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Rabbit Polyclonal Astrin/Deepest antibody. Suitable for ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Sperm-associated antigen 5 aa 900 to C-terminus.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-Astrin/Deepest antibody (AB184142), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human Sperm-associated antigen 5 aa 900 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q96R06

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IF
Human
Tested

Tested
Tested

Species

Human

Dilution info

1/100.00000 - 1/1000.00000

Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Essential component of the mitotic spindle required for normal chromosome segregation and progression into anaphase (PubMed:11724960, PubMed:12356910, PubMed:27462074). Required for chromosome alignment, normal timing of sister chromatid segregation, and maintenance of spindle pole architecture (PubMed:17664331, PubMed:27462074). In complex with SKAP, promotes stable microtubule-kinetochore attachments. May contribute to the regulation of separase activity. May regulate AURKA localization to mitotic spindle, but not to centrosomes and CCNB1 localization to both mitotic spindle and centrosomes (PubMed:18361916, PubMed:21402792). Involved in centriole duplication. Required for CDK5RAP2, CEP152, WDR62 and CEP63 centrosomal localization and promotes the centrosomal localization of CDK2 (PubMed:26297806). In non-mitotic cells, upon stress induction, inhibits mammalian target of rapamycin complex 1 (mTORC1) association and recruits the mTORC1 component RPTOR to stress granules (SGs), thereby preventing mTORC1 hyperactivation-induced apoptosis (PubMed:23953116). May enhance GSK3B-mediated phosphorylation of other substrates, such as MAPT/TAU (PubMed:18055457).

Alternative names

Recommended products

Rabbit Polyclonal Astrin/Deepest antibody. Suitable for ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Sperm-associated antigen 5 aa 900 to C-terminus.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human Sperm-associated antigen 5 aa 900 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q96R06
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Astrin also known as SPAG5 or Deepest is a protein important for cell division. It has a molecular mass of approximately 45 kDa. Researchers find Astrin primarily in the cytosol and preferably expressed in proliferating cells. Astrin plays a significant role in spindle stabilization during mitosis binding to microtubules to ensure proper chromosome segregation. This protein has become a focus of study due to its critical functions during cell cycle progression.

Biological function summary

Astrin facilitates kinetochore-microtubule attachment participating in the mitotic spindle assembly complex. This complex ensures chromosome alignment and segregation which is critical in cellular replication. Since precise regulation of this process is necessary for genetic stability Astrin's function is indispensable. Its presence influences the structural integrity of dividing cells linking it to the stability of genetic information passed to daughter cells after division.

Pathways

Astrin operates within the spindle assembly checkpoint and cell cycle pathways. The spindle assembly checkpoint pathway ensures that cells do not progress to anaphase until all chromosomes are properly attached to the spindle apparatus. In synergy with proteins like Ndc80 and KNL1 Astrin maintains attachment integrity. Its interactions with these proteins ensure accurate chromosome segregation protecting against chromosomal instability which can lead to aneuploidy.

Associated diseases and disorders

Astrin's role connects it to conditions like cancer and developmental disorders. Overexpression of Astrin has associations with tumor progression since its function affects cell proliferation. In the context of cancer proteins like Aurora B also influence Astrin's activity. The misregulation of this pathway can lead to faulty chromosomal alignment and segregation contributing to oncogenesis. Such insights make Astrin a potential biomarker and therapeutic target in cancer research.

Product promise

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1 product image

  • Immunocytochemistry/ Immunofluorescence - Anti-Astrin/Deepest antibody (ab184142), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Astrin/Deepest antibody (ab184142)

    Immunofluorescent analysis of HeLa cells (fixed in 100% methanol for 5 mins) labeling Astrin/Deepest with ab184142 at 1/500 dilution (green). Blue: Hoechst 33342 staining.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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