Anti-ASS1 antibody [EPR12399(B)] - BSA and Azide free

• WB

Lab

Western blot - Anti-ASS1 antibody [EPR12399(B)] - BSA and Azide free (AB249577)

This data was developed using the same antibody clone in a different buffer formulation (ab170900).

Lanes 1- 2 : Merged signal (red and green). Green - ab170900 observed at 47 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

ab170900 was shown to react with ASS1 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab264989 (knockout cell lysate ab257143) was used. Wild-type HeLa and ASS1 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab170900 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-ASS1 antibody [EPR12399(B)] - C-terminal (<a href='/en-us/products/primary-antibodies/ass1-antibody-epr12399b-c-terminal-ab170900'>ab170900</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

ASS1 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human ASS1 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-ass1-knockout-hela-cell-line-ab264989'>ab264989</a>)

Predicted band size: 47 kDa

Observed band size: 47 kDa

false

• WB

Lab

Western blot - Anti-ASS1 antibody [EPR12399(B)] - BSA and Azide free (AB249577)

This data was developed using the same antibody clone in a different buffer formulation (ab170900).

Lanes 1 - 4 : Merged signal (red and green). Green - ab170900 observed at 47 kDa. Red - loading control, ab9484, observed at 37 kDa.

ab170900 was shown to recognize ASS1 when ASS1 knockout samples were used, along with additional cross-reactive bands. Wild-type and ASS1 knockout samples were subjected to SDS-PAGE. ab170900 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-ASS1 antibody [EPR12399(B)] - BSA and Azide free (ab249577) at 1/1000 dilution

Lane 1:

Wild-type HAP1 whole cell lysate at 20 µg/mL

Lane 2:

ASS1 knockout HAP1 whole cell lysate at 20 µg/mL

Lane 3:

HeLa whole cell lysate at 20 µg/mL

Lane 4:

Jurkat whole cell lysate at 20 µg/mL

Predicted band size: 47 kDa

false