Knockout Tested Rabbit Recombinant Monoclonal ATAD2 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples.
pH: 7.2 - 7.4
Constituents: PBS
IP | WB | IHC-P | ICC/IF | Flow Cyt (Intra) | |
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Human | Not recommended | Tested | Not recommended | Expected | Expected |
Species | Dilution info | Notes |
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Species Human | Dilution info - | Notes - |
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Species Human | Dilution info - | Notes - |
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Species Human | Dilution info - | Notes - |
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Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
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Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
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May be a transcriptional coactivator of the nuclear receptor ESR1 required to induce the expression of a subset of estradiol target genes, such as CCND1, MYC and E2F1. May play a role in the recruitment or occupancy of CREBBP at some ESR1 target gene promoters. May be required for histone hyperacetylation. Involved in the estrogen-induced cell proliferation and cell cycle progression of breast cancer cells.
L16, PRO2000, ATAD2, ATPase family AAA domain-containing protein 2, AAA nuclear coregulator cancer-associated protein, ANCCA
Knockout Tested Rabbit Recombinant Monoclonal ATAD2 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples.
pH: 7.2 - 7.4
Constituents: PBS
ab249904 is the carrier-free version of Anti-ATAD2 antibody [EPR12730] ab176319.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
ATAD2 also known as ATPase family AAA domain-containing protein 2 is a chromatin-associated AAA ATPase. This protein weighs approximately 157 kDa and functions mainly in the cell nucleus. ATAD2 is widely expressed in various tissues with high levels found in the testis indicating its possible role in testicular functions. It shows ATPase activity involving the hydrolysis of ATP which provides energy for chromatin remodeling processes.
ATAD2 plays a critical role in transcription regulation by remodeling chromatin structure. As part of a complex it interacts with histone acetylation marks on chromatin therefore influencing gene expression. The protein is often involved in the regulation of genes relevant to cell cycle progression. Its interaction with transcriptional regulators highlights its importance in controlling proliferation and other cellular processes.
The role of ATAD2 becomes essential in pathways like the cell cycle and the DNA damage response. Through these pathways it interacts with key proteins such as MYC a known regulator of cell growth and it influences the expression of genes involved in cell cycle control. It is important in maintaining genomic stability by ensuring proper chromatin dynamics during DNA replication and repair.
Aberrant expression of ATAD2 associates with cancer progression particularly in breast cancer where it is highly expressed in MCF7 cell lines. It is also implicated in hepatocellular carcinoma where it interacts with cancer-related proteins such as p53 which is a well-known tumor suppressor. These interactions suggest that ATAD2 serves as a potential target for cancer therapies focused on disrupting its interactions or functions in tumor biology.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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This data was developed using the same antibody clone in a different buffer formulation (Anti-ATAD2 antibody [EPR12730] ab176319).
Lanes 1- 2: Merged signal (red and green). Green - Anti-ATAD2 antibody [EPR12730] ab176319 observed at 200 kDa. Red - Anti-alpha Tubulin antibody [DM1A] - Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) observed at 50 kDa.
Anti-ATAD2 antibody [EPR12730] ab176319 was shown to react with ATAD2 in wild-type HeLa cells in western blot. The band observed in CRISPR/Cas9 edited cell line Human ATAD2 knockout HeLa cell line ab264957 (CRISPR/Cas9 edited cell lysate Human ATAD2 knockout HeLa cell lysate ab257359) lane below 200kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type HeLa and ATAD2 CRISPR/Cas9 edited HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. Anti-ATAD2 antibody [EPR12730] ab176319 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) were incubated overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-ATAD2 antibody [EPR12730] (Anti-ATAD2 antibody [EPR12730] ab176319) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: ATAD2 CRISPR/Cas9 edited HeLa cell lysate at 20 µg
Lane 2: Western blot - Human ATAD2 knockout HeLa cell line (Human ATAD2 knockout HeLa cell line ab264957)
Performed under reducing conditions.
Predicted band size: 159 kDa
Observed band size: 200 kDa
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