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Rabbit Recombinant Monoclonal ASS1 antibody. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Human, Rat samples. Cited in 10 publications.


Images

Immunoprecipitation - Anti-ASS1 antibody [EPR12398] (AB170952), expandable thumbnail
  • Western blot - Anti-ASS1 antibody [EPR12398] (AB170952), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-ASS1 antibody [EPR12398] (AB170952), expandable thumbnail
  • Western blot - Anti-ASS1 antibody [EPR12398] (AB170952), expandable thumbnail
  • Western blot - Anti-ASS1 antibody [EPR12398] (AB170952), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWBICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Tested
Tested
Tested
Mouse
Tested
Expected
Tested
Expected
Expected
Rat
Expected
Expected
Tested
Expected
Expected
Cow
Predicted
Predicted
Predicted
Predicted
Predicted

Tested
Tested

Species
Mouse
Dilution info
1/4000
Notes

For unpurified use at 1/250 - 1/500.

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/4000
Notes

For unpurified use at 1/250 - 1/500.

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Cow
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/10 - 1/100
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Cow
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/20000
Notes

For unpurified use at 1/1000 - 1/10000.

Species
Rat
Dilution info
1/20000
Notes

For unpurified use at 1/1000 - 1/10000.

Species
Human
Dilution info
1/20000
Notes

For unpurified use at 1/1000 - 1/10000.

Predicted
Predicted

Species
Cow
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50 - 1/100
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Cow
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100
Notes

For unpurified use at 1/500 - 1/1000. Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Cow
Dilution info
-
Notes

-

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

One of the enzymes of the urea cycle, the metabolic pathway transforming neurotoxic amonia produced by protein catabolism into inocuous urea in the liver of ureotelic animals. Catalyzes the formation of arginosuccinate from aspartate, citrulline and ATP and together with ASL it is responsible for the biosynthesis of arginine in most body tissues.

Alternative names

ASS, ASS1, Argininosuccinate synthase, Citrulline--aspartate ligase

Recommended products

Rabbit Recombinant Monoclonal ASS1 antibody. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Human, Rat samples. Cited in 10 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR12398
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

The rat recommendation is based on the WB results. This antibody may not be suitable for IHC with rat samples.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

ASS1 or argininosuccinate synthase 1 is an enzyme that plays an important role in the urea cycle. It catalyzes the conversion of citrulline and aspartate into argininosuccinate using ATP in the process. The ASS1 protein is commonly expressed in the liver where it actively participates in the detoxification of ammonia. It has a molecular mass of approximately 46 kDa. Some alternate names for this protein include ASS and ASS-1.

Biological function summary

Argininosuccinate synthase 1 contributes significantly to the synthesis of arginine an essential amino acid through its conversion activities. The enzyme operates as a homotrimer a complex consisting of three identical subunits. This enzymatic activity is indispensable in maintaining the balance of nitrogen and ammonia within the body particularly important for liver and kidney functions.

Pathways

ASS1 is a critical component of both the urea cycle and the nitric oxide metabolism pathway. The urea cycle helps in detoxifying ammonia by converting it into urea which is excreted in urine. In nitric oxide metabolism ASS1 links with nitric oxide synthase to facilitate the use of arginine in producing nitric oxide a vital signaling molecule. The pathway engagement partners include proteins like citrin which supplies aspartate for the reaction.

Associated diseases and disorders

ASS1 has implications in citrullinemia and hepatocellular carcinoma. Citrullinemia results from mutations in the ASS1 gene leading to defective urea cycle function and ammonia accumulation. In cancer particularly hepatocellular carcinoma low expression of ASS1 correlates with poor prognosis as cancer cells often exhibit arginine auxotrophy. Connections through these conditions involve interactions with enzymes such as arginase which also influences arginine availability.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

19 product images

  • Immunoprecipitation - Anti-ASS1 antibody [EPR12398] (ab170952), expandable thumbnail

    Immunoprecipitation - Anti-ASS1 antibody [EPR12398] (ab170952)

    ab170952 (purified) at 1:60 dilution (5ug) immunoprecipitating ASS1 in HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate.
    Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10ug
    Lane 2 (+): ab170952 & HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab170952 in HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-ASS1 antibody [EPR12398] (ab170952)

    Predicted band size: 47 kDa

  • Western blot - Anti-ASS1 antibody [EPR12398] (ab170952), expandable thumbnail

    Western blot - Anti-ASS1 antibody [EPR12398] (ab170952)

    Lanes 1- 2: Merged signal (red and green). Green - ab170952 observed at 47 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) observed at 37 kDa.

    ab170952 was shown to react with ASS1 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line Human ASS1 knockout HeLa cell line ab264989 (knockout cell lysate Human ASS1 knockout HeLa cell lysate ab257143) was used. Wild-type HeLa and ASS1 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab170952 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-ASS1 antibody [EPR12398] (ab170952) at 1/1000 dilution

    Lane 1: Wild-type HeLa cell lysate at 20 µg

    Lane 2: ASS1 knockout HeLa cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 47 kDa

    Observed band size: 47 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-ASS1 antibody [EPR12398] (ab170952), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-ASS1 antibody [EPR12398] (ab170952)

    ab170952 staining ASS1 in wild-type HeLa cells (top panel) and ASS1 knockout HeLa cells (Human ASS1 knockout HeLa cell line ab264989) (bottom panel). The cells were fixed with 100% methanol (5 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab170952 at 1/100 dilution and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse monoclonal to alpha Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 2 μg/ml (shown in green) and a goat secondary antibody to mouse IgG (Alexa Fluor® 594) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
    Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).

