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Mouse Monoclonal ASS1 antibody. Suitable for Flow Cyt, ELISA, WB, ICC/IF and reacts with Human, Mouse, Rat, African green monkey, Synthetic peptide, Recombinant fragment - Human samples. Cited in 13 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ASS1.

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Images

Flow Cytometry - Anti-ASS1 antibody [2B10] (AB124465), expandable thumbnail
  • Western blot - Anti-ASS1 antibody [2B10] (AB124465), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-ASS1 antibody [2B10] (AB124465), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-ASS1 antibody [2B10] (AB124465), expandable thumbnail
  • ELISA - Anti-ASS1 antibody [2B10] (AB124465), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

Preservative: 0.05% Sodium azide
Constituents: 99% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Fragment Protein within Human ASS1. The exact immunogen used to generate this antibody is proprietary information. Database link P00966

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytELISAWBICC/IF
Human
Tested
Expected
Tested
Tested
Mouse
Expected
Expected
Tested
Expected
Rat
Expected
Expected
Tested
Expected
African green monkey
Expected
Expected
Tested
Expected
Recombinant fragment - Human
Not recommended
Not recommended
Tested
Not recommended
Synthetic peptide
Not recommended
Tested
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/200.00000 - 1/400.00000
Notes

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Mouse, Rat, African green monkey
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide, Recombinant fragment - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Synthetic peptide
Dilution info
1/10000
Notes

-

Expected
Expected

Species
Human, Mouse, Rat, African green monkey
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Recombinant fragment - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/500.00000 - 1/2000.00000
Notes

-

Species
Rat
Dilution info
1/500.00000 - 1/2000.00000
Notes

-

Species
African green monkey
Dilution info
1/500.00000 - 1/2000.00000
Notes

-

Species
Recombinant fragment - Human
Dilution info
1/500.00000 - 1/2000.00000
Notes

-

Species
Human
Dilution info
1/500.00000 - 1/2000.00000
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/200.00000 - 1/1000.00000
Notes

-

Expected
Expected

Species
Mouse, Rat, African green monkey
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide, Recombinant fragment - Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

6 products for Alternative Product

Target data

Function

One of the enzymes of the urea cycle, the metabolic pathway transforming neurotoxic amonia produced by protein catabolism into inocuous urea in the liver of ureotelic animals. Catalyzes the formation of arginosuccinate from aspartate, citrulline and ATP and together with ASL it is responsible for the biosynthesis of arginine in most body tissues.

Alternative names

ASS, ASS1, Argininosuccinate synthase, Citrulline--aspartate ligase

Recommended products

Mouse Monoclonal ASS1 antibody. Suitable for Flow Cyt, ELISA, WB, ICC/IF and reacts with Human, Mouse, Rat, African green monkey, Synthetic peptide, Recombinant fragment - Human samples. Cited in 13 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ASS1.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Fragment Protein within Human ASS1. The exact immunogen used to generate this antibody is proprietary information. Database link P00966
Clone number
2B10
Purification technique
Affinity purification Protein G
Concentration
Loading...
Purification notes

Purified from tissue culture supernatant.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C

Notes

This product was changed from ascites to supernatant. Lot no's high than GR119162-30 are from Tissue Culture Supernatant

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

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Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

ASS1 or argininosuccinate synthase 1 is an enzyme that plays an important role in the urea cycle. It catalyzes the conversion of citrulline and aspartate into argininosuccinate using ATP in the process. The ASS1 protein is commonly expressed in the liver where it actively participates in the detoxification of ammonia. It has a molecular mass of approximately 46 kDa. Some alternate names for this protein include ASS and ASS-1.

Biological function summary

Argininosuccinate synthase 1 contributes significantly to the synthesis of arginine an essential amino acid through its conversion activities. The enzyme operates as a homotrimer a complex consisting of three identical subunits. This enzymatic activity is indispensable in maintaining the balance of nitrogen and ammonia within the body particularly important for liver and kidney functions.

Pathways

ASS1 is a critical component of both the urea cycle and the nitric oxide metabolism pathway. The urea cycle helps in detoxifying ammonia by converting it into urea which is excreted in urine. In nitric oxide metabolism ASS1 links with nitric oxide synthase to facilitate the use of arginine in producing nitric oxide a vital signaling molecule. The pathway engagement partners include proteins like citrin which supplies aspartate for the reaction.

