AB323503

Anti-Astrin/Deepest antibody [EPR28134-51]

Recombinant
20ul selling size
RabMAb
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Rabbit Recombinant Monoclonal Astrin/Deepest antibody. Suitable for WB and reacts with Human samples.

View Alternative Names

Sperm-associated antigen 5, Astrin, Deepest, Mitotic spindle-associated protein p126, MAP126, SPAG5

4 Images

Supplier Data

Western blot - Anti-Astrin/Deepest antibody [EPR28134-51] (AB323503)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 30842647, PMID : 33288550, PMID : 23953116).

To minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Lanes 1-2 : 26 seconds, lane 3 : 10seconds

All lanes:

Western blot - Anti-Astrin/Deepest antibody [EPR28134-51] (ab323503) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) transfected with scrambled siRNA control whole cell lysate at 50 µg

Lane 2:

293T transfected with siRNA specifically targeting Astrin/Deepest whole cell lysate at 50 µg

Lane 3:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 135 kDa,150 kDa,36 kDa

false

Supplier Data

Western blot - Anti-Astrin/Deepest antibody [EPR28134-51] (AB323503)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The molecular weight of Astrin/Deepest is upshifted in response to STLC treatment (PMID : 33288550).

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 30842647, PMID : 33288550, PMID : 23953116).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Astrin/Deepest antibody [EPR28134-51] (ab323503) at 1/1000 dilution

Lane 1:

Untreated HeLa whole cell lysate at 20 µg

Lane 2:

HeLa treated with 10 µM STLC for 14 hours whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/100000 dilution

Observed band size: 135 kDa,150 kDa,36 kDa

false

Exposure time: 180s

Supplier Data

Western blot - Anti-Astrin/Deepest antibody [EPR28134-51] (AB323503)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The molecular weight of Astrin/Deepest is upshifted in response to nocodazole treatment (PMID : 33288550, PMID : 23953116).

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 30842647, PMID : 33288550, PMID : 23953116).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Astrin/Deepest antibody [EPR28134-51] (ab323503) at 1/1000 dilution

Lane 1:

Untreated HeLa whole cell lysate at 20 µg

Lane 2:

HeLa treated with 100 ng/ml nocodazole for 20 hours whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/20000 dilution

Observed band size: 135 kDa,150 kDa,36 kDa

false

Exposure time: 92s

Supplier Data

Western blot - Anti-Astrin/Deepest antibody [EPR28134-51] (AB323503)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : lung.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 30842647, PMID : 33288550, PMID : 23953116).

The identity of bands lower than 50 kDa are unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Astrin/Deepest antibody [EPR28134-51] (ab323503) at 1/1000 dilution

Lane 1:

Human testis tissue lysate at 80 µg

Lane 2:

Human lung tissue lysate at 80 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 135 kDa,150 kDa,36 kDa

false

Exposure time: 59s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR28134-51

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Essential component of the mitotic spindle required for normal chromosome segregation and progression into anaphase (PubMed : 11724960, PubMed : 12356910, PubMed : 27462074). Required for chromosome alignment, normal timing of sister chromatid segregation, and maintenance of spindle pole architecture (PubMed : 17664331, PubMed : 27462074). In complex with SKAP, promotes stable microtubule-kinetochore attachments. May contribute to the regulation of separase activity. May regulate AURKA localization to mitotic spindle, but not to centrosomes and CCNB1 localization to both mitotic spindle and centrosomes (PubMed : 18361916, PubMed : 21402792). Involved in centriole duplication. Required for CDK5RAP2, CEP152, WDR62 and CEP63 centrosomal localization and promotes the centrosomal localization of CDK2 (PubMed : 26297806). In non-mitotic cells, upon stress induction, inhibits mammalian target of rapamycin complex 1 (mTORC1) association and recruits the mTORC1 component RPTOR to stress granules (SGs), thereby preventing mTORC1 hyperactivation-induced apoptosis (PubMed : 23953116). May enhance GSK3B-mediated phosphorylation of other substrates, such as MAPT/TAU (PubMed : 18055457).

See full target information Sperm-associated antigen 5

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com