  • Western blot - Anti-ASS1 antibody [EPR12398] (ab170952), expandable thumbnail

    Western blot - Anti-ASS1 antibody [EPR12398] (ab170952)

    Lanes 1 - 4: Merged signal (red and green). Green - ab170952 observed at 47 kDa. Red - loading control, Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484, observed at 37 kDa.

    ab170952 was shown to recognize ASS1 in wild-type HAP1 cells as signal was lost at the expected MW in ASS1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and ASS1 knockout samples were subjected to SDS-PAGE. ab170952 and Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/20000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-ASS1 antibody [EPR12398] (ab170952) at 1/20000 dilution

    Lane 1: Wild-type HAP1 whole cell lysate at 20 µg

    Lane 2: ASS1 knockout HAP1 whole cell lysate at 20 µg

    Lane 3: HepG2 whole cell lysate at 20 µg

    Lane 4: HeLa whole cell lysate at 20 µg

    Predicted band size: 47 kDa

    Observed band size: 47 kDa

  • Western blot - Anti-ASS1 antibody [EPR12398] (ab170952), expandable thumbnail

    Western blot - Anti-ASS1 antibody [EPR12398] (ab170952)

    Blocking and diluting buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-ASS1 antibody [EPR12398] (ab170952) at 0.05 µg/mL

    Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: Human fetal liver lysate at 20 µg

    Lane 3: Mouse liver lysate at 20 µg

    Lane 4: Rat liver lysate at 20 µg

    Lane 5: Mouse kidney lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 47 kDa

    Observed band size: 47 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-ASS1 antibody [EPR12398] (ab170952), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-ASS1 antibody [EPR12398] (ab170952)

    Immunocytochemistry/ Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma cell line) cells labeling ASS1 with Purified ab170952 at 1:100 dilution (10.2μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASS1 antibody [EPR12398] (ab170952), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASS1 antibody [EPR12398] (ab170952)

    ab170952 showing +ve staining in Mouse kidney tissue.

  • Flow Cytometry (Intracellular) - Anti-ASS1 antibody [EPR12398] (ab170952), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-ASS1 antibody [EPR12398] (ab170952)

    Intracellular Flow Cytometry analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling ASS1 with purified ab170952 at 1/100 dilution (10 ug/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Immunocytochemistry/ Immunofluorescence - Anti-ASS1 antibody [EPR12398] (ab170952), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-ASS1 antibody [EPR12398] (ab170952)

    Immunofluorescent analysis of HeLa cells labeling ASS1 using ab170952 at 1/50 dilution (red). DAPI nuclear staining (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASS1 antibody [EPR12398] (ab170952), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASS1 antibody [EPR12398] (ab170952)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse kidney tissue sections labeling ASS1 with Purified ab170952 at 1:4000 dilution (0.25 μg/ml). Heat mediated antigen retrieval was performed using citrate Buffer, PH6. Tissue was counterstained with Hematoxylin. Goat Anti-Rabbit IgG H&L (HRP) ab97051 Goat Anti-Rabbit IgG H&L (HRP)
    secondary antibody was used at 1:500 dilution. PBS instead of the primary antibody was used as the negative control.

  • Immunoprecipitation - Anti-ASS1 antibody [EPR12398] (ab170952), expandable thumbnail

    Immunoprecipitation - Anti-ASS1 antibody [EPR12398] (ab170952)

    Secondary antibody used is HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    All lanes: Immunoprecipitation - Anti-ASS1 antibody [EPR12398] (ab170952) at 1/10 dilution

    Lane 1: Human fetal liver tissue lysate at 10 µg

    Lane 2: PBS

    Predicted band size: 47 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASS1 antibody [EPR12398] (ab170952), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASS1 antibody [EPR12398] (ab170952)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human kidney tissue sections labeling ASS1 with Purified ab170952 at 1:4000 dilution (0.25 μg/ml). Heat mediated antigen retrieval was performed using citrate Buffer, PH6. Tissue was counterstained with Hematoxylin. Goat Anti-Rabbit IgG H&L (HRP) ab97051 Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1:500 dilution. PBS instead of the primary antibody was used as the negative control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASS1 antibody [EPR12398] (ab170952), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASS1 antibody [EPR12398] (ab170952)

    ab170952 showing +ve staining in Human normal ureter tissue.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASS1 antibody [EPR12398] (ab170952), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASS1 antibody [EPR12398] (ab170952)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ASS1 with ab170952 at 1/250 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASS1 antibody [EPR12398] (ab170952), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASS1 antibody [EPR12398] (ab170952)

    ab170952 showing -ve staining in Human cervical carcinoma tissue.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASS1 antibody [EPR12398] (ab170952), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASS1 antibody [EPR12398] (ab170952)

    ab170952 showing -ve staining in Human normal uterus tissue.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASS1 antibody [EPR12398] (ab170952), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ASS1 antibody [EPR12398] (ab170952)

    ab170952 showing -ve staining in Human normal pancreas tissue.

  • Flow Cytometry (Intracellular) - Anti-ASS1 antibody [EPR12398] (ab170952), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-ASS1 antibody [EPR12398] (ab170952)

    Intracellular flow cytometric analysis of permeabilized Hela cells labeling ASS1 using ab170952 at 1/500 dilution (red) or a rabbit IgG negative (green).

  • Western blot - Anti-ASS1 antibody [EPR12398] (ab170952), expandable thumbnail

    Western blot - Anti-ASS1 antibody [EPR12398] (ab170952)

    All lanes: Western blot - Anti-ASS1 antibody [EPR12398] (ab170952) at 1/1000 dilution

    Lane 1: HeLa cell lysate at 10 µg

    Lane 2: Fetal liver tissue lysate at 10 µg

    Lane 3: Fetal kidney tissue lysate at 10 µg

    Secondary

    All lanes: Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 47 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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