Associated diseases and disorders

ASS1 has implications in citrullinemia and hepatocellular carcinoma. Citrullinemia results from mutations in the ASS1 gene leading to defective urea cycle function and ammonia accumulation. In cancer particularly hepatocellular carcinoma low expression of ASS1 correlates with poor prognosis as cancer cells often exhibit arginine auxotrophy. Connections through these conditions involve interactions with enzymes such as arginase which also influences arginine availability.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Flow Cytometry - Anti-ASS1 antibody [2B10] (ab124465), expandable thumbnail

    Flow Cytometry - Anti-ASS1 antibody [2B10] (ab124465)

    ab124465, at 1/200 dilution, staining ASS1 in Jurkat cells (green) by flow cytometry or negative control (red).

  • Western blot - Anti-ASS1 antibody [2B10] (ab124465), expandable thumbnail

    Western blot - Anti-ASS1 antibody [2B10] (ab124465)

    Lane 1: Wild type HAP1 whole cell lysate (20 μg)
    Lane 2: ASS1 knockout HAP1 whole cell lysate (20 μg)
    Lane 3: HeLa whole cell lysate (20 μg)
    Lane 4: Jurkat whole cell lysate (20 μg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab124465 observed at 47 kDa. Red - loading control, Anti-GAPDH antibody [EPR16891] - Loading Control ab181602, observed at 37 kDa.

    ab124465 was shown to recognize ASS1 when ASS1 knockout samples were used, along with additional cross-reactive bands. Wild-type and ASS1 knockout samples were subjected to SDS-PAGE. ab124465 and Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at a 1/500 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-ASS1 antibody [2B10] (ab124465)

    Predicted band size: 47 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-ASS1 antibody [2B10] (ab124465), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-ASS1 antibody [2B10] (ab124465)

    ab124465 staining ASS1 in wild-type HeLa cells (top panel) and ASS1 knockout HeLa cells (Human ASS1 knockout HeLa cell line ab264989) (bottom panel). The cells were fixed with 100% methanol (5 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab124465 at 1/1000 dilution and Anti-beta Tubulin antibody - Loading Control ab6046 (Rabbit polyclonal to beta Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to mouse IgG (Alexa Fluor® 488) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117) at 2 μg/ml (shown in green) and a goat secondary antibody to rabbit IgG (Alexa Fluor® 594) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
    Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).

  • Immunocytochemistry/ Immunofluorescence - Anti-ASS1 antibody [2B10] (ab124465), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-ASS1 antibody [2B10] (ab124465)

    ab124465, at 1/200 dilution, staining ASS1 in HeLa cells by Immunofluorescence (green). Blue: DRAQ5 fluorescent DNA dye.

  • ELISA - Anti-ASS1 antibody [2B10] (ab124465), expandable thumbnail

    ELISA - Anti-ASS1 antibody [2B10] (ab124465)

    ELISA using ab124465.

  • Western blot - Anti-ASS1 antibody [2B10] (ab124465), expandable thumbnail

    Western blot - Anti-ASS1 antibody [2B10] (ab124465)

    All lanes: Western blot - Anti-ASS1 antibody [2B10] (ab124465) at 1/500 dilution

    Lane 1: A431 cell lysate

    Lane 2: Raji cell lysate

    Lane 3: L1210 cell lysate

    Lane 4: MOLT4 cell lysate

    Lane 5: Jurkat cell lysate

    Lane 6: A549 cell lysate

    Lane 7: NIH 3T3 cell lysate

    Lane 8: PC12 cell lysate

    Lane 9: COS7 cell lysate

    Predicted band size: 47 kDa

  • Western blot - Anti-ASS1 antibody [2B10] (ab124465), expandable thumbnail

    Western blot - Anti-ASS1 antibody [2B10] (ab124465)

    All lanes: Western blot - Anti-ASS1 antibody [2B10] (ab124465) at 1/500 dilution

    All lanes: Human ASS1 recombinant protein (aa: 40-236)

    Predicted band size: 19 kDa, 47 kDa

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