AB241581

Anti-Astrin/Deepest antibody

Name: Anti-Astrin/Deepest antibody (ab241581) | Abcam
Brand: Abcam Limited
SKU: AB241581
Price: 520 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal Astrin/Deepest antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human Sperm-associated antigen 5 aa 850-950.

View Alternative Names

Sperm-associated antigen 5, Astrin, Deepest, Mitotic spindle-associated protein p126, MAP126, SPAG5

2 Images

Supplier Data

Western blot - Anti-Astrin/Deepest antibody (AB241581)

All lanes:

Western blot - Anti-Astrin/Deepest antibody (ab241581) at 0.04 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Predicted band size: 134 kDa

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Exposure time: 30s

Supplier Data

Immunoprecipitation - Anti-Astrin/Deepest antibody (AB241581)

Astrin/Deepest was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241581 at 3 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab241581 at 0.1 μg/ml.

Lane 1 : ab241581 IP in HeLa whole cell lysate.

Lane 2 : Control IgG IP in HeLa whole cell lysate.

Detection : Chemiluminescence with exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-Astrin/Deepest antibody (ab241581)

Predicted band size: 134 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human Sperm-associated antigen 5 aa 850-950. The exact immunogen used to generate this antibody is proprietary information.

Q96R06

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab241581 was affinity purified using an epitope specific to Astrin/Deepest immobilized on solid support.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Astrin also known as SPAG5 or Deepest is a protein important for cell division. It has a molecular mass of approximately 45 kDa. Researchers find Astrin primarily in the cytosol and preferably expressed in proliferating cells. Astrin plays a significant role in spindle stabilization during mitosis binding to microtubules to ensure proper chromosome segregation. This protein has become a focus of study due to its critical functions during cell cycle progression.

Biological function summary

Astrin facilitates kinetochore-microtubule attachment participating in the mitotic spindle assembly complex. This complex ensures chromosome alignment and segregation which is critical in cellular replication. Since precise regulation of this process is necessary for genetic stability Astrin's function is indispensable. Its presence influences the structural integrity of dividing cells linking it to the stability of genetic information passed to daughter cells after division.

Pathways

Astrin operates within the spindle assembly checkpoint and cell cycle pathways. The spindle assembly checkpoint pathway ensures that cells do not progress to anaphase until all chromosomes are properly attached to the spindle apparatus. In synergy with proteins like Ndc80 and KNL1 Astrin maintains attachment integrity. Its interactions with these proteins ensure accurate chromosome segregation protecting against chromosomal instability which can lead to aneuploidy.

Astrin's role connects it to conditions like cancer and developmental disorders. Overexpression of Astrin has associations with tumor progression since its function affects cell proliferation. In the context of cancer proteins like Aurora B also influence Astrin's activity. The misregulation of this pathway can lead to faulty chromosomal alignment and segregation contributing to oncogenesis. Such insights make Astrin a potential biomarker and therapeutic target in cancer research.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Essential component of the mitotic spindle required for normal chromosome segregation and progression into anaphase (PubMed : 11724960, PubMed : 12356910, PubMed : 27462074). Required for chromosome alignment, normal timing of sister chromatid segregation, and maintenance of spindle pole architecture (PubMed : 17664331, PubMed : 27462074). In complex with SKAP, promotes stable microtubule-kinetochore attachments. May contribute to the regulation of separase activity. May regulate AURKA localization to mitotic spindle, but not to centrosomes and CCNB1 localization to both mitotic spindle and centrosomes (PubMed : 18361916, PubMed : 21402792). Involved in centriole duplication. Required for CDK5RAP2, CEP152, WDR62 and CEP63 centrosomal localization and promotes the centrosomal localization of CDK2 (PubMed : 26297806). In non-mitotic cells, upon stress induction, inhibits mammalian target of rapamycin complex 1 (mTORC1) association and recruits the mTORC1 component RPTOR to stress granules (SGs), thereby preventing mTORC1 hyperactivation-induced apoptosis (PubMed : 23953116). May enhance GSK3B-mediated phosphorylation of other substrates, such as MAPT/TAU (PubMed : 18055457).

See full target information Sperm-associated antigen 5

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Disease markers 2022:5085183 PubMed36118675

2022

Curcumin Improves Keratinocyte Proliferation, Inflammation, and Oxidative Stress through Mediating the SPAG5/FOXM1 Axis in an Model of Actinic Dermatitis by Ultraviolet.

Applications

Unspecified application

Species

Unspecified reactive species

Quan Chen,Yi Tang,Huiyan Deng,Bihua Liang,Huaping Li,Zhenjie Li,Huilan Zhu,Lezi Chen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Astrin/Deepest antibody

Western blot - Anti-Astrin/Deepest antibody (AB241581)

Immunoprecipitation - Anti-Astrin/Deepest antibody (AB241581)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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