monkey, Human, Mouse, Rat samples.

What is the molecular weight of ASS1?
Anti-ASS1 [2B10] (ab124465) specifically detects a band for ASS1 (UniProt: P00966) at a molecular weight of 47kDa.

Trusted by the scientific community
Anti-ASS1 [2B10] (ab124465) was first used in a scientific publication in 2012 and has been cited over 10 times in peer-reviewed journals.

Specificity confirmed
The specificity of Anti-ASS1 antibody [2B10] (ab124465) has been confirmed by Western blot testing in ASS1 Knockout HAP1 cell line, ab264989.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purified from tissue culture supernatant.
Storage buffer
Preservative: 0.05% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ASS1 or argininosuccinate synthase 1 is an enzyme that plays an important role in the urea cycle. It catalyzes the conversion of citrulline and aspartate into argininosuccinate using ATP in the process. The ASS1 protein is commonly expressed in the liver where it actively participates in the detoxification of ammonia. It has a molecular mass of approximately 46 kDa. Some alternate names for this protein include ASS and ASS-1.
Biological function summary

Argininosuccinate synthase 1 contributes significantly to the synthesis of arginine an essential amino acid through its conversion activities. The enzyme operates as a homotrimer a complex consisting of three identical subunits. This enzymatic activity is indispensable in maintaining the balance of nitrogen and ammonia within the body particularly important for liver and kidney functions.

Pathways

ASS1 is a critical component of both the urea cycle and the nitric oxide metabolism pathway. The urea cycle helps in detoxifying ammonia by converting it into urea which is excreted in urine. In nitric oxide metabolism ASS1 links with nitric oxide synthase to facilitate the use of arginine in producing nitric oxide a vital signaling molecule. The pathway engagement partners include proteins like citrin which supplies aspartate for the reaction.

ASS1 has implications in citrullinemia and hepatocellular carcinoma. Citrullinemia results from mutations in the ASS1 gene leading to defective urea cycle function and ammonia accumulation. In cancer particularly hepatocellular carcinoma low expression of ASS1 correlates with poor prognosis as cancer cells often exhibit arginine auxotrophy. Connections through these conditions involve interactions with enzymes such as arginase which also influences arginine availability.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

One of the enzymes of the urea cycle, the metabolic pathway transforming neurotoxic amonia produced by protein catabolism into inocuous urea in the liver of ureotelic animals. Catalyzes the formation of arginosuccinate from aspartate, citrulline and ATP and together with ASL it is responsible for the biosynthesis of arginine in most body tissues.
See full target information ASS1
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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Biomolecules 13: PubMed37189460

2023

Transcriptome Analysis of Redox Systems and Polyamine Metabolic Pathway in Hepatoma and Non-Tumor Hepatocyte-like Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Olga N Ivanova,George S Krasnov,Anastasiya V Snezhkina,Anna V Kudryavtseva,Vyacheslav S Fedorov,Natalia F Zakirova,Michail V Golikov,Sergey N Kochetkov,Birke Bartosch,Vladimir T Valuev-Elliston,Alexander V Ivanov

Nature communications 13:7830 PubMed36539415

2022

Dynamic partitioning of branched-chain amino acids-derived nitrogen supports renal cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Marco Sciacovelli,Aurelien Dugourd,Lorea Valcarcel Jimenez,Ming Yang,Efterpi Nikitopoulou,Ana S H Costa,Laura Tronci,Veronica Caraffini,Paulo Rodrigues,Christina Schmidt,Dylan Gerard Ryan,Timothy Young,Vincent R Zecchini,Sabrina H Rossi,Charlie Massie,Caroline Lohoff,Maria Masid,Vassily Hatzimanikatis,Christoph Kuppe,Alex Von Kriegsheim,Rafael Kramann,Vincent Gnanapragasam,Anne Y Warren,Grant D Stewart,Ayelet Erez,Sakari Vanharanta,Julio Saez-Rodriguez,Christian Frezza

