Anti-ATAD2 antibody [EPR12730] - BSA and Azide free

• WB

Lab

Western blot - Anti-ATAD2 antibody [EPR12730] - BSA and Azide free (AB249904)

This data was developed using the same antibody clone in a different buffer formulation (ab176319).

Lanes 1- 2 : Merged signal (red and green). Green - ab176319 observed at 200 kDa. Red - Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) observed at 50 kDa.

ab176319 was shown to react with ATAD2 in wild-type HeLa cells in western blot. The band observed in CRISPR/Cas9 edited cell line ab264957 (CRISPR/Cas9 edited cell lysate ab257359) lane below 200kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type HeLa and ATAD2 CRISPR/Cas9 edited HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab176319 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-ATAD2 antibody [EPR12730] (<a href='/en-us/products/primary-antibodies/atad2-antibody-epr12730-ab176319'>ab176319</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

ATAD2 CRISPR/Cas9 edited HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human ATAD2 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-atad2-knockout-hela-cell-line-ab264957'>ab264957</a>)

Predicted band size: 159 kDa

Observed band size: 200 kDa

false