Rabbit Recombinant Monoclonal ASS1 antibody. Carrier free. Suitable for IP, WB and reacts with Mouse, Human samples.
Images
![Western blot - Anti-ASS1 antibody [EPR12399(B)] - BSA and Azide free (AB249577), expandable thumbnail](https://content.abcam.com/products/images/ass1-antibody-epr12399b-bsa-and-azide-free-ab249577--western-blot-img145429.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ASS1 antibody [EPR12399(B)] - BSA and Azide free (AB249577)")
![Western blot - Anti-ASS1 antibody [EPR12399(B)] - BSA and Azide free (AB249577), expandable thumbnail](https://content.abcam.com/products/images/ass1-antibody-epr12399b-bsa-and-azide-free-ab249577--western-blot-img139424.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ASS1 antibody [EPR12399(B)] - BSA and Azide free (AB249577)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | IHC-P | |
|---|---|---|---|---|
| Human | Expected | Not recommended | Tested | Not recommended |
| Mouse | Expected | Not recommended | Predicted | Not recommended |
| Rat | Predicted | Not recommended | Predicted | Not recommended |
IP
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
Target data
Function
One of the enzymes of the urea cycle, the metabolic pathway transforming neurotoxic amonia produced by protein catabolism into inocuous urea in the liver of ureotelic animals. Catalyzes the formation of arginosuccinate from aspartate, citrulline and ATP and together with ASL it is responsible for the biosynthesis of arginine in most body tissues.
Alternative names
ASS, ASS1, Argininosuccinate synthase, Citrulline--aspartate ligase
Recommended products
Rabbit Recombinant Monoclonal ASS1 antibody. Carrier free. Suitable for IP, WB and reacts with Mouse, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR12399(B)
- Purification technique
- Affinity purification
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab249577 is the carrier-free version of Anti-ASS1 antibody [EPR12399(B)] - C-terminal ab170900.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ASS1 or argininosuccinate synthase 1 is an enzyme that plays an important role in the urea cycle. It catalyzes the conversion of citrulline and aspartate into argininosuccinate using ATP in the process. The ASS1 protein is commonly expressed in the liver where it actively participates in the detoxification of ammonia. It has a molecular mass of approximately 46 kDa. Some alternate names for this protein include ASS and ASS-1.
- Biological function summary
Argininosuccinate synthase 1 contributes significantly to the synthesis of arginine an essential amino acid through its conversion activities. The enzyme operates as a homotrimer a complex consisting of three identical subunits. This enzymatic activity is indispensable in maintaining the balance of nitrogen and ammonia within the body particularly important for liver and kidney functions.
- Pathways
ASS1 is a critical component of both the urea cycle and the nitric oxide metabolism pathway. The urea cycle helps in detoxifying ammonia by converting it into urea which is excreted in urine. In nitric oxide metabolism ASS1 links with nitric oxide synthase to facilitate the use of arginine in producing nitric oxide a vital signaling molecule. The pathway engagement partners include proteins like citrin which supplies aspartate for the reaction.
- Associated diseases and disorders
ASS1 has implications in citrullinemia and hepatocellular carcinoma. Citrullinemia results from mutations in the ASS1 gene leading to defective urea cycle function and ammonia accumulation. In cancer particularly hepatocellular carcinoma low expression of ASS1 correlates with poor prognosis as cancer cells often exhibit arginine auxotrophy. Connections through these conditions involve interactions with enzymes such as arginase which also influences arginine availability.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-ASS1 antibody [EPR12399(B)] - BSA and Azide free (ab249577), expandable thumbnail](https://content.abcam.com/products/images/ass1-antibody-epr12399b-bsa-and-azide-free-ab249577--western-blot-img145429.jpg "Western blot - Anti-ASS1 antibody [EPR12399(B)] - BSA and Azide free (ab249577)")
Western blot - Anti-ASS1 antibody [EPR12399(B)] - BSA and Azide free (ab249577)
This data was developed using the same antibody clone in a different buffer formulation (Anti-ASS1 antibody [EPR12399(B)] - C-terminal ab170900).
Lanes 1- 2: Merged signal (red and green). Green - Anti-ASS1 antibody [EPR12399(B)] - C-terminal ab170900 observed at 47 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) observed at 37 kDa.
Anti-ASS1 antibody [EPR12399(B)] - C-terminal ab170900 was shown to react with ASS1 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line Human ASS1 knockout HeLa cell line ab264989 (knockout cell lysate Human ASS1 knockout HeLa cell lysate ab257143) was used. Wild-type HeLa and ASS1 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. Anti-ASS1 antibody [EPR12399(B)] - C-terminal ab170900 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-ASS1 antibody [EPR12399(B)] - C-terminal (Anti-ASS1 antibody [EPR12399(B)] - C-terminal ab170900) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: ASS1 knockout HeLa cell lysate at 20 µg
Lane 2: Western blot - Human ASS1 knockout HeLa cell line (Human ASS1 knockout HeLa cell line ab264989)
Performed under reducing conditions.
Predicted band size: 47 kDa
Observed band size: 47 kDa
![Western blot - Anti-ASS1 antibody [EPR12399(B)] - BSA and Azide free (ab249577), expandable thumbnail](https://content.abcam.com/products/images/ass1-antibody-epr12399b-bsa-and-azide-free-ab249577--western-blot-img139424.jpg "Western blot - Anti-ASS1 antibody [EPR12399(B)] - BSA and Azide free (ab249577)")
Western blot - Anti-ASS1 antibody [EPR12399(B)] - BSA and Azide free (ab249577)
This data was developed using the same antibody clone in a different buffer formulation (Anti-ASS1 antibody [EPR12399(B)] - C-terminal ab170900).
Lanes 1 - 4: Merged signal (red and green). Green - Anti-ASS1 antibody [EPR12399(B)] - C-terminal ab170900 observed at 47 kDa. Red - loading control, Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484, observed at 37 kDa.
Anti-ASS1 antibody [EPR12399(B)] - C-terminal ab170900 was shown to recognize ASS1 when ASS1 knockout samples were used, along with additional cross-reactive bands. Wild-type and ASS1 knockout samples were subjected to SDS-PAGE. Anti-ASS1 antibody [EPR12399(B)] - C-terminal ab170900 and Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-ASS1 antibody [EPR12399(B)] - BSA and Azide free (ab249577) at 1/1000 dilution
Lane 1: Wild-type HAP1 whole cell lysate at 20 µg/mL
Lane 2: ASS1 knockout HAP1 whole cell lysate at 20 µg/mL
Lane 3: HeLa whole cell lysate at 20 µg/mL
Lane 4: Jurkat whole cell lysate at 20 µg/mL
Predicted band size: 47 kDa
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