Cell metabolism 34:1151-1167.e7 PubMed35839757

2022

GCN2 inhibition sensitizes arginine-deprived hepatocellular carcinoma cells to senolytic treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Rindert Missiaen,Nicole M Anderson,Laura C Kim,Bailey Nance,Michelle Burrows,Nicolas Skuli,Madeleine Carens,Romain Riscal,An Steensels,Fuming Li,M Celeste Simon

Cells 11: PubMed35406678

2022

Novel Blood Vascular Endothelial Subtype-Specific Markers in Human Skin Unearthed by Single-Cell Transcriptomic Profiling.

Applications

Unspecified application

Species

Unspecified reactive species

Yuliang He,Carlotta Tacconi,Lothar C Dieterich,Jihye Kim,Gaetana Restivo,Epameinondas Gousopoulos,Nicole Lindenblatt,Mitchell P Levesque,Manfred Claassen,Michael Detmar

Cancer & metabolism 9:40 PubMed34861885

2021

ASS1 and ASL suppress growth in clear cell renal cell carcinoma via altered nitrogen metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Sanika Khare,Laura C Kim,Graham Lobel,Paschalis-Thomas Doulias,Harry Ischiropoulos,Itzhak Nissim,Brian Keith,M Celeste Simon

Journal of experimental & clinical cancer research : CR 40:127 PubMed33838671

2021

Argininosuccinate synthase 1 suppresses tumor progression through activation of PERK/eIF2α/ATF4/CHOP axis in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Sanghwa Kim,Minji Lee,Yeonhwa Song,Su-Yeon Lee,Inhee Choi,I-Seul Park,Jiho Kim,Jin-Sun Kim,Kang Mo Kim,Haeng Ran Seo

International journal of molecular sciences 22: PubMed33806191

2021

Loss of Fer Jeopardizes Metabolic Plasticity and Mitochondrial Homeostasis in Lung and Breast Carcinoma Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Linoy Mehazri,Sally Shpungin,Shai Bel,Uri Nir

EMBO molecular medicine 13:e13158 PubMed33369168

2020

Beclin-1-mediated activation of autophagy improves proximal and distal urea cycle disorders.

Applications

Unspecified application

Species

Unspecified reactive species

Leandro R Soria,Sonam Gurung,Giulia De Sabbata,Dany P Perocheau,Angela De Angelis,Gemma Bruno,Elena Polishchuk,Debora Paris,Paola Cuomo,Andrea Motta,Michael Orford,Youssef Khalil,Simon Eaton,Philippa B Mills,Simon N Waddington,Carmine Settembre,Andrés F Muro,Julien Baruteau,Nicola Brunetti-Pierri

Journal of virology 93: PubMed30463977

2019

Oncogenic Kaposi's Sarcoma-Associated Herpesvirus Upregulates Argininosuccinate Synthase 1, a Rate-Limiting Enzyme of the Citrulline-Nitric Oxide Cycle, To Activate the STAT3 Pathway and Promote Growth Transformation.

Applications

Unspecified application

Species

Unspecified reactive species

Tingting Li,Ying Zhu,Fan Cheng,Chun Lu,Jae U Jung,Shou-Jiang Gao

Cancer research 79:518-533 PubMed30573518

2018

Acid-Induced Downregulation of ASS1 Contributes to the Maintenance of Intracellular pH in Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Alon Silberman,Omer Goldman,Odeya Boukobza Assayag,Adi Jacob,Shiran Rabinovich,Lital Adler,Joo Sang Lee,Rom Keshet,Alona Sarver,Julia Frug,Noa Stettner,Sivan Galai,Erez Persi,Keren Bahar Halpern,Yehudit Zaltsman-Amir,Ben Pode-Shakked,Raya Eilam,Yair Anikster,Sandesh C S Nagamani,Igor Ulitsky,Eytan Ruppin,Ayelet Erez
View all publications
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