AB244431

Anti-ATAD2 antibody

Name: Anti-ATAD2 antibody (ab244431) | Abcam
Brand: Abcam Limited
SKU: AB244431
Price: 630 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal ATAD2 antibody. Suitable for IHC-P, ICC, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ATAD2 aa 1050 to C-terminus.

View Alternative Names

L16, PRO2000, ATAD2, ATPase family AAA domain-containing protein 2, AAA nuclear coregulator cancer-associated protein, ANCCA

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATAD2 antibody (AB244431)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATAD2 antibody (AB244431)

Immunohistochemical (Formalin/PFA-fixed paraffin-embedded sections) analysis in human lymph node tissue labeling ATAD2 with ab244431 at 1/500 dilution. Antigen retrieval was heat mediated with citrate buffer pH 6.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATAD2 antibody (AB244431)

Immunohistochemical (Formalin/PFA-fixed paraffin-embedded sections) analysis in human bone marrow tissue labeling ATAD2 with ab244431 at 1/500 dilution. Antigen retrieval was heat mediated with citrate buffer pH 6.0 before commencing with IHC staining protocol.

Unknown

Immunocytochemistry - Anti-ATAD2 antibody (AB244431)

Immunocytochemistry of PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for ATAD2 (green) using ab244431 at 4 μg/ml.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATAD2 antibody (AB244431)

Immunohistochemical (Formalin/PFA-fixed paraffin-embedded sections) analysis in human testis tissue labeling ATAD2 with ab244431 at 1/500 dilution. Antigen retrieval was heat mediated with citrate buffer pH 6.0 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-ATAD2 antibody (AB244431)

All lanes:

Western blot - Anti-ATAD2 antibody (ab244431) at 0.4 µg/mL

Lane 1:

U-2 OS (human bone osteosarcoma epithelial cell line) plus target-specific siRNA#1, cell lysate

Lane 2:

U-2 OS plus target-specific siRNA#2, cell lysate

Lane 3:

U-2 OS plus control siRNA, cell lysate

Predicted band size: 159 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, ICC, WB

applications

Immunogen

Recombinant Fragment Protein within Human ATAD2 aa 1050 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q6PL18

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "0.25-2 µg/mL", "ICC-species-notes": "Fixation/Permeabilization: PFA/Triton X-100.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATAD2 also known as ATPase family AAA domain-containing protein 2 is a chromatin-associated AAA ATPase. This protein weighs approximately 157 kDa and functions mainly in the cell nucleus. ATAD2 is widely expressed in various tissues with high levels found in the testis indicating its possible role in testicular functions. It shows ATPase activity involving the hydrolysis of ATP which provides energy for chromatin remodeling processes.

Biological function summary

ATAD2 plays a critical role in transcription regulation by remodeling chromatin structure. As part of a complex it interacts with histone acetylation marks on chromatin therefore influencing gene expression. The protein is often involved in the regulation of genes relevant to cell cycle progression. Its interaction with transcriptional regulators highlights its importance in controlling proliferation and other cellular processes.

Pathways

The role of ATAD2 becomes essential in pathways like the cell cycle and the DNA damage response. Through these pathways it interacts with key proteins such as MYC a known regulator of cell growth and it influences the expression of genes involved in cell cycle control. It is important in maintaining genomic stability by ensuring proper chromatin dynamics during DNA replication and repair.

Aberrant expression of ATAD2 associates with cancer progression particularly in breast cancer where it is highly expressed in MCF7 cell lines. It is also implicated in hepatocellular carcinoma where it interacts with cancer-related proteins such as p53 which is a well-known tumor suppressor. These interactions suggest that ATAD2 serves as a potential target for cancer therapies focused on disrupting its interactions or functions in tumor biology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May be a transcriptional coactivator of the nuclear receptor ESR1 required to induce the expression of a subset of estradiol target genes, such as CCND1, MYC and E2F1. May play a role in the recruitment or occupancy of CREBBP at some ESR1 target gene promoters. May be required for histone hyperacetylation. Involved in the estrogen-induced cell proliferation and cell cycle progression of breast cancer cells.

See full target information ATAD2

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

International journal of biological sciences 20:200-217 PubMed38164169

2024

Treatment for type 2 diabetes and diabetic nephropathy by targeting Smad3 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Huijun He,Honglian Wang,Xiaocui Chen,Yu Zhong,Xiao Ru Huang,Ronald Cw Ma,Cheng Wang,Hui-Yao Lan

American journal of physiology. Cell physiology 319:C93-C104 PubMed31747529

2019

Downregulation of AAA-domain-containing protein 2 restrains cancer stem cell properties in esophageal squamous cell carcinoma via blockade of the Hedgehog signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Nuo Li,Yang Yu,Baoming Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ATAD2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATAD2 antibody (AB244431)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATAD2 antibody (AB244431)

Immunocytochemistry - Anti-ATAD2 antibody (AB244431)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATAD2 antibody (AB244431)

Western blot - Anti-ATAD2 antibody (AB244431)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise