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Rabbit Recombinant Monoclonal Astrin/Deepest antibody. Suitable for WB and reacts with Human samples.

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Images

Western blot - Anti-Astrin/Deepest antibody [EPR28134-51] (AB323503), expandable thumbnail
  • Western blot - Anti-Astrin/Deepest antibody [EPR28134-51] (AB323503), expandable thumbnail
  • Western blot - Anti-Astrin/Deepest antibody [EPR28134-51] (AB323503), expandable thumbnail
  • Western blot - Anti-Astrin/Deepest antibody [EPR28134-51] (AB323503), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIPICC/IFIHC-P
Human
Tested
Not recommended
Not recommended
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse
Dilution info
-
Notes

-

Target data

Function

Essential component of the mitotic spindle required for normal chromosome segregation and progression into anaphase (PubMed:11724960, PubMed:12356910, PubMed:27462074). Required for chromosome alignment, normal timing of sister chromatid segregation, and maintenance of spindle pole architecture (PubMed:17664331, PubMed:27462074). In complex with SKAP, promotes stable microtubule-kinetochore attachments. May contribute to the regulation of separase activity. May regulate AURKA localization to mitotic spindle, but not to centrosomes and CCNB1 localization to both mitotic spindle and centrosomes (PubMed:18361916, PubMed:21402792). Involved in centriole duplication. Required for CDK5RAP2, CEP152, WDR62 and CEP63 centrosomal localization and promotes the centrosomal localization of CDK2 (PubMed:26297806). In non-mitotic cells, upon stress induction, inhibits mammalian target of rapamycin complex 1 (mTORC1) association and recruits the mTORC1 component RPTOR to stress granules (SGs), thereby preventing mTORC1 hyperactivation-induced apoptosis (PubMed:23953116). May enhance GSK3B-mediated phosphorylation of other substrates, such as MAPT/TAU (PubMed:18055457).

Alternative names

Sperm-associated antigen 5, Astrin, Deepest, Mitotic spindle-associated protein p126, MAP126, SPAG5

Rabbit Recombinant Monoclonal Astrin/Deepest antibody. Suitable for WB and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR28134-51
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-Astrin/Deepest antibody [EPR28134-51] (ab323503), expandable thumbnail

    Western blot - Anti-Astrin/Deepest antibody [EPR28134-51] (ab323503)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression: lung.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 30842647, PMID: 33288550, PMID: 23953116).

    The identity of bands lower than 50 kDa are unknown.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-Astrin/Deepest antibody [EPR28134-51] (ab323503) at 1/1000 dilution

    Lane 1: Human testis tissue lysate at 80 µg with NFDM/TBST

    Lane 2: Human lung tissue lysate at 80 µg with NFDM/TBST

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 135,150 kDa, 36 kDa

    Exposure time: 59s

  • Western blot - Anti-Astrin/Deepest antibody [EPR28134-51] (ab323503), expandable thumbnail

    Western blot - Anti-Astrin/Deepest antibody [EPR28134-51] (ab323503)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The molecular weight of Astrin/Deepest is upshifted in response to nocodazole treatment (PMID: 33288550, PMID: 23953116).

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 30842647, PMID: 33288550, PMID: 23953116).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-Astrin/Deepest antibody [EPR28134-51] (ab323503) at 1/1000 dilution

    Lane 1: Untreated HeLa whole cell lysate at 20 µg with NFDM/TBST

    Lane 2: HeLa treated with 100 ng/ml nocodazole for 20 hours whole cell lysate at 20 µg with NFDM/TBST

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/20000 dilution

    Observed band size: 135,150 kDa, 36 kDa

    Exposure time: 92s

  • Western blot - Anti-Astrin/Deepest antibody [EPR28134-51] (ab323503), expandable thumbnail

    Western blot - Anti-Astrin/Deepest antibody [EPR28134-51] (ab323503)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 30842647, PMID: 33288550, PMID: 23953116).

    To minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    Exposure time: Lanes 1-2: 26 seconds, lane 3: 10seconds

    All lanes: Western blot - Anti-Astrin/Deepest antibody [EPR28134-51] (ab323503) at 1/1000 dilution

    Lane 1: 293T (human embryonic kidney epithelial cell) transfected with scrambled siRNA control whole cell lysate at 50 µg with NFDM/TBST

    Lane 2: 293T transfected with siRNA specifically targeting Astrin/Deepest whole cell lysate at 50 µg with NFDM/TBST

    Lane 3: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 50 µg with NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 135,150 kDa, 36 kDa

  • Western blot - Anti-Astrin/Deepest antibody [EPR28134-51] (ab323503), expandable thumbnail

    Western blot - Anti-Astrin/Deepest antibody [EPR28134-51] (ab323503)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The molecular weight of Astrin/Deepest is upshifted in response to STLC treatment (PMID: 33288550).

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 30842647, PMID: 33288550, PMID: 23953116).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-Astrin/Deepest antibody [EPR28134-51] (ab323503) at 1/1000 dilution

    Lane 1: Untreated HeLa whole cell lysate at 20 µg with NFDM/TBST

    Lane 2: HeLa treated with 10 µM STLC for 14 hours whole cell lysate at 20 µg with NFDM/TBST

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/100000 dilution

    Observed band size: 135,150 kDa, 36 kDa

    Exposure time: 180s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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