AB50817

Anti-ASXL1 antibody [2049C2a]

Name: Anti-ASXL1 antibody [2049C2a] (ab50817) | Abcam
Brand: Abcam Limited
SKU: AB50817

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(1 Publication)

Mouse Monoclonal ASXL1 antibody. Suitable for WB and reacts with Recombinant fragment - Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ASXL1.

View Alternative Names

KIAA0978, ASXL1, Polycomb group protein ASXL1, Additional sex combs-like protein 1

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

2049C2a

Isotype

IgG1

Carrier free

Applications

applications

Immunogen

Recombinant Fragment Protein within Human ASXL1. The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Recombinant fragment - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.

Storage buffer

pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

Up to 12 months

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ASXL1 also known as Additional Sex Combs-Like 1 functions primarily as a core component in chromatin modification processes. This protein has a molecular mass of approximately 165 kDa. ASXL1 is typically expressed in a wide range of tissues including hematopoietic cells indicating its role in diverse biological processes. The protein serves as a regulator of gene expression by influencing how tightly DNA wraps around histones allowing or restricting access to transcriptional machinery.

Biological function summary

ASXL1 plays a critical role in epigenetic regulation and is often part of polycomb repressive complexes. These complexes contribute to transcriptional repression particularly during cellular differentiation and development. ASXL1 interacts with other proteins to modify histones thereby affecting the expression of genes involved in growth and differentiation processes. Its function is closely connected to developmental pathways given its impact on regulation at the chromatin level.

Pathways

ASXL1 interacts with complex epigenetic regulatory networks. It takes part in the PRC2 (Polycomb Repressive Complex 2) pathway an essential pathway for transcriptional silencing via histone methylation. ASXL1 also shows interactions with proteins like EZH2 and SUZ12 within this pathway contributing to the methylation of histone H3 at lysine 27 (H3K27me3). Such interactions underline its role in chromatin remodeling and maintaining gene silencing during development and homeostasis.

ASXL1 mutations are frequently linked to myeloid malignancies such as myelodysplastic syndromes (MDS) and chronic myelomonocytic leukemia (CMML). These mutations often result in loss of function disrupting gene expression regulation and leading to abnormal cellular proliferation. ASXL1’s role connects with the protein RUNX1 in these conditions where impaired regulation can progress to malignancies. Researchers continue to investigate the exact mechanisms through which these interactions contribute to the pathogenesis of these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Probable Polycomb group (PcG) protein involved in transcriptional regulation mediated by ligand-bound nuclear hormone receptors, such as retinoic acid receptors (RARs) and peroxisome proliferator-activated receptor gamma (PPARG) (PubMed : 16606617). Acts as a coactivator of RARA and RXRA through association with NCOA1 (PubMed : 16606617). Acts as a corepressor for PPARG and suppresses its adipocyte differentiation-inducing activity (By similarity). Non-catalytic component of the PR-DUB complex, a complex that specifically mediates deubiquitination of histone H2A monoubiquitinated at 'Lys-119' (H2AK119ub1) (PubMed : 20436459, PubMed : 30664650, PubMed : 36180891). Acts as a sensor of N(6)-methyladenine methylation on DNA (6mA) : recognizes and binds 6mA DNA, leading to its ubiquitination and degradation by TRIP12, thereby inactivating the PR-DUB complex and regulating Polycomb silencing (PubMed : 30982744). The PR-DUB complex is an epigenetic regulator of gene expression and acts as a transcriptional coactivator, affecting genes involved in development, cell communication, signaling, cell proliferation and cell viability (PubMed : 30664650, PubMed : 36180891). ASXL1, ASXL2 and ASXL3 function redundantly in the PR-DUB complex (By similarity) (PubMed : 30664650). The ASXL proteins are essential for chromatin recruitment and transcriptional activation of associated genes (By similarity). ASXL1 and ASXL2 are important for BAP1 protein stability (PubMed : 30664650). Together with BAP1, negatively regulates epithelial-mesenchymal transition (EMT) of trophoblast stem cells during placental development by regulating genes involved in epithelial cell integrity, cell adhesion and cytoskeletal organization (PubMed : 34170818).

See full target information ASXL1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 6:7307 PubMed26095772

2015

Cancer-associated ASXL1 mutations may act as gain-of-function mutations of the ASXL1-BAP1 complex.

Applications

Species

Mouse

Anand Balasubramani,Antti Larjo,Jed A Bassein,Xing Chang,Ryan B Hastie,Susan M Togher,Harri Lähdesmäki,Anjana Rao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-ASXL1 antibody [2049C2a]

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise