Anti-ASS1 antibody

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ASS1 antibody (AB77590)

Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation with ab77590 for 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control : Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASS1 antibody (AB77590)

IHC image of ab77590 staining in human kidney formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab77590, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-ASS1 antibody (AB77590)

Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation with ab77590 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (1 μg/ml). IgG control : Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-ASS1 antibody (AB77590)

ICC/IF image of ab77590 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween (no animal sera) for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab77590 at 10μg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 donkey anti- goat (ab150133) IgG used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43μM for 1hour at room temperature.

• WB

Supplier Data

Western blot - Anti-ASS1 antibody (AB77590)

Lanes 1 - 2:

Western blot - Anti-ASS1 antibody (ab77590) at 0.01 µg/mL

Lane 3:

Western blot - Anti-ASS1 antibody (ab77590) at 0.03 µg/mL

Lane 1:

Human Kidney at 35 µg

Lane 2:

Mouse Liver at 35 µg

Lane 3:

Rat Kidney at 35 µg

Predicted band size: 47 kDa

false

• WB

Supplier Data

Western blot - Anti-ASS1 antibody (AB77590)

Lane 1:

Western blot - Anti-ASS1 antibody (ab77590) at 0.3 µg/mL

Lane 2:

Western blot - Anti-ASS1 antibody (ab77590) at 1 µg/mL

Lane 1:

A431 cell lysate at 35 µg

Lane 2:

NIH/3T3 cell lysate at 35 µg

Predicted band size: 47 kDa

false