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AB191165

Anti-ASS1 antibody

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(3 Publications)

Rabbit Polyclonal ASS1 antibody. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human ASS1 aa 150-250 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

ASS, ASS1, Argininosuccinate synthase, Citrulline--aspartate ligase

5 Images
Flow Cytometry (Intracellular) - Anti-ASS1 antibody (AB191165)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-ASS1 antibody (AB191165)

Intracellular flow cytometric analysis of WiDr cells labeling ASS1 with ab191165 at 1/10 dilution (lower histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunocytochemistry/ Immunofluorescence - Anti-ASS1 antibody (AB191165)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ASS1 antibody (AB191165)

Immunofluorescent analysis of HeLa cells labeling ASS1 with ab191165 at 1/10 dilution followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASS1 antibody (AB191165)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASS1 antibody (AB191165)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue labeling ASS1 with ab191165 at 1/75 dilution.

Western blot - Anti-ASS1 antibody (AB191165)
  • WB

Supplier Data

Western blot - Anti-ASS1 antibody (AB191165)

All lanes:

Western blot - Anti-ASS1 antibody (ab191165) at 1/1000 dilution

All lanes:

HeLa cell lysate at 35 µg

Predicted band size: 47 kDa

false

Western blot - Anti-ASS1 antibody (AB191165)
  • WB

Supplier Data

Western blot - Anti-ASS1 antibody (AB191165)

All lanes:

Western blot - Anti-ASS1 antibody (ab191165) at 1/1000 dilution

All lanes:

Mouse kidney tissue lysate at 35 µg

Predicted band size: 47 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

ICC/IF, IHC-P, WB, Flow Cyt (Intra)

applications

Immunogen

Synthetic Peptide within Human ASS1 aa 150-250 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P00966

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Precipitation Ammonium Sulphate
Storage buffer
pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ASS1 or argininosuccinate synthase 1 is an enzyme that plays an important role in the urea cycle. It catalyzes the conversion of citrulline and aspartate into argininosuccinate using ATP in the process. The ASS1 protein is commonly expressed in the liver where it actively participates in the detoxification of ammonia. It has a molecular mass of approximately 46 kDa. Some alternate names for this protein include ASS and ASS-1.
Biological function summary

Argininosuccinate synthase 1 contributes significantly to the synthesis of arginine an essential amino acid through its conversion activities. The enzyme operates as a homotrimer a complex consisting of three identical subunits. This enzymatic activity is indispensable in maintaining the balance of nitrogen and ammonia within the body particularly important for liver and kidney functions.

Pathways

ASS1 is a critical component of both the urea cycle and the nitric oxide metabolism pathway. The urea cycle helps in detoxifying ammonia by converting it into urea which is excreted in urine. In nitric oxide metabolism ASS1 links with nitric oxide synthase to facilitate the use of arginine in producing nitric oxide a vital signaling molecule. The pathway engagement partners include proteins like citrin which supplies aspartate for the reaction.

ASS1 has implications in citrullinemia and hepatocellular carcinoma. Citrullinemia results from mutations in the ASS1 gene leading to defective urea cycle function and ammonia accumulation. In cancer particularly hepatocellular carcinoma low expression of ASS1 correlates with poor prognosis as cancer cells often exhibit arginine auxotrophy. Connections through these conditions involve interactions with enzymes such as arginase which also influences arginine availability.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

One of the enzymes of the urea cycle, the metabolic pathway transforming neurotoxic amonia produced by protein catabolism into inocuous urea in the liver of ureotelic animals. Catalyzes the formation of arginosuccinate from aspartate, citrulline and ATP and together with ASL it is responsible for the biosynthesis of arginine in most body tissues.
See full target information ASS1
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cancer & metabolism 12:31 PubMed39462426

2024

GCN2-SLC7A11 axis coordinates autophagy, cell cycle and apoptosis and regulates cell growth in retinoblastoma upon arginine deprivation.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Wang,Wai Kit Chu,Jason Cheuk Sing Yam,Chi Pui Pang,Yun Chung Leung,Alisa Sau Wun Shum,Sun-On Chan

Cell reports. Medicine 4:101073 PubMed37290438

2023

Arginine depletion attenuates renal cystogenesis in tuberous sclerosis complex model.

Applications

Unspecified application

Species

Unspecified reactive species

Athar Amleh,Hadass Pri Chen,Lana Watad,Ifat Abramovich,Bella Agranovich,Eyal Gottlieb,Iddo Z Ben-Dov,Morris Nechama,Oded Volovelsky

Nature communications 13:1783 PubMed35379807

2022

Opposite physiological and pathological mTORC1-mediated roles of the CB1 receptor in regulating renal tubular function.

Applications

Unspecified application

Species

Unspecified reactive species

Liad Hinden,Majdoleen Ahmad,Sharleen Hamad,Alina Nemirovski,Gergő Szanda,Sandra Glasmacher,Aviram Kogot-Levin,Rinat Abramovitch,Bernard Thorens,Jürg Gertsch,Gil Leibowitz,Joseph Tam
View all publications
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Product promise

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